METTL3-mediated long non-coding RNA MIR99AHG methylation targets miR-4660 to promote bone marrow mesenchymal stem cell osteogenic differentiation [PDF]
, 2022 Whether long non-coding RNA Mir-99a-Let-7c Cluster Host Gene (LncRNA MIR99AHG) is involved in osteoporosis (OP) remains vague, so we hereby center on its implication. Old C57BL/6J mice were injected with the silencing lentivirus of MIR99AHG and subjected
Jianning Zhao (795284) +7 more
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Previously described Mettl3 KO mESC lines express shorter isoforms of Mettl3. [PDF]
, 2022 (A) Mettl3 KO mESCs from two groups have different m6A levels. To reconfirm the m6A levels using quantitative methods, we performed mass spectrometry to estimate the m6A in mRNA.
Hui Xian Poh (13118985) +3 more
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Cry2-mCh-METTL3 interaction with EGFP-METTL3/14. [PDF]
, 2022 (A) Co-immunoprecipitation assay with HEK293T coexpressing Cry2-mCh-METTL3 and EGFP-tagged METTL3 or METTL14. IB, immunoblot; IP, immunoprecipitation; METTL3, methyltransferase-like 3; METTL14, methyltransferase-like 14; WCL, whole cell lysate; WTAP ...
Vivian Hoang (12072856) +5 more
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METTL3-mediated m6A is required for murine oocyte maturation and maternal-to-zygotic transition [PDF]
, 2020 N6-methyladenosine (m6A) is the most prevalent epigenetic modification of messenger RNA (mRNA) in higher eukaryotes; this modification is mainly catalyzed by a methyltransferase complex including methyltransferase-like 3 (METTL3) as a key factor ...
Qiqi Cao (6249212) +8 more
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Polysome profiling reveals that exon2 Mettl3 KO mESCs translate Mettl3 mRNAs. [PDF]
, 2022 To investigate if the altered Mettl3 mRNAs expressed in the exon2 Mettl3 KO mESCs are translated, we performed polysome profiling. The polysomes were separated into 4 fractions based on their ribosome load: Fraction 1 (sub-monosomal), Fraction 2 (low ...
Hui Xian Poh (13118985) +3 more
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Shortened isoforms of METTL3 catalyze m6A formation in Mettl3 KO mESCs. [PDF]
, 2022 (A) The predicted domain structure of proteins encoded by the altered METTL3 ORFs expressed in exon2 Mettl3 KO mESCs suggests they may be functional. The domains that are known to be necessary for m6A formation by METTL3 include the WTAP-binding domain ...
Hui Xian Poh (13118985) +3 more
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Depletion of METTL3 leads to slower proliferation in MEFs. [PDF]
, 2022 (A) Mettl3 conditional knockout MEFs do not express METTL3 protein. To confirm that Mettl3 is the sole m6A writer in cell lines other than mESCs, we produced a tamoxifen-inducible Mettl3 conditional knockout MEF cell line.
Hui Xian Poh (13118985) +3 more
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METTL3 regulates heterochromatin in mouse embryonic stem cells [PDF]
, 2021 METTL3 (methyltransferase-like 3) mediates the N6-methyladenosine (m6A) methylation of mRNA, which affects the stability of mRNA and its translation into protein1.
Wen, J +13 more
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Cry2-mCh-METTL3 undergoes light-inducible LLPS. [PDF]
, 2022 (A) Phase separation of METTL3 and pSC35 in SH-SY5Y cells. (Left) Endogenous METTL3 (green) and the nuclear speckle marker pSC35 (red) were stained using ICC. A layered and punctate distribution of METTL3 was observed surrounding central pSC35 staining. (
Vivian Hoang (12072856) +5 more
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LLPS of Cry2-mCherry-METTL3. [PDF]
, 2022 (A) (Left) Representative confocal images of HEK293T expressing Cry2-mCherry constructs before and after (blue dots) blue light (488 nm) stimulation. (Right) Demixing index at 300 seconds of blue light stimulation was quantified by CV across the nucleus.
Vivian Hoang (12072856) +5 more
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