Results 1 to 10 of about 44,983 (186)

In vitro HIV DNA integration in STAT3 drives T cell persistence-A model of HIV-associated T cell lymphoma. [PDF]

PLoS Pathogens
Oncogenic retroviruses are known for their pathogenesis via insertional mutagenesis, in which the presence of a provirus and its transcriptional control elements alter the expression of a nearby or surrounding host gene.
Michael Rist, Machika Kaku, John M Coffin   +2 more
doaj   +2 more sources

Long-distance effects of insertional mutagenesis. [PDF]

PLoS ONE, 2011
Most common systems of genetic engineering of mammalian cells are associated with insertional mutagenesis of the modified cells. Insertional mutagenesis is also a popular approach to generate random alterations for gene discovery projects.
Ruchi Singhal, Xiaotao Deng, Alex A Chenchik, Eugene S Kandel   +3 more
doaj   +4 more sources

Insertional Mutagenesis of Chlamydomonas reinhardtii

Bio-Protocol, 2015
The unicellular microalga Chlamydomonas reinhardtii (C. reinhardtii) has been used as a reference model for numerous fields of research. Principle research areas are eukaryotic flagellar structure and function, basal bodies (centrioles), cell-cell ...
Chia-Hong Tsai, Christoph Benning
doaj   +2 more sources

Novel mRNA-Engineered Fully Human CAR-T Cells Targeting AXL in Solid Tumors [PDF]

Biomedicines
Background/Objectives: The AXL receptor tyrosine kinase is a promising therapeutic target in solid tumors, yet conventional viral vector-engineered CAR-T cells face critical limitations, including risks of insertional mutagenesis and immunogenicity from ...
Bo Zou, Mengge Wang, Shimeng Bai, Ning Li, Zhongyi Fan, Yuanzheng Peng, Mingshu Han, Chen Zeng, Hongzhou Lu, Lin Qi, Xingding Zhang, Xiaohua Tan, Qibin Liao   +12 more
doaj   +2 more sources

Insertional mutagenesis in zebrafish [PDF]

Developmental Dynamics, 2003
AbstractInsertional mutagenesis is a method for identifying genes essential for a given biological process by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene. The use of retrovirus‐mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes ...
Adam Amsterdam, Nancy Hopkins
openaire   +3 more sources

Deregulated Nras expression in knock-in animals harboring a gammaretroviral long terminal repeat at the Nras/Csde1 locus. [PDF]

PLoS ONE, 2013
To investigate mechanisms and phenotypic effects of insertional mutagenesis by gammaretroviruses, we have developed mouse lines containing a single Akv 1-99 long terminal repeat (LTR) and a floxed PGK/Tn5 neomycin cassette at the Nras proto-oncogene at ...
Borja Ballarín-González, Louise Berkhoudt Lassen, Randi Jessen, Annette Füchtbauer, Ernst-Martin Füchtbauer, Finn Skou Pedersen   +5 more
doaj   +1 more source

Insertional mutagenesis strategies in zebrafish [PDF]

Genome Biology, 2007
We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mechanisms and concepts of new transposon-based mutagenesis approaches under development, including prospects for conditional 'gene trapping' and ...
Sivasubbu, Sridhar, Balciunas, Darius, Amsterdam, Adam, Ekker, Stephen C   +3 more
openaire   +3 more sources

Transposon Insertional Mutagenesis in Rice, [PDF]

Plant Physiology, 2001
Transposon mutagenesis systems are being developed in rice to address gene functions using forward and reverse genetics strategies. Although the well-characterized maize ( Zea mays ) transposons seem an obvious choice to develop efficient transposon-tagging systems for gene knockouts and ...
Greco, R., Ouwerkerk, P.B.F., Sallaud, C., Kohli, A., Colombo, L., PuigdomOnech, P., Guiderdoni, E., Christou, P., Hoge, J.H.C., Pereira, A.   +9 more
openaire   +5 more sources

In vivo insertion pool sequencing identifies virulence factors in a complex fungal-host interaction. [PDF]

PLoS Biology, 2018
Large-scale insertional mutagenesis screens can be powerful genome-wide tools if they are streamlined with efficient downstream analysis, which is a serious bottleneck in complex biological systems.
Simon Uhse, Florian G Pflug, Alexandra Stirnberg, Klaus Ehrlinger, Arndt von Haeseler, Armin Djamei   +5 more
doaj   +1 more source

Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics

Plants, 2019
Insertional mutagenesis is an indispensable tool for engendering a mutant population using exogenous DNA as the mutagen. The advancement in the next-generation sequencing platform has allowed for faster screening and analysis of generated mutated ...
Hasthi Ram, Praveen Soni, Prafull Salvi, Nishu Gandass, Ankita Sharma, Amandeep Kaur, Tilak Raj Sharma   +6 more
doaj   +1 more source