Results 201 to 210 of about 347,517 (297)

Structural Insights Into CO2 Transport Pathways in a W‐Formate Dehydrogenase: Structural Basis for CO2 Reduction

Angewandte Chemie, EarlyView.
Integrated structural analysis of N. vulgaris formate dehydrogenase AB, an enzyme with applications in climate change mitigation, is reported. The substrate/product diffusion pathways were fully mapped, and a retention site was identified that transiently holds substrates and has a key role in CO2‐reducing activity.
Guilherme Vilela‐Alves, Rita Rebelo Manuel, Guilherme Martins, Philippe Carpentier, Agata Raczyńska, Maciej Szaleniec, Inês A. Cardoso Pereira, Maria João Romão, Cristiano Mota   +8 more
wiley   +2 more sources

Method of Site-Directed Mutagenesis Using Long Primer-Unique Site Elimination and Exonuclease III

BioTechniques, 1997
G. Nicolas, S. Pedroni, C. Fournier, H. Gautero, M.-C. Lecomte   +4 more
doaj   +1 more source

Tuning aromatic cage occupancy in prenyltransferases enables selective and efficient production of rare c-prenylated flavonoids. [PDF]

Nat Commun
Qiu R, Huang H, Chi J, Gao L, Gao Q, Li M, Cai M, Yu H, Wang S, Qian Z, Jiang M, Liu Y, Chen B, Zhou Y, Zhang ZM, Wang JB.   +15 more
europepmc   +1 more source

P450 engineering via structure-guided rational design achieves high C21-selectivity and bioconversion in steroid biosynthesis. [PDF]

Synth Syst Biotechnol
Yang J, Li R, Gao Q, Ma L, Wang Q, Ma G, Fan Y, Li S, Xu LH.   +8 more
europepmc   +1 more source

Comments on a Recently Published PCR Method for Site-Directed Mutagenesis

BioTechniques, 1996
Ghanshyam D. Heda
doaj   +1 more source

Use of gain-of-function suppressor screening to elucidate <i>Candida albicans</i> Cdr1 efflux mechanism, inhibition, and drug resistance. [PDF]

Front Microbiol
Zhao M, Niimi M, Cannon RD, Lamping E.
europepmc   +1 more source

Efficient Degradation of Monoacylglycerols by an Engineered <i>Aspergillus oryzae</i> Lipase: Synergistic Effects of sfGFP Fusion and Rational Design. [PDF]

Molecules
Wang Y, Liu F, Tian Y, Sun J, Liu D, Li F, Wang Y, Rao B.   +7 more
europepmc   +1 more source

Identification and characterization of GHR gene promoter of yak. [PDF]

Sci Rep
Wu X, Chu M, Shi Y, Wang F, Ma X, Ge Q, Guo X, Liang C, Yan P.   +8 more
europepmc   +1 more source

Lipid Pocket Binders Impose Allosteric Changes of Protein Dynamics Around the Active Site of the Protein Kinase p38α

Angewandte Chemie International Edition, EarlyView.
Used in search of novel allosteric sites in the serine/threonine kinase p38α, NMR spectroscopy reveals a dynamic coupling between the catalytic and lipid pockets. The findings uncover a motional interdependence that links distant regions of the enzyme, highlighting the lipid pocket as a promising site for allosteric intervention.
Sara Medina Gómez, Laurin T. Homberg, Mike Bührmann, Daniel Rauh, Rasmus Linser   +4 more
wiley   +1 more source

Green bioconversion of insoluble chitin: chitinase development pathways via multi-strategy synergy. [PDF]

Bioresour Bioprocess
Sai ZP, Yin YR, Yang LQ, Wang JH, Yang XY, Liu FX, Jing X, Zhang Y, Li YD, Sang P, Yang ZF.   +10 more
europepmc   +1 more source