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Molecular Medicine Today, 1999
Gene targeting has become an extremely popular method for generating mouse models of human disease. The targeting event is carried out in vitro in pluripotent embryonic stem (ES) cells, which are then used to generate chimeric founder animals by either fusion with early-stage embryos or direct injection into donor blastocysts.
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Gene targeting has become an extremely popular method for generating mouse models of human disease. The targeting event is carried out in vitro in pluripotent embryonic stem (ES) cells, which are then used to generate chimeric founder animals by either fusion with early-stage embryos or direct injection into donor blastocysts.
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Insulin Receptor Knockout Mice
Annual Review of Physiology, 2003▪ Abstract To examine the role of the insulin receptor in fuel homeostasis, we and others have carried out genetic ablation studies in mice. Mice lacking insulin receptors are born with normal features, but develop early postnatal diabetes and die of ketoacidosis.
Domenico Accili +2 more
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What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice
, 2000array of choices available for assaying this behavioral construct, and seeing that there are somewhat murky borders between the constructs ‘anxiety’, ‘fear’ and ‘depression’, he or she will explore the question further with an array of tests, after ...
J. Crawley
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Impact of Knockout Mice in Toxicology
Critical Reviews in Toxicology, 1997Knockout mice obtained by homologous recombination technology may be valuable tools for in vivo investigations in toxicopathogenesis. A short review is given on the phenotype of mice with distinct deletions of cytokines and related genes. The application of these mice in pharmacological and toxicological research is discussed, with emphasis in ...
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2003
Interleukin-6 (IL-6) is a pleiotropic cytokine thought to play important regulatory roles in immunity, hemopoiesis, and inflammation (reviewed in ref. 1). The generation of IL-6-deficient mice by gene targeting has allowed direct testing of its role in a variety of physiological and pathological conditions.
POLI, Valeria, MARITANO, DIEGO
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Interleukin-6 (IL-6) is a pleiotropic cytokine thought to play important regulatory roles in immunity, hemopoiesis, and inflammation (reviewed in ref. 1). The generation of IL-6-deficient mice by gene targeting has allowed direct testing of its role in a variety of physiological and pathological conditions.
POLI, Valeria, MARITANO, DIEGO
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Generation of Conditional Knockout Mice
2014Conditional knockout mouse models are powerful tools to examine the biological and molecular function(s) of genes in specific tissues. The general procedure to generate such genetically engineered mouse models consists of three main steps. The first step is to find the appropriate genomic clone of the gene of interest and to design the cloning and ...
Kazuhito Sakamoto +2 more
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Genetic knockouts in mice: An update
Experientia, 1995Gene disruption technology in mammals, by homologous recombination in embryonic stem cells, is a powerful method to manipulate the mouse germ line. In the past decade it has produced a wealth of knowledge concerning neuronal development, neurodegenerative disorders and the roles of oncogenes, Hox genes and growth factors during development.
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Human Molecular Genetics, 2001
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by accumulation of amyloid plaques and neurofibrillary tangles in the brain.
S. Roberds +31 more
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Alzheimer's disease (AD) is a neurodegenerative disorder characterized by accumulation of amyloid plaques and neurofibrillary tangles in the brain.
S. Roberds +31 more
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Annexins – insights from knockout mice
Biological Chemistry, 2016Abstract Annexins are a highly conserved protein family that bind to phospholipids in a calcium (Ca2+) – dependent manner. Studies with purified annexins, as well as overexpression and knockdown approaches identified multiple functions predominantly linked to their dynamic and reversible membrane binding behavior.
Thomas Grewal +3 more
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Bone status of acetylcholinesterase-knockout mice
International Immunopharmacology, 2015Acetylcholinesterase (AChE) hydrolyzes acetylcholine (ACh) to acetate and choline and thereby terminates nerve impulse transmission. ACh is also expressed in bone tissue and enhances here proliferation and differentiation of osteoblasts, which makes it interesting to investigate effects of AChE deficiency on bone.
Mathias Kneffel +9 more
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