Results 91 to 100 of about 13,626,059 (252)

Conserved role of spike S2 domain N-glycosylation across betacoronaviruses

npj Viruses
Besides acting as an immunological shield, the N-glycans of SARS-CoV-2 are also critical for viral life cycle. As the S2 subunit of spike is highly conserved across betacoronaviruses, we determined the functional significance of the five ‘stem N-glycans’
Qi Yang, Anju Kelkar, Balaji Manicassamy, Sriram Neelamegham   +3 more
doaj   +1 more source

Immobilization of an Endo-β-N-acetylglucosaminidase for the Release of Bioactive N-glycans [PDF]

, 2018
As more is learned about glycoproteins' roles in human health and disease, the biological functionalities of N-linked glycans are becoming more relevant. Protein deglycosylation allows for the selective release of N-glycans and facilitates glycoproteomic
Daniela Barile, Barile, Daniela, de Moura Bell, Juliana MLN, Sercan Karav, Juliana M. L. N. De Moura Bell, Cohen, Joshua L, Cohen, Joshua L., Bell, Juliana M. L. N. de Moura, Joshua L. Cohen, Karav, Sercan   +9 more
core   +1 more source

Critical Roles of O‐Acetylation Revealed by Synthetic Trisaccharide Antigens for a Broad‐Spectrum Anti‐Salmonella Vaccine

Advanced Science, EarlyView.
A synthetic glycoconjugate vaccine based on a diacetylated Salmonella trisaccharide elicits broadly protective antibodies against four major human pathogenic serovars. Mechanistic studies identify 2,3‐di‐O‐acetylation of rhamnose as a critical determinant of antigen recognition and breadth of protection, establishing a new strategy for broad‐spectrum ...
Xingling Pan, Soham Maity, Herbert Kavunja, Yuvarani Murali, Ting‐An Chen, Chang‐xin Huo, Scott M. Baliban, Xuefei Huang   +7 more
wiley   +1 more source

A novel photocleavable amino-modified graphene for covalent purification of N-glycans from hepatocellular carcinoma patients’ serum for potential biomarkers discovery

Cancer Nanotechnology
Glycosylation alterations have emerged as significant indicators in the landscape of cancer diagnosis, with aberrant N-glycans standing out as potential biomarkers.
Xuyuan Chao, Shengjie Yang, Baoying Zhang, Xin Zang, Jingyi Zhang, Xizi Liu, Lu Chen, Lu Qi, Xiaofeng Xue, Han Hu, Xinghe Wang   +10 more
doaj   +1 more source

Detaching Photosensitive Nanoparticles from Cells Prevents Them from Remaining in Cells after Photoporation

Advanced Science, EarlyView.
Phenylboronic acid‐functionalized gold nanoparticles reversibly attach to cell‐surface glycans, enhancing photoporation‐mediated intracellular delivery. After irradiation, ATP competitively disrupts the boronic ester interactions and removes cell‐associated nanoparticles, markedly reducing residual gold.
Tao Lu, Mina Nikolić, Baihao Huang, Deep Punj, Herlinde De Keersmaecker, Thibaut Van Acker, Wenjia Xie, Florian Vanlauwe, Phillip Blondeel, Liesl De Graeve, Félix Sauvage, Ranhua Xiong, Chaobo Huang, Frank Vanhaecke, Kevin Braeckmans, Stefaan C. De Smedt   +15 more
wiley   +1 more source

In Planta Glycan Engineering and Functional Activities of IgE Antibodies

Frontiers in Bioengineering and Biotechnology, 2019
Human immunoglobulin E (IgE) is the most extensively glycosylated antibody isotype so glycans attached to the seven N-glycosites (NGS) in its Fab and Fc domains may modulate its functions.
Laura Montero-Morales, Daniel Maresch, Silvia Crescioli, Alexandra Castilho, Kristina M. Ilieva, Kristina M. Ilieva, Silvia Mele, Sophia N. Karagiannis, Sophia N. Karagiannis, Friedrich Altmann, Herta Steinkellner   +10 more
doaj   +1 more source

Immune Checkpoint Inhibitor as a Fifth Component of Lipid Nanoparticles Enables Lymph Node‐Targeting and Augments mRNA Vaccine Efficacy

Advanced Science, EarlyView.
Here, a PDL1 inhibitor is employed as a fifth component to modify lipid nanoparticles (LNPs). PDL1 inhibitor‐loaded LNPs show enhanced lymph node targeting, resulting in greater lymph node expansion, immune cell infiltration, and improved mRNA vaccine efficacy. This fifth component LNP could be a novel platform for mRNA vaccines.
Minki Ha, Jaehyun Kim, Seyoung Kim, Gonna Somu Naidu, Sungchan Cho, Seok‐Beom Yong   +5 more
wiley   +1 more source

Targeting branched N-glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade

Nature Communications
Aberrant glycosylation is a crucial strategy employed by cancer cells to evade cellular immunity. However, it’s unclear whether homologous recombination (HR) status-dependent glycosylation can be therapeutically explored.
Hao Nie, Pratima Saini, Taito Miyamoto, Liping Liao, Rafal J. Zielinski, Heng Liu, Wei Zhou, Chen Wang, Brennah Murphy, Martina Towers, Tyler Yang, Yuan Qi, Toshitha Kannan, Andrew Kossenkov, Hiroaki Tateno, Daniel T. Claiborne, Nan Zhang, Mohamed Abdel-Mohsen, Rugang Zhang   +18 more
doaj   +1 more source

High sensitivity profiling of N-glycans from mouse serum using fluorescent imidazolium tags by HILIC electrospray ionisation spectrometry [PDF]

N-linked glycosylation is a ubiquitous protein post-translational modification in which aberrant glycan biosynthesis has been linked to severe conditions like cancer.
Ghirardello, Mattia, Voglmeir, Josef, Liu, Li, Zhang, Si-Yu, Galan, M. Carmen [0000-0001-7307-2871], Galan, M Carmen, Hu, Zi-Xuan, Zhang, Yao-Yao, Ghirardello, Mattia [0000-0002-2855-4801]   +8 more
core   +1 more source

Determination of Gene‐Level Fitness Contributions Across the Mucin‐Utilization Network of Bacteroides thetaiotaomicron

Advanced Science, EarlyView.
Genome‐wide CRISPRi and RNA‐seq resolve how B. thetaiotaomicron converts host mucosal glycans into fitness. The study reveals a layered mucin‐utilization architecture in which capsular polysaccharides constrain surface access, extracellular CAZymes and peptidases show functional redundancy, and intracellular catabolic pathways create discrete metabolic
Kangsan Kim, Jiwon Lee, Minjeong Kang, Sung Sun Yim, Donghui Choe, Byung‐Kwan Cho   +5 more
wiley   +1 more source