Structural basis of protein condensation on microtubules underlying branching microtubule nucleation. [PDF]
Nat Commun, 2023 Abstract Targeting protein for Xklp2 (TPX2) is a key factor that stimulates branching microtubule nucleation during cell division. Upon binding to microtubules (MTs), TPX2 forms condensates via liquid-liquid phase separation, which facilitates recruitment of microtubule nucleation factors and tubulin.
Guo C +5 more
europepmc +6 more sources
Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation [PDF]
Nature Communications, 2020 The microtubule binding protein TPX2 enhances branching microtubule nucleation though the current mechanisms are unclear. Here, the authors show that TPX2 undergoes liquid-liquid phase separation and co-condensates with tubulin to enhance TPX2-mediated ...
Matthew R. King, Sabine Petry
doaj +2 more sources
The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. [PDF]
PLoS Biology, 2022 Precise spatiotemporal control of microtubule nucleation and organization is critical for faithful segregation of cytoplasmic and genetic material during cell division and signaling via the primary cilium in quiescent cells.
Umut Batman +2 more
doaj +2 more sources
Small GTPase ARL4C Associated with Various Cancers Affects Microtubule Nucleation [PDF]
BiomedicinesBackground/Objectives: The changes in the level of small GTPase ARL4C are associated with the initiation and progression of many different cancers. The content of ARL4C varies greatly between different tissues, and the induction of ARL4C expression leads
Evgeniia Ulas +2 more
doaj +2 more sources
NEDD1-S411 phosphorylation plays a critical function in the coordination of microtubule nucleation during mitosis. [PDF]
Biol Open, 2022 Timón Pérez K, Scrofani J, Vernos I.
europepmc +2 more sources
Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression. [PDF]
Curr Biol, 2023 Wu J +7 more
europepmc +2 more sources
Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity [PDF]
Nature Communications, 2016 In mature neurons the centrosome no longer functions as the main microtubule organizer and it is unclear how ordered microtubule arrays are assembled. Here, the authors show that in post-mitotic neurons this process depends on non-centrosomal nucleation ...
Carlos Sánchez-Huertas +5 more
doaj +2 more sources
LSPR Biosensing Approach for the Detection of Microtubule Nucleation [PDF]
Sensors, 2019 Microtubules are dynamic protein filaments that are involved in a number of cellular processes. Here, we report the development of a novel localized surface plasmon resonance (LSPR) biosensing approach for investigating one aspect of microtubule dynamics
Keisuke Hasegawa +2 more
doaj +2 more sources
The conserved centrosomin motif, γTuNA, forms a dimer that directly activates microtubule nucleation by the γ-tubulin ring complex (γTuRC). [PDF]
Elife, 2022 Rale MJ +4 more
europepmc +2 more sources
The nucleation of microtubules in Aspergillus nidulans germlings [PDF]
Genetics and Molecular Biology, 1999 Microtubules are filaments composed of dimers of alpha- and beta-tubulins, which have a variety of functions in living cells. In fungi, the spindle pole bodies usually have been considered to be microtubule-organizing centers. We used the antimicrotubule
Cristina de Andrade-Monteiro +1 more
doaj +3 more sources