γ-Tubulin in microtubule nucleation and beyond [PDF]
Frontiers in Cell and Developmental Biology, 2022 Microtubules composed of αβ-tubulin dimers are dynamic cytoskeletal polymers that play key roles in essential cellular processes such as cell division, organelle positioning, intracellular transport, and cell migration.
Vadym Sulimenko +2 more
doaj +6 more sources
C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress [PDF]
Cells, 2022 ER distribution depends on microtubules, and ER homeostasis disturbance activates the unfolded protein response resulting in ER remodeling. CDK5RAP3 (C53) implicated in various signaling pathways interacts with UFM1-protein ligase 1 (UFL1), which ...
Stanislav Vinopal +2 more
exaly +5 more sources
Biochemical reconstitution of branching microtubule nucleation [PDF]
eLife, 2020 Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins
Raymundo Alfaro-Aco +2 more
doaj +6 more sources
CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation [PDF]
eLife, 2022 Microtubules are dynamic polymers consisting of αβ-tubulin heterodimers. The initial polymerization process, called microtubule nucleation, occurs spontaneously via αβ-tubulin.
Tsuyoshi Imasaki +19 more
doaj +3 more sources
Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes in Xenopus laevis egg extract [PDF]
Nature Communications, 2023 Microtubules are generated at centrosomes, chromosomes, and within spindles during cell division. Whereas microtubule nucleation at the centrosome is well characterized, much remains unknown about where, when, and how microtubules are nucleated at ...
Bernardo Gouveia +6 more
doaj +3 more sources
Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG [PDF]
Nature Communications, 2023 The molecular mechanisms underpinning the organization of microtubule arrays remain unclear. Here the authors show that in human cells, the microtubule polymerase ch-TOG promotes nucleation of microtubules at the interphase centrosome and the Golgi ...
Aamir Ali +3 more
doaj +3 more sources
Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression
Current Biology, 2023 Summary Error-free chromosome segregation in mitosis and meiosis relies on the assembly of a microtubule-based spindle that interacts with kinetochores to guide chromosomes to the cell equator before segregation in anaphase.
Sjoerd Klaasen +2 more
exaly +3 more sources
Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation [PDF]
Nature Communications, 2020 The microtubule binding protein TPX2 enhances branching microtubule nucleation though the current mechanisms are unclear. Here, the authors show that TPX2 undergoes liquid-liquid phase separation and co-condensates with tubulin to enhance TPX2-mediated ...
Matthew R. King, Sabine Petry
doaj +3 more sources
Microtubule nucleation without a ring? [PDF]
J Cell Biol, 2021 Merdes previews two studies (Wieczorek et al. and Zimmermann et al.) revealing the γ-TuRC complex structure and elucidating microtubule nucleation mechanisms.
Merdes A.
europepmc +5 more sources
In vitro reconstitution of branching microtubule nucleation. [PDF]
Elife, 2020 Eukaryotic cell division requires the mitotic spindle, a microtubule (MT)-based structure which accurately aligns and segregates duplicated chromosomes.
Tariq A +4 more
europepmc +6 more sources