Results 41 to 50 of about 465,638 (353)

Paper-Based Multiplex Surface-Enhanced Raman Scattering Detection Using Polymerase Chain Reaction Probe Codification.

Analytical Chemistry, 2021
We construct a multiplex surface-enhanced Raman scattering (SERS) platform based on a plasmonic paper substrate and a double-labeled probe for the detection of multiple fluorescent dyes at high sensitivity in a single-wavelength light source system ...
Eun Ju Kim, Hanbi Kim, Eunkyoung Park, Taekyung Kim, D. Chung, Young-Man Choi, Minhee Kang   +6 more
semanticscholar   +1 more source

Single-Tube Multiplex Polymerase Chain Reaction for the Detection of Genes Encoding Enterobacteriaceae Carbapenemase.

Japanese journal of infectious diseases (Print), 2019
A multiplex PCR assay in a single tube was developed for the detection of the carbapenemase genes of Enterobacteriaceae. Primers were designed to amplify the following six carbapenemase genes: blaKPC, blaIMP, blaNDM, blaVIM, blaOXA-48-like, and blaGES ...
M. Watahiki, R. Kawahara, Masahiro Suzuki, M. Aoki, K. Uchida, Y. Matsumoto, Y. Kumagai, Makiko Noda, Kanako Masuda, Chiemi Fukuda, Seiya Harada, Keiko Senba, Masato Suzuki, M. Matsui, Satowa Suzuki, K. Shibayama, H. Shinomiya   +16 more
semanticscholar   +1 more source

Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR

BioTechniques, 2009
Multiplex Manager 1.0 is a user-friendly cross-platform program that designs efficient combinations of existing genetic marker loci into multiplex polymerase chain reactions and optimizes using prior marker information. The program has the flexibility to
Clare E. Holleley, Paul G. Geerts
doaj   +1 more source

Effectiveness of rapid multiplex polymerase chain reaction for early diagnosis and treatment of pertussis

Journal of Microbiology, Immunology and Infection, 2021
Background: Pertussis, is an infectious respiratory disease caused by Bordetella pertussis. The incidence of pertussis has been increasing in South Korea to due to waning vaccine-induced immunity. Culture has a low sensitivity and a long turnaround time (
Se Chang Oh, Soo Min Park, Jian Hur, Eun Young Choi, Hyun Jung Jin, Yu Kyung Kim, Jong Ho Lee, Ji Young Ahn, Jae Min Lee   +8 more
doaj   +1 more source

Streptococcus pneumoniaeType Determination by Multiplex Polymerase Chain Reaction

Journal of Korean Medical Science, 2011
The purpose of this study was to develop pneumococcal typing by multiplex PCR and compare it with conventional serotyping by quellung reaction. Pneumococcal strains used in this study included 77 isolates from clinical specimens collected from children at Seoul National University Children's Hospital from 2006 to 2010.
Yun, Ki Wook, Cho, Eun Young, Hong, Ki Bae, Choi, Eun Hwa, Lee, Hoan Jong   +4 more
openaire   +2 more sources

Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

Biomédica: revista del Instituto Nacional de Salud, 2018
Introduction: Angiostrongyliasis is a disease caused by Angiostrongylus nematodes that is present worldwide. The infections with the highest impact on human and animal health are caused by A. cantonensis, A. costaricensis, and A. vasorum.
Rubén E. Varela-M, Jinney Stefany Arias, Luz Elena Velásquez   +2 more
doaj   +1 more source

Forensic validation studies of a novel 35-InDel multiplex polymerase chain reaction system

Journal of Forensic Science and Medicine, 2023
Background: A difficulty associated with forensic applications is the detection of degraded biological materials. Due to the large amplicon sizes of short tandem repeat alleles, valid genotyping results cannot be obtained from degraded biological ...
Tong Xie, Hui Xu, Congying Zhao, Yating Fang, Yongsong Zhou, Qiong Lan, Chunmei Shen, Bofeng Zhu   +7 more
doaj   +1 more source

A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea

BMC Infectious Diseases, 2021
Background Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work
Frieder Schaumburg, Neele Froböse, Robin Köck   +2 more
doaj   +1 more source

Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology

Molecular Oncology, EarlyView.
Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts, Senada Koljenović, Elise Daems, Karolien De Wael, Marc Peeters, Léon C. van Kempen, Greetje Vanhoutte, Karen Zwaenepoel, Timon Vandamme   +8 more
wiley   +1 more source

Multiplex polymerase chain reaction detection of enteropathogens in sewage in Norway

Acta Veterinaria Scandinavica, 2019
The primary objective of this small-scale study was to investigate the occurrence of enteropathogens in sewage (municipal wastewater) in Norway using the commercially available FilmArray® multiplex polymerase chain reaction (PCR) system with the ...
Øyvind Ørmen, Kristian Aalberg, Elisabeth Henie Madslien   +2 more
doaj   +1 more source