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High-Throughput Site-Directed Mutagenesis
2019Protein engineering has an array of uses: whether you are studying a disease mutation, removing undesirable sequences, adding stabilizing mutations for structural purposes, or simply dissecting protein function. Protein engineering is almost exclusively performed using site-directed mutagenesis (SDM) as this provides targeted modification of specific ...
Claire, Strain-Damerell +1 more
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Trends in Neurosciences, 1984
Much of what neurons do they do with proteins. From functional studies we learn how these proteins operate, and from chemical and molecular biological research we learn about their molecular structures. But how are studies of structure and function to be connected?
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Much of what neurons do they do with proteins. From functional studies we learn how these proteins operate, and from chemical and molecular biological research we learn about their molecular structures. But how are studies of structure and function to be connected?
openaire +1 more source
[14] Site-directed mutagenesis
1995Publisher Summary This chapter discusses the design and implementation of site-directed mutagenesis approaches to elucidating the structure–function relationships of proteins. Before the development of recombinant deoxyribonucleic acid (DNA) technology, the structure–function relationships of proteins were explored by chemical modification of the ...
Tung Ming Fong +2 more
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DiRect: Site-directed mutagenesis method for protein engineering by rational design
Biochemical and Biophysical Research Communications, 2021Satoru S Watanabe, Takanori Kigawa
exaly