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Pyridoxal 5'-Phosphate-Dependent Enzymatic Decarboxylative Annulation. [PDF]

J Am Chem Soc
Chai W, Luo S, Xi W, He X, Zhang T, Zou Y, Hai Y.   +6 more
europepmc   +1 more source

Photoenzymatic Hydroalkylation Enables Streamlined Access to Aryl Glutarimide Precursors. [PDF]

Angew Chem Int Ed Engl
Xu Z, Mukherjee P, Gossert S, Thomas S, Vasilev VH, Welin ER, Tan Y, McKenna SM, Emmanuel MA, Hyster TK.   +9 more
europepmc   +1 more source

Clock-Turning Control of P450 TxtE Regioselectivity Enables Precise Site Functionalization of Aromatic Compounds. [PDF]

JACS Au
Yang X, Pan Y, Miao T, Yuan Z, Cong Y, Fang B, Yan X, Zhang J, Chen R, Feng Y, Zhang Z, Gao B, Zhang L.   +12 more
europepmc   +1 more source

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In Vitro Site Directed Mutagenesis

2023
Site-Directed Mutagenesis (SDM) allows for changes in the DNA sequence of plasmids using polymerase chain reaction (PCR). It is a reliable, accessible, and rapid method which is the common initial step of many biochemial or genetic experiments. Here we describe the various different forms of SDM before giving a detailed method for the introduction of ...
openaire   +2 more sources