Microtubule Nucleation Properties of Single Human γTuRCs Explained by Their Cryo-EM Structure
Developmental Cell, 2020 Summary The γ-tubulin ring complex (γTuRC) is the major microtubule nucleator in cells. The mechanism of its regulation is not understood. We purified human γTuRC and measured its nucleation properties in a total internal reflection fluorescence (TIRF ...
Juri Rappsilber +2 more
exaly +3 more sources
Microtubule nucleation for the assembly of acentrosomal microtubule arrays in plant cells [PDF]
New Phytologist, 2019 Contents Summary I. Introduction II. MT arrays in plant cells III. γ-Tubulin and MT nucleation IV. MT nucleation sites or flexible MTOCs in plant cells V. MT-dependent MT nucleation VI. Generating new MTs for spindle assembly VII.
Yuh-Ru Julie Lee, Bo Liu
exaly +4 more sources
Cells, 2019 The antigen-mediated activation of mast cells initiates signaling events leading to their degranulation, to the release of inflammatory mediators, and to the synthesis of cytokines and chemokines.
Pavel Draber +2 more
exaly +4 more sources
LSPR Biosensing Approach for the Detection of Microtubule Nucleation [PDF]
Sensors, 2019 Microtubules are dynamic protein filaments that are involved in a number of cellular processes. Here, we report the development of a novel localized surface plasmon resonance (LSPR) biosensing approach for investigating one aspect of microtubule dynamics
Keisuke Hasegawa
exaly +4 more sources
Structural basis of protein condensation on microtubules underlying branching microtubule nucleation. [PDF]
Nat Commun, 2023 Targeting protein for Xklp2 (TPX2) is a key factor that stimulates branching microtubule nucleation during cell division. Upon binding to microtubules (MTs), TPX2 forms condensates via liquid-liquid phase separation, which facilitates recruitment of ...
Guo C +5 more
europepmc +2 more sources
Microtubule nucleation for spindle assembly: one molecule at a time. [PDF]
Trends Biochem Sci, 2023 The cell orchestrates the dance of chromosome segregation with remarkable speed and fidelity. The mitotic spindle is built from scratch after interphase through microtubule (MT) nucleation, which is dependent on the γ-tubulin ring complex (γ-TuRC), the ...
Kraus J +3 more
europepmc +2 more sources
Microtubule nucleation complex behavior is critical for cortical array homogeneity and xylem wall patterning. [PDF]
Proc Natl Acad Sci U S A, 2022 Significance The plant cortical microtubule array is an established model system for self-organization, with a rich history of complementary experiments, computer simulations, and analytical theory.
Jacobs B +4 more
europepmc +2 more sources
Nature Reviews Molecular Cell Biology Microtubule nucleation by γ-tubulin complexes [PDF]
Nature Reviews Molecular Cell Biology, 2011 Microtubule nucleation is regulated by the γ-tubulin ring complex (γTuRC) and related γ-tubulin complexes, providing spatial and temporal control over the initiation of microtubule growth.
J. Kollman +3 more
semanticscholar +3 more sources
GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation. [PDF]
J Cell Biol, 2021 Ayukawa, Iwata, Imai, et al. visualize the early intermediates in the pathway of spontaneous nucleation of microtubules by using rapid flush negative stain electron microscopy.
Ayukawa R +13 more
europepmc +2 more sources
Autoinhibition of Cnn binding to γ-TuRCs prevents ectopic microtubule nucleation and cell division defects. [PDF]
J Cell Biol, 2021 We show that autoinhibition regulates the binding between microtubule nucleating complexes and proteins that tether them to sites of microtubule nucleation.
Tovey CA +6 more
europepmc +2 more sources