Results 21 to 30 of about 12,869 (202)
Biomolecular condensates mediate bending and scission of endosome membranes [PDF]
NatureAbstractMultivesicular bodies are key endosomal compartments implicated in cellular quality control through their degradation of membrane-bound cargo proteins1–3. The ATP-consuming ESCRT protein machinery mediates the capture and engulfment of membrane-bound cargo proteins through invagination and scission of multivesicular-body membranes to form ...
Yanning Wang +14 more
core +8 more sources
Influenza virus M2 protein mediates ESCRT-independent membrane scission. [PDF]
Cell, 2010 Many viruses utilize host ESCRT proteins for budding; however, influenza virus budding is thought to be ESCRT-independent. In this study we have found a role for the influenza virus M2 proton-selective ion channel protein in mediating virus budding. We observed that a highly conserved amphipathic helix located within the M2 cytoplasmic tail mediates a ...
Rossman JS, Jing X, Leser GP, Lamb RA.
europepmc +6 more sources
FAM134B oligomerization drives endoplasmic reticulum membrane scission for ER-phagy. [PDF]
EMBO J, 2020 Degradation of endoplasmic reticulum (ER) by selective autophagy (ER-phagy) is crucial for ER homeostasis. However, it remains unclear how ER scission is regulated for subsequent autophagosomal sequestration and lysosomal degradation. Here, we show that oligomerization of ER-phagy receptor FAM134B (also referred to as reticulophagy regulator 1 or ...
Jiang X +15 more
europepmc +4 more sources
The final twist in endocytic membrane scission [PDF]
Nature Cell Biology, 2021 Many endocytic uptake events depend on the 'pinchase' activity of dynamin. By measuring the orientation of single gold nanorods, a new study reveals that invaginated clathrin-coated endocytic pits undergo a strong rotational twist prior to or concomitant with their detachment.
Johannes, Ludger, Valades-Cruz, Cesar
openaire +3 more sources
A helical assembly of human ESCRT-I scaffolds reverse-topology membrane scission. [PDF]
Nat Struct Mol Biol, 2020 The ESCRT complexes drive membrane scission in HIV-1 release, autophagosome closure, multivesicular body biogenesis, cytokinesis, and other cell processes. ESCRT-I is the most upstream complex and bridges the system to HIV-1 Gag in virus release.
Flower TG +11 more
europepmc +3 more sources
, 2022 The filament goes through a series of composition and geometry changes, from the Flat Spiral to the Wide Helix, and then to a constricting Tight Helix. A: In the absence of cargo, the membrane breaks at the bottom tip of the neck and retracts after the ...
Elene Lominadze (13970470) +7 more
core +1 more source
Dynamin recruitment and membrane scission at the neck of a clathrin-coated pit. [PDF]
Mol Biol Cell, 2014 Dynamin, the GTPase required for clathrin-mediated endocytosis, is recruited to clathrin-coated pits in two sequential phases. The first is associated with coated pit maturation; the second, with fission of the membrane neck of a coated pit. Using gene-edited cells that express dynamin2-EGFP instead of dynamin2 and live-cell TIRF imaging with single ...
Cocucci E, Gaudin R, Kirchhausen T.
europepmc +5 more sources
Dynamin: Membrane Scission Meets Physics [PDF]
Current Biology, 2012 Dynamin hydrolyzes GTP to constrict and sever membranes. Experimental advances bring dynamin into the realm of physics and reveal key roles for membrane tension and bending at the edge of the constriction.
Hurley, James H., Hinshaw, Jenny E.
openaire +2 more sources
Subdomains of endophilin can drive membrane remodeling and facilitate controlled membrane scission. [PDF]
Biophys JBeiter JR +3 more
europepmc +2 more sources
A new role for myosin II in vesicle fission. [PDF]
PLoS ONE, 2014 An endocytic vesicle is formed from a flat plasma membrane patch by a sequential process of invagination, bud formation and fission. The scission step requires the formation of a tubular membrane neck (the fission pore) that connects the endocytic ...
Juan A Flores +5 more
doaj +1 more source