Results 1 to 10 of about 211,422 (165)

Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation [PDF]

Nature Communications, 2020
The microtubule binding protein TPX2 enhances branching microtubule nucleation though the current mechanisms are unclear. Here, the authors show that TPX2 undergoes liquid-liquid phase separation and co-condensates with tubulin to enhance TPX2-mediated ...
Matthew R King, Sabine Petry
exaly   +4 more sources

Biochemical reconstitution of branching microtubule nucleation [PDF]

eLife, 2020
Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins
Raymundo Alfaro-Aco, Akanksha Thawani, Sabine Petry   +2 more
doaj   +3 more sources

CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation [PDF]

eLife, 2022
Microtubules are dynamic polymers consisting of αβ-tubulin heterodimers. The initial polymerization process, called microtubule nucleation, occurs spontaneously via αβ-tubulin.
Tsuyoshi Imasaki, Satoshi Kikkawa, Shinsuke Niwa, Yumiko Saijo-Hamano, Hideki Shigematsu, Kazuhiro Aoyama, Kaoru Mitsuoka, Takahiro Shimizu, Mari Aoki, Ayako Sakamoto, Yuri Tomabechi, Naoki Sakai, Mikako Shirouzu, Shinya Taguchi, Yosuke Yamagishi, Tomiyoshi Setsu, Yoshiaki Sakihama, Eriko Nitta, Masatoshi Takeichi, Ryo Nitta   +19 more
doaj   +2 more sources

Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes in Xenopus laevis egg extract [PDF]

Nature Communications, 2023
Microtubules are generated at centrosomes, chromosomes, and within spindles during cell division. Whereas microtubule nucleation at the centrosome is well characterized, much remains unknown about where, when, and how microtubules are nucleated at ...
Bernardo Gouveia, Sagar U. Setru, Matthew R. King, Aaron Hamlin, Howard A. Stone, Joshua W. Shaevitz, Sabine Petry   +6 more
doaj   +2 more sources

C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress [PDF]

Cells, 2022
ER distribution depends on microtubules, and ER homeostasis disturbance activates the unfolded protein response resulting in ER remodeling. CDK5RAP3 (C53) implicated in various signaling pathways interacts with UFM1-protein ligase 1 (UFL1), which ...
Anastasiya Klebanovych, Stanislav Vinopal, Eduarda Dráberová, Vladimíra Sládková, Tetyana Sulimenko, Vadym Sulimenko, Věra Vosecká, Libor Macůrek, Agustin Legido, Pavel Dráber   +9 more
doaj   +2 more sources

Molecular insight into microtubule nucleation by the XMAP215/γ-TuRC module [PDF]

Nature Communications
It has become increasingly evident in recent years that nucleation of microtubules from a diverse set of microtubule organizing centers (MTOCs) requires both the γ-tubulin ring complex (γ-TuRC) and the microtubule polymerase XMAP215.
Collin T. McManus, Sophie M. Travis, Philip D. Jeffrey, Rui Zhang, Sabine Petry   +4 more
doaj   +2 more sources

Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG [PDF]

Nature Communications, 2023
The molecular mechanisms underpinning the organization of microtubule arrays remain unclear. Here the authors show that in human cells, the microtubule polymerase ch-TOG promotes nucleation of microtubules at the interphase centrosome and the Golgi ...
Aamir Ali, Chithran Vineethakumari, Cristina Lacasa, Jens Lüders   +3 more
doaj   +2 more sources

Regulation of microtubule nucleation in glioblastoma cells by ARF GTPase-activating proteins GIT1 and GIT2 and protein kinase C [PDF]

Cancer Cell International
Background G protein-coupled receptor kinase-interacting proteins (GITs) function as GTPase-activating proteins (GAPs) for small GTPases of the ADP-ribosylation factor (Arf) family.
Vadym Sulimenko, Eduarda Dráberová, Vladimíra Sládková, Tetyana Sulimenko, Věra Vosecká, Omar Skalli, Pavel Dráber   +6 more
doaj   +2 more sources

γ-Tubulin in microtubule nucleation and beyond [PDF]

Frontiers in Cell and Developmental Biology, 2022
Microtubules composed of αβ-tubulin dimers are dynamic cytoskeletal polymers that play key roles in essential cellular processes such as cell division, organelle positioning, intracellular transport, and cell migration.
Vadym Sulimenko, Eduarda Dráberová, Pavel Dráber   +2 more
doaj   +2 more sources

Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2 [PDF]

Frontiers in Immunology
Aggregation of high-affinity IgE receptors (FcϵRIs) on granulated mast cells triggers signaling pathways leading to a calcium response and release of inflammatory mediators from secretory granules.
Vadym Sulimenko, Vladimíra Sládková, Tetyana Sulimenko, Eduarda Dráberová, Věra Vosecká, Lubica Dráberová, Omar Skalli, Pavel Dráber   +7 more
doaj   +2 more sources