Results 31 to 40 of about 465,638 (353)

Specific detection of Salmonella spp. by multiplex polymerase chain reaction [PDF]

Applied and Environmental Microbiology, 1993
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria.
J S, Way, K L, Josephson, S D, Pillai, M, Abbaszadegan, C P, Gerba, I L, Pepper   +5 more
openaire   +2 more sources

Detection of Microsatellite Instability by Fluorescence Multiplex Polymerase Chain Reaction [PDF]

The Journal of Molecular Diagnostics, 2000
We have created a clinical molecular diagnostic assay to test for microsatellite instability (MSI) at multiple loci simultaneously in paraffin-embedded surgical pathology colon resection specimens. This fluorescent multiplex polymerase chain reaction (PCR) assay analyzes the five primary microsatellite loci recommended at the 1997 National Cancer ...
K D, Berg, C L, Glaser, R E, Thompson, S R, Hamilton, C A, Griffin, J R, Eshleman   +5 more
openaire   +2 more sources

An Economic Modeling Study of Helicobacter pylori Eradication: Comparison of Dual Priming Oligonucleotide-Based Multiplex Polymerase Chain Reaction and Empirical Treatment

Gut and Liver, 2018
Background/Aims Dual priming oligonucleotide-based multiplex polymerase chain reaction (DPO-based PCR) can detect the presence of clarithromycin resistance without culture.
T. Gweon, J. Kim, Byung-Wook Kim
semanticscholar   +1 more source

NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions [PDF]

PLOS Computational Biology, 2020
Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of amplicon-based genome target ...
Andrey Kechin, Viktoria Borobova, Ulyana Boyarskikh, Evgeniy Khrapov, Sergey Subbotin, Maxim Filipenko   +5 more
openaire   +4 more sources

Detection of common diarrhea-causing pathogens in Northern Taiwan by multiplex polymerase chain reaction

Medicine, 2018
Conventional methods for identifying gastroenteritis pathogens are time consuming, more likely to result in a false-negative, rely on personnel with diagnostic expertise, and are dependent on the specimen status.
Shu-huan Huang, Yi-Fang Lin, M. Tsai, Shuan Yang, Mei-Ling Liao, Shao-Wen Chao, Cheng-cheng Hwang   +6 more
semanticscholar   +1 more source

Development and evaluation of a multiplex droplet digital polymerase chain reaction method for simultaneous detection of five biothreat pathogens

Frontiers in Microbiology, 2022
Biothreat agents pose a huge threat to human and public health, necessitating the development of rapid and highly sensitive detection approaches.
Yipu Du, Ziheng Yan, Kai Song, Junyan Jin, Liting Xiao, Zhulin Sun, Ya-fang Tan, Pingping Zhang, Z. Du, Ruifu Yang, Yong Zhao, Yajun Song   +11 more
semanticscholar   +1 more source

An evaluation of serotyping of Avibacterium paragallinarum by use of a multiplex polymerase chain reaction [PDF]

Journal of Veterinary Diagnostic Investigation, 2014
Vladimir Morales-Erasto, José de Jesús Posadas-Quintana, Manolo Fernández‐Díaz, Luis E. Saravia, José Simón Martínez-Castañeda, P. J. Blackall, Edgardo Soriano‐Vargas   +6 more
openalex   +2 more sources

Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms

Data in Brief, 2015
This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain ...
Makiko Shimizu, Tomoki Koyama, Izumi Kishimoto, Hiroshi Yamazaki   +3 more
doaj   +1 more source

Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction

Veterinary World, 2017
Aim: The objective of the study was to investigate the occurrence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using the multiplex polymerase chain reaction (PCR)
C. Latha, C. Anu, V. Ajaykumar, B. Sunil
semanticscholar   +1 more source

Detection of bovine pestiviruses by a multiplex real-time polymerase chain reaction

Вопросы вирусологии, 2020
Introduction. Pestiviruses are the cause of reproductive problems, diseases of the gastrointestinal and respiratory tracts of animals. Three species are important for cattle: Pestivirus A, B, and H.
Aleksey V. Nefedchenko, Svetlana V. Koteneva, Tatyana I. Glotova, Alexander G. Glotov   +3 more
doaj   +1 more source