Results 71 to 80 of about 171,488 (262)
Effects of diabetes on cardiac lysosomes and protein degradation
, 1983 Rates of proteolysis by hearts obtained from alloxan diabetic rats and perfused as working preparations with buffer simulating control sera were accelerated 30% above identically perfused control hearts. The total homogenate activities of cathepsin D and
N. Lautensack +4 more
core +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
HISTOCHEMISTRY OF LYSOSOMES IN RAT HEART MUSCLE
, 1967 Rat heart muscle was studied histochemically by the naphthol and lead methods for hydrolases (acid phosphatase, esterase, β-glucuronidase and aryl sulfatase). Activity of the enzymes studied is demonstrable in granules which by their staining properties
MARGARET MORRIS +2 more
core +1 more source
Correlative imaging data to locate lysosomes in cells
, 2021 Correlative imaging data to locate lysosomes in the cell, including Cryo-SXT data and GSDIM ...
Haobo Bai (11727227)
core +1 more source
The Emerging Role of the Lysosome in Parkinson’s Disease
Cells, 2020 Lysosomal function has a central role in maintaining neuronal homeostasis, and, accordingly, lysosomal dysfunction has been linked to neurodegeneration and particularly to Parkinson’s disease (PD).
Alba Navarro-Romero +2 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta +7 more
wiley +1 more source
Lysosomes of rat liver and kidney. [PDF]
, 2018 Fractionation techniques, based on zonal centrifugation in density gradients, were developed for examining the heterogeneity of lysosomal populations and also for the purification of lysosomes from normal rat liver.
Dobrota, Miloslav.
core
Type I interferons modulate autophagy to shape gemcitabine response in pancreatic cancer cells
FEBS Open Bio, EarlyView.Type I interferons differentially modulate autophagy and the response of pancreatic cancer cells to gemcitabine. IFNα2b stimulates autophagic flux and protects cells from gemcitabine‐induced cell death, contributing to chemoresistance. In contrast, IFNβ1a inhibits autophagosome formation and enhances gemcitabine‐induced cell death, resulting in ...
Lucy E. Bonilla +10 more
wiley +1 more source
Activity-dependent trafficking of lysosomes in dendrites and dendritic spines [PDF]
, 2017 In neurons, lysosomes, which degrade membrane and cytoplasmic components, are thought to primarily reside in somatic and axonal compartments, but there is little understanding of their distribution and function in dendrites.
Natalia Slepak +15 more
core +1 more source