Results 121 to 130 of about 10,628,077 (304)

Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

Journal of Analytical Methods in Chemistry, 2018
We developed a new method for MALDI-TOF MS detection of N-glycans derived from human serum. The synergistic combination of microwave-assisted Girard T derivatization, solid-phase extraction desalting, and an ionic liquid matrix (2, 5-dihydroxybenzoic ...
Hoa Thi Le, Kyu H. Park, Woong Jung, Hyung Soon Park, Tae Woo Kim   +4 more
doaj   +1 more source

In Planta Glycan Engineering and Functional Activities of IgE Antibodies

Frontiers in Bioengineering and Biotechnology, 2019
Human immunoglobulin E (IgE) is the most extensively glycosylated antibody isotype so glycans attached to the seven N-glycosites (NGS) in its Fab and Fc domains may modulate its functions.
Laura Montero-Morales, Daniel Maresch, Silvia Crescioli, Alexandra Castilho, Kristina M. Ilieva, Kristina M. Ilieva, Silvia Mele, Sophia N. Karagiannis, Sophia N. Karagiannis, Friedrich Altmann, Herta Steinkellner   +10 more
doaj   +1 more source

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

Hepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann, Marti Cabanes‐Creus, Moritz Ertelt, Renina Gale Navarro, Julie Lucifora, Qinggong Yuan, Karin Nien‐Huber, Ahmed Abdelrahman, Xuan‐Khang Vu, Liang Zhang, Ann‐Christin Franke, Christian Schmithals, Albrecht Piiper, Annabelle Vogt, Maria Gonzalez‐Carmona, Jochen T. Frueh, Evelyn Ullrich, Philip Meuleman, Steven R. Talbot, Margarete Odenthal, Michael Ott, Erhard Seifried, Clara T. Schoeder, Joachim Schwäble, Leszek Lisowski, Hildegard Büning   +25 more
wiley   +1 more source

A novel photocleavable amino-modified graphene for covalent purification of N-glycans from hepatocellular carcinoma patients’ serum for potential biomarkers discovery

Cancer Nanotechnology
Glycosylation alterations have emerged as significant indicators in the landscape of cancer diagnosis, with aberrant N-glycans standing out as potential biomarkers.
Xuyuan Chao, Shengjie Yang, Baoying Zhang, Xin Zang, Jingyi Zhang, Xizi Liu, Lu Chen, Lu Qi, Xiaofeng Xue, Han Hu, Xinghe Wang   +10 more
doaj   +1 more source

Identification of novel neutralizing determinants for protection against HCV

Hepatology, EarlyView., 2022
Identification of novel neutralizing determinants for protection against hepatitis C virus. Abstract Background and Aims HCV evasion of neutralizing antibodies (nAb) results in viral persistence and poses challenges to the development of an urgently needed vaccine.
Garazi P. Alzua, Anne F. Pihl, Anna Offersgaard, Rodrigo Velázquez‐Moctezuma, Carlos R. Duarte Hernandez, Elias H. Augestad, Ulrik Fahnøe, Christian K. Mathiesen, Henrik Krarup, Mansun Law, Jannick Prentoe, Jens Bukh, Judith M. Gottwein   +12 more
wiley   +1 more source

Ion mobility mass spectrometry for extracting spectra of N-glycans directly from incubation mixtures following glycan release : application to glycans from engineered glycoforms of intact, folded HIV gp120 [PDF]

, 2011
The analysis of glycosylation from native biological sources is often frustrated by the low abundances of available material. Here, ion mobility combined with electrospray ionization mass spectrometry have been used to extract the spectra of N-glycans ...
Christopher N. Scanlan, Crispin, Max, Scanlan, C.N., Scrivens, James H., Konstantinos Thalassinos, Camille Bonomelli, Snezana Vasiljevic, David J. Harvey, Scrivens, James, Sobott, F., Bonomelli, C., Frank Sobott, Charlotte A. Scarff, Vasiljevic, Snezana, James H. Scrivens, Crispin, M., Wrobel, Antoni, Vasiljevic, S., Wrobel, A., Scrivens, J.H., Crispin, Matthew, Scarff, Charlotte A., Scarff, C.A., Harvey, D.J., Scanlan, Christopher N., Thalassinos, Konstantinos, Bonomelli, Camille, Sobott, Frank, Harvey, David J., Max Crispin, Antoni Wrobel   +30 more
core   +1 more source

Targeting branched N-glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade

Nature Communications
Aberrant glycosylation is a crucial strategy employed by cancer cells to evade cellular immunity. However, it’s unclear whether homologous recombination (HR) status-dependent glycosylation can be therapeutically explored.
Hao Nie, Pratima Saini, Taito Miyamoto, Liping Liao, Rafal J. Zielinski, Heng Liu, Wei Zhou, Chen Wang, Brennah Murphy, Martina Towers, Tyler Yang, Yuan Qi, Toshitha Kannan, Andrew Kossenkov, Hiroaki Tateno, Daniel T. Claiborne, Nan Zhang, Mohamed Abdel-Mohsen, Rugang Zhang   +18 more
doaj   +1 more source

Profiling of N-linked Glycans from Human Serum with Oxidative Ordered Mesoporous Carbon

, 2009
Glycosylation is one of the most common forms of post-translational modifications on proteins, and glycoproteins account more than 50% in eukaryotic proteins.
秦洪强, 吴仁安, 邹汉法, 潘陈松, 陈锐, 赵梁   +5 more
core  

Biosynthesis of human-type N-glycans in heterollogous systems

, 2008
[[abstract]]Insects, yeasts and plants generate widely different N-glycans, the structures of which differ significantly from those produced by mammals.
Betenbaugh, MJ; Tomiya, N; Narang, S; Hsu, JTA; Lee, YC
core   +1 more source

RESOLVING EXTENDED N‐GLYCANS BY NMR: NEW INSIGHTS INTO INFLUENZA HEMAGGLUTININ N‐GLYCAN INTERACTIONS

The FASEB Journal, 2018
Seasonal flu (influenza virus infection) is one of the major threats to human health, causing of up to 500 thousands deaths worldwide annually.1,2 Hemagglutinin, a glycoprotein presented in the surface of the influenza virus, binds sialylated N‐glycans located on the surface of host epithelial cells. The structural elucidation and the
Beatriz Fernández de Toro Ronda, Wenjie Peng, Andrew Thompson, Gema Domínguez, F. Javier Cañada, Javier Pérez Castells, Jesús Jiménez Barbero, James C. Paulson, Ángeles Canales   +8 more
openaire   +1 more source