Results 51 to 60 of about 1,562 (148)
mBio, 2014 The Escherichia coli structural maintenance of chromosome (SMC) complex, MukBEF, and topoisomerase IV (TopoIV) interact in vitro through a direct contact between the MukB dimerization hinge and the C-terminal domain of ParC, the catalytic subunit of ...
Emilien Nicolas +5 more
doaj +1 more source
High-resolution definition of the Vibrio cholerae essential gene set with hidden Markov model–based analyses of transposon-insertion sequencing data [PDF]
, 2014 The coupling of high-density transposon mutagenesis to high-throughput DNA sequencing (transposon-insertion sequencing) enables simultaneous and genome-wide assessment of the contributions of individual loci to bacterial growth and survival.
Chao, Michael C. +6 more
core +1 more source
An integrative, multi-scale, genome-wide model reveals the phenotypic landscape of Escherichia coli. [PDF]
, 2014 Given the vast behavioral repertoire and biological complexity of even the simplest organisms, accurately predicting phenotypes in novel environments and unveiling their biological organization is a challenging endeavor.
Carrera, Javier +5 more
core +1 more source
Dissecting DNA Compaction by the Bacterial Condensin MukB
, 2019 Condensins in bacteria are one of the most important factors involved in the organization of long threads of DNA into compact chromosomes. The organization of DNA by condensins is vital to many DNA transactions including DNA repair and chromosome segregation.
Rupesh, Kumar +2 more
openaire +3 more sources
Role of the mukB gene in chromosome and plasmid partition in Escherichia coli [PDF]
Molecular Microbiology, 2000 The intracellular locations of oriC and oriR1, the replication origins of the chromosome and plasmid R1, respectively, were visualized by fluorescence in situ hybridization (FISH) in exponentially growing populations of Escherichia coli. The locations of oriC and oriR1 (from a Par+ R1 plasmid) were unique and different in the wild‐type host.
T, Weitao, S, Dasgupta, K, Nordström
openaire +2 more sources
Crystal structure of the N-terminal domain of MukB: a protein involved in chromosome partitioning [PDF]
Structure, 1999 The 170 kDa protein MukB has been implicated in ATP-dependent chromosome partitioning during cell division in Escherichia coli. MukB shares its dimeric structure and domain architecture with the ubiquitous family of SMC (structural maintenance of chromosomes) proteins that facilitate similar functions.
van den Ent, Fusinita +3 more
openaire +2 more sources
Tuned SMC Arms Drive Chromosomal Loading of Prokaryotic Condensin. [PDF]
, 2017 SMC proteins support vital cellular processes in all domains of life by organizing chromosomal DNA. They are composed of ATPase "head" and "hinge" dimerization domains and a connecting coiled-coil "arm." Binding to a kleisin subunit creates a closed ...
Basfeld, A. +5 more
core +2 more sources
Dynamic distribution of SeqA protein across the chromosome of Escherichia coli K-12 [PDF]
, 2010 The bacterial SeqA protein binds to hemi-methylated GATC sequences that arise in newly synthesized DNA upon passage of the replication machinery. In Escherichia coli K-12, the single replication origin oriC is a well-characterized target for SeqA, which ...
Bach +48 more
core +2 more sources
Dynamic molecular linkers of the genome: the first decade of SMC proteins [PDF]
, 2005 Structural maintenance of chromosomes (SMC) proteins are chromosomal ATPases, highly conserved from bacteria to humans, that play fundamental roles in many aspects of higher-order chromosome organization and dynamics.
Hirano, T., Losada, A.
core +1 more source
Microorganisms, 2019 Prokaryotes have an essential gene—gyrase—that catalyzes negative supercoiling of plasmid and chromosomal DNA. Negative supercoils influence DNA replication, transcription, homologous recombination, site-specific recombination, genetic ...
Nikolay S. Rovinskiy +3 more
doaj +1 more source