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Metagenomics Using Next-Generation Sequencing
2014Traditionally, microbial genome sequencing has been restricted to the small number of species that can be grown in pure culture. The progressive development of culture-independent methods over the last 15 years now allows researchers to sequence microbial communities directly from environmental samples.
Bragg,Lauren, Tyson, Gene W.
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Exploring Metagenomes Using Next-Generation Sequencing
2017Metagenomics or community genomics refers to the study of genomic DNA of any culture-independent analysis of microbial communities. Non-culturable microbial groups represent the huge majority of global microorganisms. Microbial populations present in every biological niche even humans body carry 10 times more bacterial cells and 100 times more ...
Jalpa R. Thakkar +2 more
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Rapid detection of pulmonary nocardiosis by metagenomic next generation sequencing
Diagnostic Microbiology and Infectious Disease, 2023We retrospectively analyzed 3 cases of pulmonary nocardiosis. Patients were identified by metagenomic next generation sequencing (mNGS) in the First Affiliated Hospital of Soochow University, from January 2019 to April 2022. All patients had underlying diseases, and were in immunocompromised state, with membranous nephropathy, acute lymphoblastic ...
Lirong Gao +4 more
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Metagenomic Next-Generation Sequencing of Viruses Infecting Grapevines
2015Next-generation sequencing (NGS) technologies, for the first time, provide a truly "complete" representation of the viral (and other) pathogens present in a host organism. This is achieved in an unbiased way, and without any prior biological or molecular knowledge of these pathogen(s).
Johan T, Burger, Hans J, Maree
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Application of metagenomic next-generation sequencing in suspected intraocular infections
European Journal of Ophthalmology, 2022Purpose To evaluate the efficacy of metagenomic next-generation sequencing (mNGS) and to explore its value in the diagnosis of intraocular infection. Methods A retrospective case-series study of patients with suspected intraocular infection was performed. Intraocular fluid samples were collected from all the patients and sent for mNGS.
Zhuyun Qian +8 more
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Metagenomic next-generation sequencing for the diagnosis of neurobrucellosis
Future MicrobiologyObjective: This study investigates the application of metagenomic next-generation sequencing (mNGS) in the diagnosis of neurobrucellosis (NB). Methods: We retrospectively analyzed patients diagnosed with NB who underwent cerebrospinal fluid (CSF) mNGS testing in Xijing Hospital from 2015 to 2021. Results: Among the 20 individuals included in the study,
Wen Li +7 more
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Metagenomic next-generation sequencing clinches diagnosis of leishmaniasis
The Lancet, 2021Chong, Wang +3 more
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Metagenome assembly through clustering of next-generation sequencing data using protein sequences
Journal of Microbiological Methods, 2015The study of environmental microbial communities, called metagenomics, has gained a lot of attention because of the recent advances in next-generation sequencing (NGS) technologies. Microbes play a critical role in changing their environments, and the mode of their effect can be solved by investigating metagenomes.
Mikang, Sim, Jaebum, Kim
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Application of Metagenomic Next-Generation Sequencing
Background: Metagenomic high-throughput sequencing (mNGS) represents a powerful tool for detecting nucleic acids from various pathogens, such as bacteria, fungi, viruses and parasites, in clinical samples. Despite its extensively employed in the pathogen diagnosis for various infectious diseases, its application in diagnosing stroke-related infection ...openaire +1 more source
Diagnosis of Mycobacterium marinum Infection by Metagenomic next-generation sequencing
Clinical LaboratoryIn December 2023, our hospital confirmed a case of finger infection with Mycobacterium marinum. The patient sought medical attention at our hospital due to a hard scratch on her left middle finger, which was red, swollen, and ulcerated for one month.A lesion of approximately 1.5 cm x 2 cm in the patient's left middle finger, surrounded by redness and ...
Yan, Zhong, Yun, Xing
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