Results 51 to 60 of about 19,699 (283)

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases

BMC Biotechnology, 2008
Background Luciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simultaneously using green- and red ...
Ohmiya Yoshihiro, Ikeda Masaaki, Noguchi Takako, Nakajima Yoshihiro   +3 more
doaj   +1 more source

Introducing a new reporter gene, membrane-anchored Cypridina luciferase, for multiplex bioluminescence imaging

Molecular Therapy: Oncolytics, 2021
Bioluminescence reporter gene imaging is a robust, high-throughput imaging modality that is useful for tracking cells and monitoring biological processes, both in cell culture and in small animals.
Maxim A. Moroz, Juan Zurita, Anna Moroz, Ekaterina Nikolov, Yury Likar, Konstantin Dobrenkov, Jason Lee, Larissa Shenker, Ronald Blasberg, Inna Serganova, Vladimir Ponomarev   +10 more
doaj   +1 more source

Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging [PDF]

, 2018
The sensitivity of bioluminescence imaging in animals is primarily dependent on the amount of photons emitted by the luciferase enzyme at wavelengths greater than 620 nm where tissue penetration is high.
Encell, L.P. (Lance P.), Hall, M.P. (Mary P.), Kirkland, T.A. (Thomas A.), Löwik, C.W.G.M. (Clemens), Mezzanotte, L. (Laura), Que, I. (Ivo), Ridwan, R.Y. (Yanto), Root, M. (Moniek) van 't, Shi, C. (Ce), Wood, K.V. (Keith V.), Wood, M.G. (Monika G.), Woodroofe, C.C. (Carolyn C.)   +11 more
core   +1 more source

Emerging role of ARHGAP29 in melanoma cell phenotype switching

Molecular Oncology, EarlyView.
This study gives first insights into the role of ARHGAP29 in malignant melanoma. ARHGAP29 was revealed to be connected to tumor cell plasticity, promoting a mesenchymal‐like, invasive phenotype and driving tumor progression. Further, it modulates cell spreading by influencing RhoA/ROCK signaling and affects SMAD2 activity. Rho GTPase‐activating protein
Beatrice Charlotte Tröster, Melanie Kappelmann‐Fenzl, Anja Katrin Bosserhoff, Nicole Rachinger   +3 more
wiley   +1 more source

Bioluminescence emissions of firefly _Luciola praeusta_ Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae) [PDF]

, 2008
_In vivo_ emission and time-resolved spectra of firefly _Luciola praeusta_ Kiesenwetter 1874 (Coleoptera : Lampyridae : Luciolinae) have been recorded.
Anurup Gohain Barua, Gauranga Dhar Baruah, Nayanmoni Saikia, Simanta Hazarika   +3 more
core   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Convergent synthesis and optical properties of near-infrared emitting bioluminescent infra-luciferins [PDF]

, 2016
Infra-luciferin, an alkene linked analogue of luciferin, gives bioluminescence emission >700 nm and has the potential to be used for multiparametric in vivo imaging.
Anderson, James C., Grounds, Helen, Jathoul, Amit, Murray, James Augustus Henry, Pacman, Steven J., Tisi, Laurence   +5 more
core   +12 more sources

High-sensitivity real-time imaging of dual protein-protein interactions in living subjects using multicolor luciferases. [PDF]

PLoS ONE, 2009
Networks of protein-protein interactions play key roles in numerous important biological processes in living subjects. An effective methodology to assess protein-protein interactions in living cells of interest is protein-fragment complement assay (PCA).
Naoki Hida, Muhammad Awais, Masaki Takeuchi, Naoto Ueno, Mayuri Tashiro, Chiyo Takagi, Tanuja Singh, Makoto Hayashi, Yoshihiro Ohmiya, Takeaki Ozawa   +9 more
doaj   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source