Results 31 to 40 of about 129,024 (210)

Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs [PDF]

, 1999
Detection of respiratory viruses by realtime multiplexed PCR (M-PCR) and of RSV by M-PCR and immunofluorescence(IF) was evaluated using specimens collected by nasopharyngeal flocked swab(NFS) and nasal wash(NW).
Munywoki, Patrick, Hamid, Fauzat, Mutunga, Martin, Welch, Steve, Cane, Patricia, Nokes, D. James   +5 more
core   +2 more sources

Validation and implementation of a diagnostic algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B-holmesii in a Pediatric Referral Hospital in Barcelona, Spain [PDF]

, 2019
This study aimed to validate a comprehensive diagnostic protocolbased on real-time PCR for the rapid detection and identification ofBordetella per-tussis,Bordetella parapertussis, andBordetella holmesii, as well as its implementationin the diagnostic ...
Acosta Argueta, Lesly María, Godoy Garcia, Pere, Henares Bonilla, Desirée, Jané Checa, Mireia, Muñoz Almagro, Carmen, Valero Rello, Anna   +5 more
core   +2 more sources

Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms

Data in Brief, 2015
This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain ...
Makiko Shimizu, Tomoki Koyama, Izumi Kishimoto, Hiroshi Yamazaki   +3 more
doaj   +1 more source

Multiplex PCR assay for the simultaneous detection of bacterial pathogens in rainbow trout [PDF]

, 2017
Accurate, rapid, and specific methods are required to identify pathogens for controlling bacterial diseases in fish. Various microbiological and biochemical methods are used to isolate and identify pathogens, but they are not adequately efficient and/or ...
Naeemipour, Mohsen, Rajabzadeh, Nadia, Seyedabadi, Mohsen   +2 more
core   +1 more source

Detection of bovine pestiviruses by a multiplex real-time polymerase chain reaction

Вопросы вирусологии, 2020
Introduction. Pestiviruses are the cause of reproductive problems, diseases of the gastrointestinal and respiratory tracts of animals. Three species are important for cattle: Pestivirus A, B, and H.
Aleksey V. Nefedchenko, Svetlana V. Koteneva, Tatyana I. Glotova, Alexander G. Glotov   +3 more
doaj   +1 more source

Forensic validation studies of a novel 35-InDel multiplex polymerase chain reaction system

Journal of Forensic Science and Medicine, 2023
Background: A difficulty associated with forensic applications is the detection of degraded biological materials. Due to the large amplicon sizes of short tandem repeat alleles, valid genotyping results cannot be obtained from degraded biological ...
Tong Xie, Hui Xu, Congying Zhao, Yating Fang, Yongsong Zhou, Qiong Lan, Chunmei Shen, Bofeng Zhu   +7 more
doaj   +1 more source

Development of a multiplex event-specific PCR assay for detection of genetically modified rice [PDF]

, 2016
Global rice supplies have been found contaminated with unapproved varieties of genetically modified (GM) rice in recent years, which has led to product recalls in several of countries.
Ji, G.Z., Li, G., Lin, S.L., Lu, J., Wu, Y.F., Xiu, W.M., Yang, D.L., Yang, J., Zhao, J.N.   +8 more
core   +1 more source

Detection of alpha-thalassemias by multiplex polymerase chain reaction

Clinical Chemistry, 1994
Abstract Although alpha-thalassemia is the most common genetic abnormality in the world, there is currently no routine laboratory method to definitively identify individuals who are affected. We describe a rapid and simple method that utilizes deletion-sensitive primers to amplify normal DNA sequences.
L J, Bowie, P L, Reddy, M, Nagabhushan, P, Sevigny   +3 more
openaire   +2 more sources

Usefulness of Stool Multiplex Polymerase Chain Reaction Assays in Patients with Acute Diarrhea

The Korean Journal of Gastroenterology, 2022
Background/Aims: There is a recent increase in the use of stool multiplex PCR assay-based diagnostic tests in patients with acute diarrhea. We used multiplex PCR assays to analyze the distribution of diarrhea-causing bacteria and viruses, as well as the ...
Seo Hyun Kim, You Sun Kim, Seung Hyuk Kim, Won Eui Yoon, Hee Jun Myung, Jeong Seop Moon, Dong Hee Whang   +6 more
doaj   +1 more source

Occurrence of the Leucine-to-Phenylalanine knockdown Resistance (kdr) Mutation in Anopheles Arabiensis Populations in Tanzania, Detected by a Simplified High-Throughput SSOP-ELISA Method. [PDF]

, 2006
Molecular markers of insecticide resistance can provide sensitive indicators of resistance development in malaria vector populations. Monitoring of insecticide resistance in vector populations is an important component of current malaria control ...
Alifrangis, Michael, Drakeley, Chris, Kulkarni, Manisha A, Kweka, Eliningaya, Lyimo, Issa, Magesa, Stephen, Malima, Robert, Matowo, Johnson, Mosha, Frank W, Peter, Justin, Rau, Manfred E, Rowland, Mark, Salanti, Ali   +12 more
core   +3 more sources