Results 51 to 60 of about 347,517 (297)
BioTechniques, 1999 Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins.
Alexander Turchin, Joseph F. Lawler
doaj +1 more source
Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma
Molecular Oncology, EarlyView.Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken +7 more
wiley +1 more source
Journal of Pharmacological Sciences, 2007 Based on radio-ligand binding and molecular modeling studies, sarpogrelate shows a moderate selectivity for 5-HT2B versus 5-HT2A receptors. To confirm the modeling data of sarpogrelate to 5-HT2B receptors predicting interaction of sarpogrelate towards ...
Habib Abul Muntasir +6 more
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Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve
Frontiers in Microbiology, 2021 Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt +14 more
doaj +1 more source
Site directed mutagenesis of L-pyrrolysine in dimethylamine methyltransferase [PDF]
, 2009 Methanosarcina barkeri is an archaeon and is able to use methylamines as growth substrates for methanogenesis. The methylamine methyltransferases that are involved in initiating methanogenesis from the methylamines have an in-frame amber codon (UAG) that
Patel, Jigar
core
Structural modeling and functional analysis of the essential ribosomal processing protease Prp from Staphylococcus aureus [PDF]
, 2017 In Firmicutes and related bacteria, ribosomal large subunit protein L27 is encoded with a conserved N-terminal extension that is removed to expose residues critical for ribosome function. Bacteria encoding L27 with this N-terminal extension also encode a
Christie, Gail E. +3 more
core +2 more sources
Hippo pathway at the crossroads of stemness and therapeutic resistance in breast cancer
Molecular Oncology, EarlyView.Dysregulation of the Hippo pathway drives nuclear accumulation of YAP/TAZ, activating stemness‐related transcriptional programs that sustain breast cancer stemness and fuel therapeutic resistance across subtypes, underscoring Hippo signaling as a targetable vulnerability. Figure created and edited with BioRender.com.
Giulia Schiavoni +11 more
wiley +1 more source
Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity
Biochemistry and Biophysics Reports, 2016 Human carboxylesterase 2 (hCES2) is a glycoprotein involved in the metabolism of drugs and several environmental xenobiotics, whose crystallization has been proved to be a challenging task.
Márcia Alves +7 more
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High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach
Bio-Protocol, 2020 Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is
Franziska Heydenreich +3 more
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Efficient method for site-directed mutagenesis in large plasmids without subcloning. [PDF]
PLoS ONE, 2017 Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids ...
Louay K Hallak +5 more
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