Results 251 to 260 of about 3,838,500 (295)
Some of the next articles are maybe not open access.
Philosophical Transactions of the Royal Society of London. Series A, Mathematical and Physical Sciences, 1982
Abstract The development of genetic engineering, whereby a specific gene or cDNA (c is copy or complementary) can be isolated as part of a minichromosome that can replicate and be expressed (by transcription and translation) in a living cell, has made possible in vitro techniques for micromanipulation (i.e.
openaire +1 more source
Abstract The development of genetic engineering, whereby a specific gene or cDNA (c is copy or complementary) can be isolated as part of a minichromosome that can replicate and be expressed (by transcription and translation) in a living cell, has made possible in vitro techniques for micromanipulation (i.e.
openaire +1 more source
Activation of aspartase by site-directed mutagenesis
Biochemical and Biophysical Research Communications, 1991To elucidate the role of sulfhydryl groups in the enzymatic reaction of the aspartase from Escherichia coli, we used site-directed mutagenesis which showed that the enzyme was activated by replacement of Cys-430 with a tryptophan. This mutation produced functional alterations without appreciable structural change: The kcat values became 3-fold at pH 6 ...
S, Murase +5 more
openaire +2 more sources
Trends in Neurosciences, 1984
Much of what neurons do they do with proteins. From functional studies we learn how these proteins operate, and from chemical and molecular biological research we learn about their molecular structures. But how are studies of structure and function to be connected?
openaire +1 more source
Much of what neurons do they do with proteins. From functional studies we learn how these proteins operate, and from chemical and molecular biological research we learn about their molecular structures. But how are studies of structure and function to be connected?
openaire +1 more source
PCR-Mediated Site-Directed Mutagenesis
Cold Spring Harbor Protocols, 2013Unlike traditional site-directed mutagenesis, this protocol requires only a single PCR step using full plasmid amplification to generate point mutants. The method can introduce small mutations into promoter sites and is even better suited for introducing single or double mutations into proteins.
Michael F, Carey +2 more
openaire +2 more sources
High-Throughput Site-Directed Mutagenesis
2019Protein engineering has an array of uses: whether you are studying a disease mutation, removing undesirable sequences, adding stabilizing mutations for structural purposes, or simply dissecting protein function. Protein engineering is almost exclusively performed using site-directed mutagenesis (SDM) as this provides targeted modification of specific ...
Claire, Strain-Damerell +1 more
openaire +2 more sources