Results 31 to 40 of about 23,484 (216)

Vitamin Bs as Potent Anticancer Agents through MMP-2/9 Regulation

Frontiers in Bioscience-Landmark
In recent years, the role of coenzymes, particularly those from the vitamin B group in modulating the activity of metalloenzymes has garnered significant attention in cancer treatment strategies.
Ha Vy Thi Vo, Namdoo Kim, Hyuck Jin Lee
doaj   +1 more source

Structural insights into the effect of active-site mutation on the catalytic mechanism of carbonic anhydrase

IUCrJ, 2020
Enzymes are catalysts of biological processes. Significant insight into their catalytic mechanisms has been obtained by relating site-directed mutagenesis studies to kinetic activity assays. However, revealing the detailed relationship between structural
Jin Kyun Kim, Cheol Lee, Seon Woo Lim, Jacob T. Andring, Aniruddha Adhikari, Robert McKenna, Chae Un Kim   +6 more
doaj   +1 more source

Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature

IUCrJ, 2022
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies.
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Šrajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough   +25 more
doaj   +1 more source

Computational strategies for the design of new enzymatic functions [PDF]

, 2015
In this contribution, recent developments in the design of biocatalysts are reviewed with particular emphasis in the de novo strategy. Studies based on three different reactions, Kemp elimination, Diels–Alder and Retro-Aldolase, are used to illustrate ...
Bertrán, Juan, Moliner, Vicent, Tuñón, Iñaki, Świderek, Katarzyna   +3 more
core   +2 more sources

Two distinct ferredoxins are essential for nitrogen fixation by the iron nitrogenase in Rhodobacter capsulatus

mBio
Nitrogenases are the only enzymes able to fix gaseous nitrogen into bioavailable ammonia and hence are essential for sustaining life. Catalysis by nitrogenases requires both a large amount of ATP and electrons donated by strongly reducing ferredoxins or ...
Holly Addison, Timo Glatter, Georg K. A. Hochberg, Johannes G. Rebelein   +3 more
doaj   +1 more source

Comparison of the amine/amino acid activation profiles of the β- and γ-carbonic anhydrases from the pathogenic bacterium Burkholderia pseudomallei

Journal of Enzyme Inhibition and Medicinal Chemistry, 2018
The β-class carbonic anhydrase (CA, EC 4.2.1.1) from the pathogenic bacterium Burkholderia pseudomallei, BpsCAβ, that is responsible for the tropical disease melioidosis was investigated for its activation with natural and non-natural amino acids and ...
Daniela Vullo, Sonia Del Prete, Sameh M. Osman, Fatmah A. S. Alasmary, Zeid AlOthman, William A. Donald, Clemente Capasso, Claudiu T. Supuran   +7 more
doaj   +1 more source

Mineral composition of Aegle marmelos (L.) corrêa ash: Exploration of its ability to mimic the function of different metalloenzymes

Chemistry of Inorganic Materials
The motivation to find functional models of different metalloenzymes comes from their potential to develop better catalysts inspired by nature. Generally, metalloenzymes can activate aerial dioxygen due to the metal ions in it.
Kousik Ghosh, K. Vamsee Krishna, T. Vinodkumar, T. Dinesh, R. Balakumar, S. Chitra   +5 more
doaj   +1 more source

Cloning, expression and purification of the α-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis

Journal of Enzyme Inhibition and Medicinal Chemistry, 2017
We cloned, expressed, purified, and determined the kinetic constants of the recombinant α-carbonic anhydrase (rec-MgaCA) identified in the mantle tissue of the bivalve Mediterranean mussel, Mytilus galloprovincialis.
Rosa Perfetto, Sonia Del Prete, Daniela Vullo, Vincenzo Carginale, Giovanni Sansone, Carmela M. A. Barone, Mosè Rossi, Fatmah A. S. Alasmary, Sameh M. Osman, Zeid AlOthman, Claudiu T. Supuran, Clemente Capasso   +11 more
doaj   +1 more source

Sulfonamide Inhibition Profile of the β-Carbonic Anhydrase from Malassezia restricta, An Opportunistic Pathogen Triggering Scalp Conditions

Metabolites, 2020
The critical CO2 hydration reaction to bicarbonate and protons is catalyzed by carbonic anhydrases (CAs, EC 4.2.1.1). Their physiological role is to assist the transport of the CO2 and HCO3− at the cellular level, which will not be ensured by the ...
Sonia Del Prete, Andrea Angeli, Cynthia Ghobril, Julien Hitce, Cécile Clavaud, Xavier Marat, Claudiu T. Supuran, Clemente Capasso   +7 more
doaj   +1 more source

To what extent do structural changes in catalytic metal sites affect enzyme function? [PDF]

, 2017
About half of known enzymatic reactions involve metals. Enzymes belonging to the same superfamily often evolve to catalyze different reactions on the same structural scaffold.
Andreini, Claudia, Furnham, Nicholas, Rosato, Antonio, Thornton, Janet M, Valasatava, Yana   +4 more
core   +2 more sources