Results 41 to 50 of about 227,462 (268)
FEBS Letters, EarlyView.Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley +1 more source
Structural state recognition facilitates tip tracking of EB1 at growing microtubule ends
eLife, 2019 The microtubule binding protein EB1 specifically targets the growing ends of microtubules in cells, where EB1 facilitates the interactions of cellular proteins with microtubule plus-ends.
Taylor A Reid +9 more
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Discerning protein pools by selective staining with self‐labeling tags
FEBS Letters, EarlyView.Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
Structural insight into TPX2-stimulated microtubule assembly
eLife, 2017 During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2.
Rui Zhang +3 more
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Frontiers in Marine Science, 2020 Nuclear filament-containing protein 2 (Nuf2p), a centromere-associated protein, is an indispensable component of Ndc80 complex, which is important for stable microtubule–kinetochore attachment, chromosome pairing, and spindle assembly in mitosis.
Wu-Shan Dong +6 more
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Microtubules born on microtubules
The Journal of Cell Biology, 2005 ![Graphic][1] A new MT is nucleated (white arrowhead) by a γ-TuC (green) that sits on an existing MT bundle.Microtubule (MT) arrays form, say Janson et al. ( [page 297][2]), when MT nucleators stick to existing MTs.
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Molecular Oncology, EarlyView.Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch +13 more
wiley +1 more source
Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis
Molecular Oncology, EarlyView.This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht +3 more
wiley +1 more source
Ca2+ Release by IP3 Receptors Is Required to Orient the Mitotic Spindle
Cell Reports, 2020 Summary: The mitotic spindle distributes chromosomes evenly to daughter cells during mitosis. The orientation of the spindle, guided by internal and external cues, determines the axis of cell division and thereby contributes to tissue morphogenesis ...
Raul Lagos-Cabré +2 more
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The Arabidopsis Book, 2015 Microtubules (MTs) are highly conserved polar polymers that are key elements of the eukaryotic cytoskeleton and are essential for various cell functions. αβ-tubulin, a heterodimer containing one structural GTP and one hydrolysable and exchangeable GTP, is the building block of MTs and is formed by the sequential action of several molecular chaperones ...
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