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Determinants of substrate specificity in mint pulegone reductase, a double bond reductase of the medium-chain dehydrogenase/reductase superfamily. [PDF]
J Biol ChemAndrews AJ +3 more
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Engineering of ω-transaminases for advanced applications. [PDF]
Appl Microbiol BiotechnolWegner U, von Wirén N, Kunze G.
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, 2010 The technique of site-directed mutagenesis has been used to characterize gene and protein structure-function relationships, protein-protein interactions, binding domains of proteins, or active sites of enzymes for the last three decades. In this technique, a nucleotide sequence of interest is experimentally altered using synthetic oligonucleotides. The
Carrigan, Patricia E. +2 more
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In Vitro Site Directed Mutagenesis
2023Site-Directed Mutagenesis (SDM) allows for changes in the DNA sequence of plasmids using polymerase chain reaction (PCR). It is a reliable, accessible, and rapid method which is the common initial step of many biochemial or genetic experiments. Here we describe the various different forms of SDM before giving a detailed method for the introduction of ...
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2013
Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational ...
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Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational ...
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Site-Directed Mutagenesis of Bifidobacterium Strains
2021At present, only a limited number of Bifidobacterium species are amenable to genetic manipulation using mutagenesis. This lack of genetic accessibility among the majority of bifidobacterial strains represents a significant roadblock for the study of gene function and expression in these potential probiotics.
Kieran, James, Douwe, van Sinderen
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Site-Directed Mutagenesis in the Mouse
1993Publisher Summary This chapter presents site-directed mutagenesis in the mouse. The correlation of genotype and phenotype is a central theme in the analysis of development. In the mouse, this type of reverse genetics is constrained not only by the size of the murine genome, which makes positional cloning difficult, but also by the limited ...
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Inverse PCR for Site-Directed Mutagenesis
2023Inverse PCR is a powerful tool for the rapid introduction of desired mutations at desired positions in a circular double-stranded DNA sequence. In this technique, custom-designed mutant primers oriented in the inverse direction are used to amplify the entire circular template with incorporation of the required mutation(s).
Diogo, Silva +4 more
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Trends in Plant Science, 2000
A system has been developed for site-directed mutagenesis in plants. It has been dubbed the pinpoint technique because it takes advantage of the plant’s DNA repair system and makes use of specifically designed hairpin-shaped molecules of DNA and RNA.
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A system has been developed for site-directed mutagenesis in plants. It has been dubbed the pinpoint technique because it takes advantage of the plant’s DNA repair system and makes use of specifically designed hairpin-shaped molecules of DNA and RNA.
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1983
In the preceding chapters dealing with modifications of nucleic acids it is assumed that, in general, these chemical reactions are randomly located along the genome. Exceptions occur when protein-nucleic interactions or nucleic acid secondary structure playa role.
B. Singer, D. Grunberger
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In the preceding chapters dealing with modifications of nucleic acids it is assumed that, in general, these chemical reactions are randomly located along the genome. Exceptions occur when protein-nucleic interactions or nucleic acid secondary structure playa role.
B. Singer, D. Grunberger
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