Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression [PDF]
Current Biology, 2023 Error-free chromosome segregation in mitosis and meiosis relies on the assembly of a microtubule-based spindle that interacts with kinetochores to guide chromosomes to the cell equator before segregation in anaphase.
Sjoerd J Klaasen +2 more
exaly +5 more sources
Quantitative Plant BiologyThe development of the water transporting xylem tissue in plants involves an intricate interplay of Rho-of-Plants (ROP) proteins and cortical microtubules to generate highly functional secondary cell wall patterns, such as the ringed or spiral patterns ...
Bas Jacobs +4 more
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S. pombe kinesins-8 promote both nucleation and catastrophe of microtubules. [PDF]
PLoS ONE, 2012 The kinesins-8 were originally thought to be microtubule depolymerases, but are now emerging as more versatile catalysts of microtubule dynamics. We show here that S.
Muriel Erent +2 more
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Structural basis of human γTuRC closure during CM1-activated microtubule nucleation [PDF]
Nature CommunicationsMicrotubule nucleation by the γ-tubulin ring complex (γTuRC) is spatiotemporally regulated and in higher eukaryotes is thought to involve a transition from an inactive open to an active closed conformation that matches the microtubule geometry.
Marina Serna +9 more
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The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. [PDF]
PLoS Biology, 2022 Precise spatiotemporal control of microtubule nucleation and organization is critical for faithful segregation of cytoplasmic and genetic material during cell division and signaling via the primary cilium in quiescent cells.
Umut Batman +2 more
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Endocytosis of Wnt ligands from surrounding epithelial cells positions microtubule nucleation sites at dendrite branch points. [PDF]
PLoS BiologyMicrotubule nucleation is important for microtubule organization in dendrites and for neuronal injury responses. The core nucleation protein, γTubulin (γTub), is localized to dendrite branch points in Drosophila sensory neurons by Wnt receptors and ...
Pankajam Thyagarajan +7 more
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Microtubule Nucleation Properties of Single Human γTuRCs Explained by Their Cryo-EM Structure [PDF]
Developmental Cell, 2020 The γ-tubulin ring complex (γTuRC) is the major microtubule nucleator in cells. The mechanism of its regulation is not understood. We purified human γTuRC and measured its nucleation properties in a total internal reflection fluorescence (TIRF ...
Juri Rappsilber +2 more
exaly +4 more sources
Small GTPase ARL4C Associated with Various Cancers Affects Microtubule Nucleation [PDF]
BiomedicinesBackground/Objectives: The changes in the level of small GTPase ARL4C are associated with the initiation and progression of many different cancers. The content of ARL4C varies greatly between different tissues, and the induction of ARL4C expression leads
Evgeniia Ulas +2 more
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LSPR Biosensing Approach for the Detection of Microtubule Nucleation [PDF]
Sensors, 2019 Microtubules are dynamic protein filaments that are involved in a number of cellular processes. Here, we report the development of a novel localized surface plasmon resonance (LSPR) biosensing approach for investigating one aspect of microtubule dynamics
Keisuke Hasegawa +2 more
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Cell edges accumulate gamma tubulin complex components and nucleate microtubules following cytokinesis in Arabidopsis thaliana. [PDF]
PLoS ONE, 2011 Microtubules emanate from distinct organizing centers in fungal and animal cells. In plant cells, by contrast, microtubules initiate from dispersed sites in the cell cortex, where they then self-organize into parallel arrays.
Chris Ambrose, Geoffrey O Wasteneys
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