Results 71 to 80 of about 491,817 (253)

Dose response of endotoxin on hepatocyte and muscle mitochondrial respiration in vitro. [PDF]

, 2015
INTRODUCTION Results on mitochondrial dysfunction in sepsis are controversial. We aimed to assess effects of LPS at wide dose and time ranges on hepatocytes and isolated skeletal muscle mitochondria. METHODS Human hepatocellular carcinoma cells (
Stephan M. Jakob, Jakob, Stephan, Siamak Djafarzadeh, Brandt, Sebastian, Jukka Takala, Djafarzadeh, Siamak, Sebastian Brandt, Porta, Francesca, Takala, Jukka, Victor Jeger, Jeger, Victor, Francesca Porta   +11 more
core   +2 more sources

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Quantifying variability associated with high-resolution respirometry in human permeabilized skeletal muscle fibers

Advanced Exercise and Health Science
Rates of skeletal muscle mitochondrial respiration are often measured using an Oroboros Oxygraph-2K (O2K) high-resolution respirometer. The purpose of this investigation was to determine the coefficients of variation (CV) and technical error of ...
Emily J. Ferguson, Justin Bureau, Tanner Stokes, Danielle Nyman, Joel Seigel, Shivam Gandhi, Luca J. Delfinis, Brendon J. Gurd, Christopher G.R. Perry, Chris McGlory   +9 more
doaj   +1 more source

Palmitate-Induced Cell Death and Mitochondrial Respiratory Dysfunction in Myoblasts are Not Prevented by Mitochondria-Targeted Antioxidants

Cellular Physiology and Biochemistry, 2014
Background/Aims: Deleterious effects of saturated fatty acids in skeletal muscle cells are well known but their impact on mitochondrial respiration has not been well studied.
Jana Patková, Michal Anděl, Jan Trnka
doaj   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

The Effects of Thiocyanate on Mitochondrial Respiration

Experimental Biology and Medicine, 1968
The thiocyanate ion, SCN-, has been extensively employed as a reversible inhibitor of gastric acid secretion. We have reported cytochrome redox changes associated with SCN inhibition in bullfrog gastric mucosa 1 and, since we found no SCN- inhibition of oxygen consumption in tissue mince, concluded that the primary action of SCN- was on the acid ...
openaire   +2 more sources

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Aging Is a Key Driver for Adult Acute Myeloid Leukemia

Aging and Cancer, EarlyView.
Acute myeloid leukemia (AML) is a classical age‐related hematologic malignancy, and a key driver of AML is aging, which profoundly regulates intrinsic factors such as genomic instability, epigenetic reprogramming, and metabolic dysregulation, and alters bone marrow microenvironment.
Rong Yin, Haojian Zhang
wiley   +1 more source

High‐fat diet increases electron transfer flavoprotein synthesis and lipid respiration in skeletal muscle during exercise training in female mice

Physiological Reports, 2023
High‐fat diet (HFD) and exercise remodel skeletal muscle mitochondria. The electron transfer flavoproteins (ETF) transfer reducing equivalents from β‐oxidation into the electron transfer system.
Philip M. Batterson, Erin M. McGowan, Agnieszka K. Borowik, Michael T. Kinter, Benjamin F. Miller, Sean A. Newsom, Matthew M. Robinson   +6 more
doaj   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source