Results 181 to 190 of about 465,638 (353)

OGFRL1 deficiency causes CRMO via pathological osteoclastogenesis, with therapeutic response to TNF inhibitor

Arthritis &Rheumatology, Accepted Article.
Objectives To verify the pathogenesis of the OGFRL1 loss‐of‐function variant (c.30del, p. F10Ffs*110) identified in a CRMO patient and investigate the underlying mechanism. Methods Whole exome sequencing and Sanger sequencing were performed to identify and confirm the variant.
Wen Xiong, Yusha Wang, Tingyan He, Jinjian Fu, Ying Luo, Jinbo Wang, Jun Wang, Xiaomin Yu, Qing Zhou, Jun Yang   +9 more
wiley   +1 more source

Improvement of EPO Transgene Detection From Polymeric Dried Blood Spots for Antidoping Application

Drug Testing and Analysis, EarlyView.
This article presents an optimized protocol for gene doping detection. Starting from one spot DBS (20 μL), concentrated DNA was obtained and real‐time PCR parameters were adjusted for increased detection. Robust detection of 5000 copies/mL of transgene was validated for polymeric device TASSO M‐20.
Alexandre Marchand, Ingrid Roulland, Magnus Ericsson   +2 more
wiley   +1 more source

Multiplex Polymerase Chain Reaction Panels for Gastrointestinal Infections: Current Evidence, Regulatory Hurdles, and the Way Forward. [PDF]

Open Forum Infect Dis
Tansarli GS, Allen DR, Fang FC.
europepmc   +1 more source

Rapid identification of bacteria using a multiplex polymerase chain reaction system for acute abdominal infections. [PDF]

Front Microbiol, 2023
Kakizaki N, Asai K, Kuroda M, Watanabe R, Kujiraoka M, Sekizuka T, Katagiri M, Moriyama H, Watanabe M, Saida Y.   +9 more
europepmc   +1 more source

Combinatorial expression of glial transcription factors induces Schwann cell‐specific gene expression in mouse embryonic fibroblasts

Developmental Dynamics, EarlyView.
Abstract Background Schwann cells provide peripheral nerve trophic support, myelinate axons, and assist in repair. However, Schwann cell repair capacity is limited by chronic injury, disease, and aging. Schwann cell reprogramming is a cellular conversion strategy that could provide a renewable cell supply to repair injured nerves.
Lauren Belfiore, Anjali Balakrishnan, Yacine Touahri, Dawn Zinyk, Humna Noman, Satoshi Okawa, Jeff Biernaskie, Carol Schuurmans   +7 more
wiley   +1 more source

Multiplex Polymerase Chain Reaction (PCR) and Conventional Methods for Diagnosing Ventilator-Associated Pneumonia in ICU Settings: A Systematic Review. [PDF]

Cureus
Fadel Yosif AS, Eltayeb Blado GK, Bashir Eltayeb MA, Shamina NA, Ahmed Abdalaziz MA, Osman A.   +5 more
europepmc   +1 more source

Implementation of a Rapid Multiplex Polymerase Chain Reaction Pneumonia Panel and Subsequent Antibiotic De-escalation. [PDF]

Open Forum Infect Dis, 2023
Miller MM, Van Schooneveld TC, Stohs EJ, Marcelin JR, Alexander BT, Watkins AB, Creager HM, Bergman SJ.   +7 more
europepmc   +1 more source

Multiplex polymerase chain reaction for identification of Escherichia coli, Escherichia albertii and Escherichia fergusonii.

Journal of Microbiological Methods, 2017
R. Lindsey, Lisley Garcia-Toledo, Lisley Garcia-Toledo, D. Fasulo, Lori M. Gladney, N. Strockbine   +5 more
semanticscholar   +1 more source

Outcome of IWGT workshop on transcriptomic biomarkers for genotoxicity: Key considerations for bioinformatics

Environmental and Molecular Mutagenesis, EarlyView.
Abstract As a part of the International Workshop on Genotoxicity Testing (IWGT) in 2022, a workgroup was formed to evaluate the level of validation and regulatory acceptance of transcriptomic biomarkers that identify genotoxic substances. Several such biomarkers have been developed using various molecular techniques and computational approaches. Within
Matthew J. Meier, Florian Caiment, J. Christopher Corton, Roland Frötschl, Yurika Fujita, Danyel Jennen, Roman Mezencev, Carole Yauk   +7 more
wiley   +1 more source

Detection of Acute Tropical Fever Pathogens via Multiplex Polymerase Chain Reaction: A Cross-Sectional Study to Guide Appropriate Antimicrobial Use in Communities. [PDF]

Cureus
Ahmed R, Irfan S, Baig A, Ghanchi N, Qamar FN, Zafar A.   +5 more
europepmc   +1 more source