Results 11 to 20 of about 397,871 (335)
Isolation of Microtubules and Microtubule Proteins
Current Protocols in Cell Biology, 2008 AbstractThis unit describes various protocols for the isolation and purification of the main constituents of microtubules, chiefly α‐ and β‐tubulin, and the most significant microtubule associated proteins (MAPs), specifically MAP1A, MAP1B, MAP2, and tau.
Jesús Avila +3 more
openaire +3 more sources
Actin–microtubule coordination at growing microtubule ends [PDF]
Nature Communications, 2014 To power dynamic processes in cells, the actin and microtubule cytoskeletons organize into complex structures. Although it is known that cytoskeletal coordination is vital for cell function, the mechanisms by which cross-linking proteins coordinate actin and microtubule activities remain poorly understood.
Preciado López, Magdalena +6 more
openaire +7 more sources
The role of microtubule movement in bidirectional organelle transport [PDF]
, 2008 We study the role of microtubule movement in bidirectional organelle transport in Drosophila S2 cells and show that EGFP-tagged peroxisomes in cells serve as sensitive probes of motor induced, noisy cytoskeletal motions.
A. E. X. Brown +21 more
core +4 more sources
Biochemical reconstitution of branching microtubule nucleation
eLife, 2020 Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins
Raymundo Alfaro-Aco +2 more
doaj +1 more source
Molecules, 2016 While evaluating a large library of compounds designed to inhibit microtubule polymerization, we identified four compounds that have unique effects on microtubules.
Cristina C. Rohena +7 more
doaj +1 more source
Plant Signaling & Behavior, 2023 Plant microtubules (MTs) form highly dynamic and distinct arrays throughout the cell cycle and are essential for cell and organ morphogenesis. A plethora of microtubule associated-proteins (MAPs), both conserved and plant-specific, ensure the dynamic ...
Xiayan Liu, Fei Yu
doaj +1 more source
Tubulin bond energies and microtubule biomechanics determined from nanoindentation in silico [PDF]
, 2015 Microtubules, the primary components of the chromosome segregation machinery, are stabilized by longitudinal and lateral non-covalent bonds between the tubulin subunits.
Ataullakhanov, Fazly I. +7 more
core +1 more source
Neural Regeneration Research, 2015 Cell therapy using stem cell transplantation against cerebral ischemia has been reported. However, it remains controversial regarding the optimal time for cell transplantation and the transplantation route.
Seyed Mojtaba Hosseini +4 more
doaj +1 more source
eLife, 2021 In eukaryotic cells, intracellular components are organized by the microtubule motors cytoplasmic dynein-1 (dynein) and kinesins, which are linked to cargos via adaptor proteins.
Jenna R Christensen +6 more
doaj +1 more source
Cell Motility and the Cytoskeleton, 1999 The regulation of microtubule stability by severing of the polymer along its length is a newly appreciated and potentially important mechanism for controlling microtubule function. Microtubule severing occurs in living cells, but direct observation of this event is infrequent.
L M, Quarmby, T A, Lohret
openaire +2 more sources