Results 101 to 110 of about 1,257,185 (207)

mRNA Cap Methyltransferase, RNMT-RAM, Promotes RNA Pol II-Dependent Transcription

Cell Reports, 2018
Summary: mRNA cap addition occurs early during RNA Pol II-dependent transcription, facilitating pre-mRNA processing and translation. We report that the mammalian mRNA cap methyltransferase, RNMT-RAM, promotes RNA Pol II transcription independent of mRNA ...
Dhaval Varshney, Olivia Lombardi, Gabriele Schweikert, Sianadh Dunn, Olga Suska, Victoria H. Cowling   +5 more
doaj   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

The effect of RNA base lesions on mRNA translation [PDF]

, 2015
The biological effect of oxidatively damaged RNA, unlike oxidatively damaged DNA, has rarely been investigated, although it poses a threat to any living cell. Here we report on the effect of the commonly known RNA base-lesions 8-oxo-rG, 8-oxo-rA, ε-rC, ε-
Leumann, Christian J., Küpfer, Pascal A., Calabretta, Alessandro   +2 more
core   +2 more sources

Mechanism of trans-translation revealed by in vitro studies

Frontiers in Microbiology, 2014
tmRNA is a bacterial small RNA having a structure resembling the upper half of tRNA and its 3’ end accepts alanine followed by binding to EF-Tu like tRNA.
Hyouta eHimeno, Hyouta eHimeno, Daisuke eKurita, Akira eMuto   +3 more
doaj   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Overlapping signals for translational regulation and packaging of influenza A virus segment 2 [PDF]

, 2011
Influenza A virus segment 2 mRNA expresses three polypeptides: PB1, PB1-F2 and PB1-N40, from AUGs 1, 4 and 5 respectively. Two short open reading frames (sORFs) initiated by AUGs 2 and 3 are also present.
J. K. Taubenberger, Taubenberger, J.K., Digard, P., McCarthy, John E G, Edmund T. Rolls, J.R. Gog, Wise, Helen M., Amy J. Claydon, John E. G. Mccarthy, M. D. Curran, J.K. Taubenberger, H. M. Wise, Eyers, Claire, Eyers, Claire E., Claydon, AJ, P. Digard, Beynon, RJ, Deco Gustavo, McCarthy, JEG, Dalton, Rosa Maria, Kannambath, Shichina, E.C. Anderson, Rolls, Edmund T, Tristan J. Webb, Helen M. Wise, B. W. Jagger, Julia R. Gog, E. C. Anderson, Curran, M.D., Paul Digard, B.W. Jagger, Jagger, Brett W., H.M. Wise, R.M. Dalton, Claydon, Amy J., Anderson, E.C., Jeffery K. Taubenberger, Li, N, Tristan J Webb, Drummond, Sheona P., Deco, Gustavo, Hildyard, John, Webb, Tristan J., Edmund T Rolls, Gustavo Deco, Reamtong, O, Eyers, CE, Jagger, B.W., Helena Firczuk, C. Barbezange, Drummond, SP, Curran, Martin D., Jianfeng Feng, Robert J. Beynon, Webb Tristan J., John Hildyard, Taubenberger, Jeffery, Rolls, Edmund T., J. R. Gog, Feng Jianfeng, Firczuk, H, Sheona P. Drummond, Cyril Barbezange, Li, Ning, Onrapak Reamtong, Wise, H.M., Brett W. Jagger, Kannambath, S, Digard, Paul, Gog, Julia R., Gog, J.R., Anderson, Emma C., McCarthy, John E. G., Martin D. Curran, Shichina Kannambath, R. M. Dalton, Rosa M. Dalton, Barbezange, Cyril, Hildyard, J, Rolls Edmund T., Feng, Jianfeng, Barbezange, C., Dalton, R.M., Beynon, Robert J., M.D. Curran, Reamtong, Onrapak, Beynon, R. J. (Robert J.), Ning Li, Claire E. Eyers, Emma C. Anderson, Firczuk, Helena   +90 more
core   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

MARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA

Nature Communications
The mammalian early embryo development requires translation of maternal mRNA inherited from the oocyte. While poly(A) tail length influences mRNA translation efficiency during the oocyte-to-embryo transition (OET), molecular mechanisms regulating ...
Jing Yang, Jiachen Bu, Bowen Liu, Yusheng Liu, Zhuqiang Zhang, Ziyi Li, Falong Lu, Bing Zhu, Yingfeng Li   +8 more
doaj   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source