Results 41 to 50 of about 129,024 (210)

Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR

open access: yesBioTechniques, 2009
Multiplex Manager 1.0 is a user-friendly cross-platform program that designs efficient combinations of existing genetic marker loci into multiplex polymerase chain reactions and optimizes using prior marker information. The program has the flexibility to
Clare E. Holleley, Paul G. Geerts
doaj   +1 more source

Streptococcus pneumoniaeType Determination by Multiplex Polymerase Chain Reaction

open access: yesJournal of Korean Medical Science, 2011
The purpose of this study was to develop pneumococcal typing by multiplex PCR and compare it with conventional serotyping by quellung reaction. Pneumococcal strains used in this study included 77 isolates from clinical specimens collected from children at Seoul National University Children's Hospital from 2006 to 2010.
Yun, Ki Wook   +4 more
openaire   +2 more sources

Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

open access: yesBiomédica: revista del Instituto Nacional de Salud, 2018
Introduction: Angiostrongyliasis is a disease caused by Angiostrongylus nematodes that is present worldwide. The infections with the highest impact on human and animal health are caused by A. cantonensis, A. costaricensis, and A. vasorum.
Rubén E. Varela-M   +2 more
doaj   +1 more source

Self-partitioning SlipChip for slip-induced droplet formation and human papillomavirus viral load quantification with digital LAMP [PDF]

open access: yes, 2020
Human papillomavirus (HPV) is one of the most common sexually transmitted infections worldwide, and persistent HPV infection can cause warts and even cancer.
Han, Xu   +8 more
core  

A novel cassette method for probe evaluation in the designed biochips [PDF]

open access: yes, 2014
A critical step in biochip design is the selection of probes with identical hybridisation characteristics. In this article we describe a novel method for evaluating DNA hybridisation probes, allowing the fine-tuning of biochips, that uses cassettes with ...
Al-humam, Abdulmohsen A.   +7 more
core   +4 more sources

Effectiveness of rapid multiplex polymerase chain reaction for early diagnosis and treatment of pertussis

open access: yesJournal of Microbiology, Immunology and Infection, 2021
Background: Pertussis, is an infectious respiratory disease caused by Bordetella pertussis. The incidence of pertussis has been increasing in South Korea to due to waning vaccine-induced immunity. Culture has a low sensitivity and a long turnaround time (
Se Chang Oh   +8 more
doaj   +1 more source

Multiplex STR amplification sensitivity in a silicon microchip [PDF]

open access: yes, 2018
The demand for solutions to perform forensic DNA profiling outside of centralized laboratories is increasing. We here demonstrate highly sensitive STR amplification using a silicon micro-PCR (mu PCR) chip.
Colle, Frederik   +8 more
core   +2 more sources

Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium [PDF]

open access: yes, 2015
© 2015 Webster et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
A. A. Hamidou   +23 more
core   +4 more sources

Multiplex polymerase chain reaction detection of enteropathogens in sewage in Norway

open access: yesActa Veterinaria Scandinavica, 2019
The primary objective of this small-scale study was to investigate the occurrence of enteropathogens in sewage (municipal wastewater) in Norway using the commercially available FilmArray® multiplex polymerase chain reaction (PCR) system with the ...
Øyvind Ørmen   +2 more
doaj   +1 more source

A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea

open access: yesBMC Infectious Diseases, 2021
Background Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work
Frieder Schaumburg   +2 more
doaj   +1 more source

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