Results 101 to 110 of about 10,628,077 (304)

Novel Endo-β-N-Acetylglucosaminidases Derived from Human Fecal Samples Selectively Release N-Glycans from Model Glycoproteins

Foods
Three novel endo-β-N-acetylglucosaminidases (AVUL01, BCAC01, and BFIN01) classified as members of the glucoside hydrolase (GH) family 18 were identified from human fecal samples and then cloned and characterized for their ability to hydrolyze two ...
Matthew Bolino, Nadini Haththotuwe Gamage, Hatice Duman, Odunayo Abiodun, Amilton S. De Mello, Sercan Karav, Steven A. Frese   +6 more
doaj   +1 more source

Nanomaterials for Allergy Diagnosis and Treatment: Advances, Opportunities and Translational Challenges

Advanced Science, EarlyView.
Nanomaterials offer dual applications in allergy management. For diagnosis, nanomaterials enhance analytical sensitivity and improve detection in specific IgE and functional assays such as the basophil activation test (BAT). For allergen‐specific immunotherapy, nanomaterials enable allergen masking and controlled release, and effectively modulate the ...
Madiha Habib, Shan Jiang, Joyce ZX Lee, CW Lim, HL Yeung, Nicki YH Leung, Patrick SC Leung, Ting F Leung, Christine YY Wai   +8 more
wiley   +1 more source

Immobilization of an Endo-β-N-acetylglucosaminidase for the Release of Bioactive N-glycans [PDF]

, 2018
As more is learned about glycoproteins' roles in human health and disease, the biological functionalities of N-linked glycans are becoming more relevant. Protein deglycosylation allows for the selective release of N-glycans and facilitates glycoproteomic
Daniela Barile, Barile, Daniela, de Moura Bell, Juliana MLN, Sercan Karav, Juliana M. L. N. De Moura Bell, Cohen, Joshua L, Cohen, Joshua L., Bell, Juliana M. L. N. de Moura, Joshua L. Cohen, Karav, Sercan   +9 more
core   +1 more source

A Cholesterol Analogue for Cell‐Surface Enzyme Display

Angewandte Chemie, EarlyView.
We report a non‐genetic strategy for bacterial cell surface enzyme immobilisation using cholesterol‐based artificial lipids containing Ni2+‐NTA binding groups that can integrate into biological membranes. The engineered cells can capture His‐tagged enzymes directly from cell lysates while preserving intracellular and surface activity, allowing for ...
Vasco F. Batista, Nele Van Wyngaerden, Changzhu Wu, Frank Glorius   +3 more
wiley   +2 more sources

A Chemically Defined Four‐Component Self‐Adjuvanting Tn Vaccine Activating Mincle, FcγR, and CD206 for Enhanced Antitumor Immunity

Advanced Science, EarlyView.
A fully synthetic four‐component vaccine has been developed combining Tn antigen, vizantin (Mincle agonist), rhamnose (antibody recruitment), and mannose (CD206 targeting). By synergistically activating Mincle, FcγR, and CD206 pathways, it elicits strong IgG switching and cytotoxic T‐cells, significantly inhibiting tumors in mice.
Wenbo Ming, Renyu Zhang, Xiaohui Li, Guiqi Li, Yu Niu, Deying Yang, Xiang Luo, Jun Liao, Zhongqiu Liu, Guochao Liao   +9 more
wiley   +1 more source

Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans. [PDF]

, 2016
Recently, the prevalence of Fasciola hepatica in some areas has increased considerably and the availability of a vaccine to protect livestock from infection would represent a major advance in tools available for controlling this disease.
Hokke, CH, Nguyen, DL, Sandra O’Neill (2637505), Cornelis H. Hokke (115502), Hokke, C.H., John P Dalton, O'Neill, Sandra, John P. Dalton (182938), Grace Mulcahy (2637496), Cwiklinski, K, Ravidà, Alessandra, Cwiklinski, K., Dalton, J.P., Andres Garcia-Campos, Alessandra Ravidà (151533), O'Neill, S., D Linh Nguyen, Hokke, Cornelis H, Garcia-Campos, A., Dalton, John P, Krystyna Cwiklinski (2637502), Cornelis H Hokke, Mulcahy, Grace, Grace Mulcahy, Andres Garcia-Campos (2637499), Garcia-Campos, Andres, Nguyen, D Linh, O’Neill, S, Nguyen, D.L., Cwiklinski, Krystyna, Dalton, JP, Mulcahy, G., D. Linh Nguyen (1916341), Krystyna Cwiklinski, Garcia-Campos, A, Sandra O'Neill, Mulcahy, G, Ravida, A., Alessandra Ravidà, Ravidà, A   +39 more
core   +1 more source

Total Chemical Synthesis of Interleukin‐15 and Interleukin‐2: Taming Protein Hydrophobicity and Aggregation by a Versatile Solubilizing Strategy

Angewandte Chemie, EarlyView.
A reducible solubilizing tag strategy (RST‐2.0) enables the synthesis, purification and ligation of difficult peptides while being readily removable just during folding, which overcomes the common challenges from protein hydrophobicity and aggregation. With this method, we achieved the first total synthesis of native and N‐glycosylated IL‐15 as well as
Jingwen Zeng, Haiyan Zhou, Wang Xia, Hongxiang Wu, Xuechen Li   +4 more
wiley   +2 more sources

Golgi self-correction generates bioequivalent glycans to preserve cellular homeostasis

eLife, 2016
Essential biological systems employ self-correcting mechanisms to maintain cellular homeostasis. Mammalian cell function is dynamically regulated by the interaction of cell surface galectins with branched N-glycans. Here we report that N-glycan branching
Haik Mkhikian, Christie-Lynn Mortales, Raymond W Zhou, Khachik Khachikyan, Gang Wu, Stuart M Haslam, Patil Kavarian, Anne Dell, Michael Demetriou   +8 more
doaj   +1 more source

Porcine kidney xenotransplantation: From primate models to clinical reality

Animal Models and Experimental Medicine, EarlyView.
In the face of a critical shortage of human donor kidneys for end‐stage renal disease patients, porcine kidney xenotransplantation has emerged as a viable solution. This field has navigated major hurdles, including immune rejection, physiological incompatibilities, potential biomechanical differences and the risk of cross‐species infection. To overcome
Zihang Guo, Ling Zhang, Shoulong Deng, Chuan Qin   +3 more
wiley   +1 more source

Fluorogenic Coupled Assays Reveal Catalytic Properties, Inhibition Constants and Cellular Location of Mucin‐Active Carbohydrate Sulfatases

Angewandte Chemie, EarlyView.
We report plate‐based coupled assays to rapidly and quantitatively determine the activity and selectivity of mucin‐active carbohydrate sulfatases. This fluorogenic assay further enables inhibitor identification and allows determination of (sub‐)cellular location. ABSTRACT Sulfated glycans play a central role in human health and influence cell signaling,
Charles W. E. Tomlinson, Madouc D. Bergers, David N. Bolam, Ana S. Luis, Alan Cartmell, Zachary Armstrong   +5 more
wiley   +2 more sources