Results 71 to 80 of about 168,690 (311)

Continuous transport of a small fraction of plasma membrane cholesterol to endoplasmic reticulum regulates total cellular cholesterol

eLife, 2017
Cells employ regulated transport mechanisms to ensure that their plasma membranes (PMs) are optimally supplied with cholesterol derived from uptake of low-density lipoproteins (LDL) and synthesis.
Rodney Elwood Infante, Arun Radhakrishnan   +1 more
doaj   +1 more source

Effects of methylcyclodextrin on lysosomes

, 2001
The cholesterol complexing agent methyl-cyclodextrin (MCD) provides an efficient mean for the removal of cholesterol from biological membranes. In order to study the effects of this agent on the lysosomal membrane in situ, we treated HepG2 cells with MCD
Jadot, Michel, Andrianaivo, Fanjambolatiana, Wattiaux, Robert, Dubois, Franz   +3 more
core   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

The Emerging Role of the Lysosome in Parkinson’s Disease

Cells, 2020
Lysosomal function has a central role in maintaining neuronal homeostasis, and, accordingly, lysosomal dysfunction has been linked to neurodegeneration and particularly to Parkinson’s disease (PD).
Alba Navarro-Romero, Marta Montpeyó, Marta Martinez-Vicente   +2 more
doaj   +1 more source

Liver lysosomes in protein-starved rats

, 1969
1. The feeding of a protein-free diet to adult rats trained to consume their whole day's food in 2 h changed the characteristics of hepatic lysosomes immediately upon the onset of protein deficiency combined with calorie deficiency. 2.
Saroja, S., Padwaldesai, S. R., Sawant, P. L., Kumta, U. S., Sreenivasan, A., Ninjoor, V.   +5 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Interaction of rat liver lysosomes with basic polypeptides [PDF]

, 1995
In order to gain knowledge on the interaction of lysosomes with proteins, we have assessed the equilibrium densities of the lysosomal membrane and matrix markers after in vitro incubation of rat liver lysosomes with various polypeptides.
Rukavishnikova, Elena V., Toshihiro Sassa, Tatjana A. Korolenko, Korolenko, Tatjana A., Horiuchi, Saburou, Oka, Tatsuzo, Sassa, Toshihiro, Saburou Horiuchi, Elena V. Rukavishnikova, Natoria, Yasuo, Tatsuzo Oka, Yasuo Natoria   +11 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

RUNDC1 negatively mediates the fusion of autophagosomes with lysosomes via regulating SNARE complex assembly

Macroautophagy/autophagy is an essential pro-survival mechanism activated in response to nutrient deficiency. The proper fusion between autophagosomes and lysosomes is a critical step for autophagic degradation.
Zhou, C., Lyu, Hao, Xiao, Shuai, Torraca, V., Guo, Dong, Tang, J., Zhang, Rui   +6 more
core   +1 more source