Retrospective studies involving the screening of frozen stored collections of samples are commonplace when a new threat emerges, but it has been demonstrated that the freeze-thaw process can affect bacterial viability.
Pedro Miguela-Villoldo +12 more
doaj +1 more source
Antimicrobial resistance and mcr-1 gene in Escherichia coli isolated from poultry samples submitted to a bacteriology laboratory in South Africa [PDF]
Background and Aim: Antimicrobial resistance (AMR) and recently mobilized colistin resistance (mcr-1) associated colistin resistance among Escherichia coli isolates have been attributed to the overuse of antimicrobials in livestock production. E.
Ibrahim Z. Hassan +4 more
doaj +1 more source
Molecular detection of colistin resistance genes (mcr-1 to mcr-5) in human vaginal swabs
Objective Colistin resistance has emerged worldwide and has been threatening the efficacy of one of the last-resort antimicrobials used for treatment of multidrug resistant Gram-negative bacteria. While five colistin resistance genes (mcr-1, mcr-2, mcr-3,
Jilei Zhang +8 more
doaj +1 more source
Solid-Phase PCR-Amplified DNAzyme Activity for Real-Time FO-SPR Detection of the MCR-2 Gene
The polymerase chain reaction (PCR) has been the gold standard molecular analysis technique for decades and has seen quite some evolution in terms of reaction components, methodology, and readout mechanisms. Nucleic acid enzymes (NAzymes) have been used to further exploit the applications of PCR, but so far the work was limited to the colorimetric G ...
Peeters, Bernd +5 more
openaire +4 more sources
Mobile colistin-resistant genes (mcr) have become an increasing public health concern. Since the first report of mcr-1 in Thailand in 2016, perspective surveillance was conducted to explore the genomic characteristics of clinical carbapenem-resistant ...
Wantana Paveenkittiporn +3 more
doaj +1 more source
Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program. [PDF]
A set of 908 clinically derived colistin-resistant Enterobacteriaeae isolates collected worldwide in 2014-2016 were screened for the presence of the plasmid-borne mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 genes.
Mark G Wise +4 more
doaj +1 more source
The prevalence and transmission of mobile colistin resistance (mcr) genes have led to a severe threat to humans and animals. Escherichia fergusonii is an emerging pathogen which is closely related to a variety of diseases.
Ruishan Liu +8 more
doaj +1 more source
Evaluation of a New Commercial Microarray Platform for the Simultaneous Detection of β-Lactamase and mcr-1 and mcr-2 Genes in Enterobacteriaceae [PDF]
The worldwide prevalence of infections due to Gram-negative bacteria coresistant to extended-spectrum cephalosporins and carbapenems is worrisome. For these organisms, polymyxins are considered the last-resort antibacterials.
Odette J. Bernasconi +6 more
openaire +2 more sources
mcr-1 and mcr-2 variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015. [PDF]
To determine the occurrence of mcr-1 and mcr-2 genes in Gram-negative bacteria isolated from healthy pigs in Great Britain.Gram-negative bacteria (n = 657) isolated from pigs between 2014 and 2015 were examined by WGS.Variants of mcr-1 and mcr-2 were identified in Moraxella spp.
AbuOun, M +11 more
openaire +5 more sources
Colistin resistant Enterobacteriaceae have emerged in humans, food producing animals and food. The aim of this study was to assess the occurrence of MCR-1 and MCR-2 producers among Enterobacteriaceae isolated from companion animals admitted to the University of Zurich veterinary clinic.
Simmen, Sabrina +3 more
openaire +3 more sources

