Results 31 to 40 of about 350,705 (311)

Affinity chromatography purification of mitochondrial inner membrane proteins with calcium transport activity

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1998
Immobilized calcium affinity chromatography was used to obtain a preparation enriched in calcium transporters from Triton X-100 extracts of rat liver mitochondria inner membranes (PPCT). The PPCT were reconstituted into preformed asolectin liposomes which contained 120 mM KCl as internal high K+ medium.
Villa, Ana, García-Simón, Maria Isabel, Blanco, Pablo, Sesé, Bárbara, Bogónez, Elena, Satrustegui, Jorgina   +5 more
openaire   +2 more sources

NMR and Patch-Clamp Characterization of Yeast Mitochondrial Pyruvate Carrier Complexes

Biomolecules, 2023
The mitochondrial pyruvate carrier (Mpc) plays an indispensable role in the transport of pyruvates across the mitochondrial inner membrane. Despite the two distinct homologous proteins, Mpc1 and Mpc2, were identified in 2012, there are still ...
Zhen Wang, Wen Ding, Maosen Ruan, Yong Liu, Jing Yang, Huiqin Zhang, Bing Shen, Junfeng Wang, Yunyan Li   +8 more
doaj   +1 more source

Developmental changes in trak-mediated mitochondrial transport in neurons [PDF]

, 2017
Previous studies established that the kinesin adaptor proteins, TRAK1 and TRAK2, play an important role in mitochondrial transport in neurons. They link mitochondria to kinesin motor proteins via a TRAK acceptor protein in the mitochondrial outer ...
Loss, O, Stephenson, FA
core   +1 more source

Redox-Mediated Regulation of Mitochondrial Biogenesis, Dynamics, and Respiratory Chain Assembly in Yeast and Human Cells

Frontiers in Cell and Developmental Biology, 2021
Mitochondria are double-membrane organelles that contain their own genome, the mitochondrial DNA (mtDNA), and reminiscent of its endosymbiotic origin.
Stefan Geldon, Erika Fernández-Vizarra, Kostas Tokatlidis   +2 more
doaj   +1 more source

Successive translocation into and out of the mitochondrial matrix [PDF]

, 1987
We investigated the import and sorting pathways of cytochrome b2 and cytochrome c1, which are functionally located in the intermembrane space of mitochondria.
Alt, Appleby, Bradford, Böhni, Chen, Conboy, Daum, Dowhan, Duffaud, Eilers, Fujiki, Gabellini, Gabellini, Gasser, Gasser, Gubler, Guiard, Hallermayer, Harmey, Hartl, Hartl, Hartl, Hauska, Hay, Hayashi, Horwich, Hurt, Hurt, Hurt, Kaput, Kolanski, Krieg, Laemmli, Lewin, Li, McAda, Miura, Ohashi, Pelham, Perara, Pfanner, Pfanner, Pfanner, Pfanner, Pfanner, Pfanner, Pratje, Riezmann, Rottier, Römisch, Sadler, Schatz, Schleyer, Schleyer, Schmidt, Schmidt, Schwaiger, Simon, Smeekens, Stueber, Talmadge, Teintze, van Loon, van Loon, van Loon, Voordouw, Zimmermann, Zwizinski   +67 more
core   +1 more source

Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability

eLife, 2016
The TIM22 complex mediates the import of hydrophobic carrier proteins into the mitochondrial inner membrane. While the TIM22 machinery has been well characterised in yeast, the human complex remains poorly characterised. Here, we identify Tim29 (C19orf52)
Yilin Kang, Michael James Baker, Michael Liem, Jade Louber, Matthew McKenzie, Ishara Atukorala, Ching-Seng Ang, Shivakumar Keerthikumar, Suresh Mathivanan, Diana Stojanovski   +9 more
doaj   +1 more source

Mitochondrial Quality Control Governed by Ubiquitin

Frontiers in Cell and Developmental Biology, 2020
Mitochondria are essential organelles important for energy production, proliferation, and cell death. Biogenesis, homeostasis, and degradation of this organelle are tightly controlled to match cellular needs and counteract chronic stress conditions ...
Sonia Ravanelli, Fabian den Brave, Thorsten Hoppe, Thorsten Hoppe   +3 more
doaj   +1 more source

Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view [PDF]

, 2006
The proper functioning of mitochondria requires that both the mitochondrial and the nuclear genome are functional. To investigate the importance of the mitochondrial genome, which encodes only 13 subunits of the respiratory complexes, the mitochondrial ...
Abdul, Appleby, Armstrong, Bai, Bourges, Buchet, Chai, Claros, Da Cruz, Dey, Du, Garcia, Gianazza, Joseph, King, King, Lescuyer, Luche, Manjasetty, Martinus, Perkins, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Santoni, Sharma, Sharp, Sherer, Singh, Sinha, Suzuki, Tastet, Taylor, Tryoen-Toth, Ugalde, Wiedemann, Zamzami, Zischka   +39 more
core   +4 more sources

Early steps in steroidogenesis: intracellular cholesterol trafficking

Journal of Lipid Research, 2011
Steroid hormones are made from cholesterol, primarily derived from lipoproteins that enter cells via receptor-mediated endocytosis. In endo-lysosomes, cholesterol is released from cholesterol esters by lysosomal acid lipase (LAL; disordered in Wolman ...
Walter L. Miller, Himangshu S. Bose
doaj   +1 more source

Palmitic Acid, but Not Lauric Acid, Induces Metabolic Inflammation, Mitochondrial Fragmentation, and a Drop in Mitochondrial Membrane Potential in Human Primary Myotubes

Frontiers in Nutrition, 2021
The chain length of saturated fatty acids may dictate their impact on inflammation and mitochondrial dysfunction, two pivotal players in the pathogenesis of insulin resistance.
Domenico Sergi, Domenico Sergi, Natalie Luscombe-Marsh, Nenad Naumovski, Nenad Naumovski, Mahinda Abeywardena, Nathan O'Callaghan   +6 more
doaj   +1 more source