Results 11 to 20 of about 66,921 (262)

Electroporation of Mycobacteria [PDF]

Journal of Visualized Experiments, 2008
High efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly caused by the thick and waxy cell wall, but is compounded by the fact that most molecular techniques have been developed for distantly-related species such as Escherichia coli and Bacillus subtilis. In
Renan, Goude, Tanya, Parish
openaire   +3 more sources

Acylation of glycerolipids in mycobacteria

Nature Communications, 2023
We report on the existence of two phosphatidic acid biosynthetic pathways in mycobacteria, a classical one wherein the acylation of the sn-1 position of glycerol-3-phosphate (G3P) precedes that of sn-2 and another wherein acylations proceed in the ...
Shiva Kumar Angala, Ana Carreras-Gonzalez, Emilie Huc-Claustre, Itxaso Anso, Devinder Kaur, Victoria Jones, Zuzana Palčeková, Juan M. Belardinelli, Célia de Sousa-d’Auria, Libin Shi, Nawel Slama, Christine Houssin, Annaïk Quémard, Michael McNeil, Marcelo E. Guerin, Mary Jackson   +15 more
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Mycobacteria

Microbiology Spectrum, 2016
ABSTRACT The immunocompromised host is at increased risk of Mycobacterium tuberculosis complex and nontuberculous mycobacteria infection. Although Mycobacterium tuberculosis complex is a significant mycobacterial pathogen, nontuberculous mycobacteria causes substantial disease in ...
Patricia J, Simner, Gail L, Woods, Nancy L, Wengenack   +2 more
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Performance and impact of GeneXpert MTB/RIF® and Loopamp MTBC Detection Kit® assays on tuberculosis case detection in Madagascar

BMC Infectious Diseases, 2019
Background Tuberculosis rapid molecular assays, including GeneXpert MTB/RIF® and Loopamp MTBC Detection Kit®, are highly sensitive and specific. Such performance does not automatically translate in improved disease control and highly depends on their use,
Niaina Rakotosamimanana, Simon Grandjean Lapierre, Vaomalala Raharimanga, Mamy Serge Raherison, Astrid M. Knoblauch, Antso Hasina Raherinandrasana, Andrianantenaina Rakotoson, Julio Rakotonirina, Voahangy Rasolofo   +8 more
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Translational Rodent Models for Research on Parasitic Protozoa—A Review of Confounders and Possibilities

Frontiers in Cellular and Infection Microbiology, 2017
Rodents, in particular Mus musculus, have a long and invaluable history as models for human diseases in biomedical research, although their translational value has been challenged in a number of cases.
Totta Ehret, Totta Ehret, Francesca Torelli, Christian Klotz, Amy B. Pedersen, Frank Seeber   +5 more
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Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

BMC Biotechnology, 2020
Background Few bead-based multiplex assays have been described that detect antibodies against the protozoan parasite Toxoplasma gondii in large-scale seroepidemiological surveys. Moreover, each multiplex assay has specific variations or limitations, such
Sandra Klein, Daniel Stern, Frank Seeber
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Genome-Wide Association Studies (GWAS) Approaches for the Detection of Genetic Variants Associated with Antibiotic Resistance: A Systematic Review

Microorganisms, 2023
Antibiotic resistance is a significant threat to public health worldwide. Genome-wide association studies (GWAS) have emerged as a powerful tool to identify genetic variants associated with this antibiotic resistance.
Jeanneth Mosquera-Rendón, Claudia Ximena Moreno-Herrera, Jaime Robledo, Uriel Hurtado-Páez   +3 more
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Presence of multiple lesion types with vastly different microenvironments in C3HeB/FeJ mice following aerosol infection with Mycobacterium tuberculosis

Disease Models & Mechanisms, 2015
Cost-effective animal models that accurately reflect the pathological progression of pulmonary tuberculosis are needed to screen and evaluate novel tuberculosis drugs and drug regimens.
Scott M. Irwin, Emily Driver, Edward Lyon, Christopher Schrupp, Gavin Ryan, Mercedes Gonzalez-Juarrero, Randall J. Basaraba, Eric L. Nuermberger, Anne J. Lenaerts   +8 more
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The Orphan Response Regulator Rv3143 Modulates the Activity of the NADH Dehydrogenase Complex (Nuo) in Mycobacterium tuberculosis via Protein–Protein Interactions

Frontiers in Cellular and Infection Microbiology, 2022
Two-component signal transduction systems enable mycobacterial cells to quickly adapt and adequately respond to adverse environmental conditions encountered at various stages of host infection.
Renata Płocińska, Karolina Wasik, Przemysław Płociński, Przemysław Płociński, Ewelina Lechowicz, Magdalena Antczak, Ewelina Błaszczyk, Bożena Dziadek, Marcin Słomka, Anna Rumijowska-Galewicz, Jarosław Dziadek   +10 more
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From Entry to Early Dissemination—Toxoplasma gondii's Initial Encounter With Its Host

Frontiers in Cellular and Infection Microbiology, 2019
Toxoplasma gondii is a zoonotic intracellular parasite, able to infect any warm-blooded animal via ingestion of infective stages, either contained in tissue cysts or oocysts released into the environment.
Estefania Delgado Betancourt, Benjamin Hamid, Benedikt T. Fabian, Christian Klotz, Susanne Hartmann, Frank Seeber   +5 more
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