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Validation of methyl malondialdehyde as internal standard for malondialdehyde detection by capillary electrophoresis

Analytical Biochemistry, 2002
The aim of this study was to validate, by capillary electrophoresis, the use of synthesized methyl malondialdehyde as the internal standard for the direct quantification of free and total (free+bound) malondialdehyde in biological samples. All analyses were performed in 20 cm x 50 microm uncoated capillaries at 20 degrees C, using 25 mmol/L borax (pH 9.
Rita Paroni   +2 more
exaly   +3 more sources

Kinetic Modeling of Malondialdehyde Reactivity in Oil to Simulate Actual Malondialdehyde Formation upon Lipid Oxidation

Proceedings of the Virtual 2021 AOCS Annual Meeting & Expo, 2021
The reactivity of malondialdehyde in saturated glycerol triheptanoate oil was studied over a wide temperature range (298.15-453.15 K). With respect to the non-ideal character of a lipid medium, a kinetic model was proposed that described the experimental malondialdehyde data by a reversible hydrolytic cleavage and an irreversible aldol self ...
Angelique, Vandemoortele   +3 more
openaire   +3 more sources

Malondialdehyde Assays in Higher Plants

Methods in molecular biology
Malondialdehyde is a three-carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation widely used as a marker of the extent of lipid peroxidation in plants. There are several methodological approaches to quantify malondialdehyde contents in higher plants, ranging from the simplest, cheapest, and quickest spectrophotometric ...
Melanie, Morales, Sergi, Munné-Bosch
openaire   +3 more sources

Behavior of Malondialdehyde in Oil-in-Water Emulsions

Journal of Agricultural and Food Chemistry, 2015
The impact of temperature, emulsifier, and protein type on the reactivity of malondialdehyde in oil-in-water emulsions was elucidated. Malondialdehyde recoveries in aqueous buffer, protein solutions, saturated oil, and fully hydrogenated coconut oil-in-water emulsions stabilized by whey proteins or Tween 20 at 4 or 40 °C were compared.
Angelique Vandemoortele   +1 more
exaly   +3 more sources

Cleavage and Oligomerization of Malondialdehyde

Tetrahedron, 1993
Abstract Malondialdehyde (MDA) slowly self-condensates in aqueous solution at pH 4.5–6 and room temperature yielding (E)-(3′-oxo-1′-propenyl)malondialdehyde and 2,4-dihydroxymethylene-3-(2′-oxoethyl)glutaraldehyde. The latter compound exists in solution in equilibrium with 4-(1,3-dioxopropan-2-yl)-5-formyl-2-hydroxy-3,4-dihydro-2H-pyran, the trans ...
Inés Maya
exaly   +2 more sources

Malondialdehyde epitopes as mediators of sterile inflammation

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2017
Enhanced lipid peroxidation occurs during oxidative stress and results in the generation of lipid peroxidation end products such as malondialdehyde (MDA), which can attach to autologous biomolecules, thereby generating neo-self epitopes capable of inducing potentially undesired biological responses. Therefore, the immune system has developed mechanisms
Clara J, Busch, Christoph J, Binder
openaire   +3 more sources

Lipid peroxidation as measured by chromatographic determination of malondialdehyde. Human plasma reference values in health and disease.

Archives of Biochemistry and Biophysics, 2021
Free radicals and oxidants are involved in physiological signaling pathways, although an imbalance between pro-oxidant and anti-oxidant systems in favor of the former leads to major biomolecular damage.
C. Mas-Bargues   +4 more
semanticscholar   +1 more source

Lipid peroxidation—DNA damage by malondialdehyde

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1999

exaly   +2 more sources

Methyl malondialdehyde as an internal standard for the determination of malondialdehyde

Journal of Chromatography B: Biomedical Sciences and Applications, 2001
Methyl malondialdehyde (Me-MDA) is suggested as an internal standard for the determination of the lipid peroxidation product, malondialdehyde (MDA). A procedure for synthesising the Me-MDA sodium salt is described in detail. The purity and identity of the synthesised Me-MDA have been confirmed using nuclear magnetic resonance and UV spectroscopy, and ...
K, Claeson, G, Thorsén, B, Karlberg
openaire   +2 more sources

Effects of oxidative modification by malondialdehyde on the in vitro digestion properties of rice bran protein

, 2021
As a secondary oxidation product derived during rice bran oxidative rancidity, malondialdehyde (MDA) was selected to study the impact of oxidative rancidity on the digestion properties of rice bran protein.
Fang Li, Xiaojuan Wu, Wei Wu
semanticscholar   +1 more source

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