Results 11 to 20 of about 209,529 (231)
BMC Biotechnology, 2008 Background Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Naismith James H, Liu Huanting
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BMC Biotechnology, 2004 Background Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by-passes any intermediate ...
Duggleby Ronald G +2 more
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Journal of Lipid Research, 1998 Both apolipoprotein (apo) E2 and apoE-Leiden (tandem repeat of amino acids 121–127) are associated with type III hyperlipoproteinemia and bind defectively to low density lipoprotein receptors. Removing the carboxyl terminus of both variants (residues 192–
Li-Ming Dong +4 more
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In silico Analysis and Site-directed Mutagenesis of Promoters
Bio-Protocol, 2017 In normal as in cancerous cells, gene expression is tightly regulated by transcription factors, which are responsible for up- or down-regulation of thousands of targets involved in different cell processes. Transcription factors can directly regulate the
Salah Boudjadi, Jean-François Beaulieu
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Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile [PDF]
Archives of Biological Sciences, 2012 A site-directed mutant R453T of a laccase from Thermus thermophilus HB27 (Tth-laccase) was constructed in order to investigate the effect on laccase catalytic properties. The mutated gene was cloned and overexpressed in Escherichia coli.
Liu Xin, Tian Meng, Liu Kewu
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Improved immobilization of laccase on a glassy carbon electrode by oriented covalent attachment [PDF]
Archives of Biological Sciences, 2014 A laccase from Thermus thermophilus HB27 was reported to be potentially useful in the design of a temperature controlled biofuel cell. For enhancing its application in different thermal conditions, we engineered a laccase-oriented immobilized ...
Liu Xin, Liu Kewu
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Current status and emerging frontiers in enzyme engineering: An industrial perspective
HeliyonProtein engineering mechanisms can be an efficient approach to enhance the biochemical properties of various biocatalysts. Immobilization of biocatalysts and the introduction of new-to-nature chemical reactivities are also possible through the same ...
Obinna Giles Ndochinwa +6 more
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Bio-Protocol, 2014 Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative ...
Tao Zhan
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Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10
FEBS Letters, EarlyView.Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang +8 more
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Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
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