General and Specific Promotion of Flagellar Assembly by a Flagellar Nucleoside Diphosphate Kinase [PDF]
, 2017 Nucleoside diphosphate kinases (NDKs) play a central role in diverse cellular processes using the canonical NDK activity or alternative mechanisms that remain poorly defined.
Ciruelas, Kristine S. +9 more
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The use of retroviral vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery [PDF]
, 2004 © 2004 Anson; licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction
Donald S Anson
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Nonmycorrhizal (Myc¯) Mutants of Hebeloma cylindrosporum Obtained Through Insertional Mutagenesis
Molecular Plant-Microbe Interactions, 2004 Polyethylene glycol-mediated transformation of protoplasts was used as a method for insertional mutagenesis to obtain mutants of the ectomycorrhizal fungus Hebeloma cylindrosporum impaired in symbiotic ability.
Jean-Philippe Combier +5 more
doaj +1 more source
Targeted insertional mutagenesis libraries for deep domain insertion profiling [PDF]
Nucleic Acids Research, 2019 AbstractDomain recombination is a key principle in protein evolution and protein engineering, but inserting a donor domain into every position of a target protein is not easily experimentally accessible. Most contemporary domain insertion profiling approaches rely on DNA transposons, which are constrained by sequence bias.
Willow Coyote-Maestas +4 more
openaire +4 more sources
Microbiology Spectrum, 2023 Transmission of the deadly malaria parasite Plasmodium falciparum from humans to mosquitoes is achieved by specialized intraerythrocytic sexual forms called gametocytes.
Jyotsna Chawla +13 more
doaj +1 more source
Medicago truncatula functional genomics: an invaluable resource for studies on agriculture sustainability [PDF]
, 2012 Legume functional genomics has moved many steps forward in the last two decades thanks to the improvement of genomics technologies and to the efforts of the research community.
Calderini, Ornella +2 more
core
Retroviral-mediated Insertional Mutagenesis in Zebrafish [PDF]
, 2011 Since the initial publication of this chapter in 2004, additional methodologies have been developed which could improve and/or complement the original retroviral-mediated insertional mutagenesis. Retroviral vectors have also been shown to be useful for goals other than mutagenesis.
Adam, Amsterdam +2 more
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Modeling integration site data for safety assessment with MELISSA
Nature CommunicationsGene and cell therapies pose safety concerns due to potential insertional mutagenesis by viral vectors. We introduce MELISSA, a regression-based statistical framework for analyzing Integration Site (IS) data to assess insertional mutagenesis risk, by ...
Tsai-Yu Lin +9 more
doaj +1 more source
Retroviral insertional mutagenesis identifies oncogene cooperation [PDF]
Cancer Science, 2005 Retroviral insertional mutagenesis has been applied to identify oncogenes that are important for both human and rodent carcinogenesis. The method reveals not only primary oncogenes but also cooperative genes that might be affected as second or third hits in multistep carcinogenesis.
openaire +2 more sources
A novel approach to identify driver genes involved in androgen-independent prostate cancer [PDF]
, 2014 Background Insertional mutagenesis screens have been used with great success to identify oncogenes and tumor suppressor genes. Typically, these screens use gammaretroviruses (γRV) or transposons as insertional mutagens.
Bii, Victor +6 more
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