Results 21 to 30 of about 18,503 (241)

Tumour inhibitory activity on pancreatic cancer by bispecific nanobody targeting PD-L1 and CXCR4

BMC Cancer, 2022
Background: Antibodies and derivative drugs targeting immune checkpoints have been approved for the treatment of several malignancies, but there are fewer responses in patients with pancreatic cancer.
Shuai Hao, Shuyi Xu, Liangzhu Li, Yaxian Li, Meiqi Zhao, Junsheng Chen, Shunying Zhu, Yueqing Xie, Hua Jiang, Jianwei Zhu, Mingyuan Wu   +10 more
doaj   +1 more source

Nanobody-Based Lateral Flow Immunoassay for the Rapid Detection of Aflatoxin B1 in Almond Milk

, 2022
Aflatoxin B1 (AFB1) is the most potent genotoxic and carcinogenic aflatoxin produced by Aspergillus flavus and Aspergillus parasiticus. AFB1 is considered a major concern in food safety because of its high toxicity and possible contamination of a variety
Irene Gonzalez-Garcia (13174714), Bruce D. Hammock (65754), Maojun Jin (767084), Natalia Vasylieva (1548790), Justin B. Siegel (1333323), J.-Pablo Salvador (2020612), Ryan Caster (13174717)   +6 more
core   +1 more source

Nanobody-thioesterase chimeras to specifically target protein palmitoylation

 The complexity of the cellular proteome is massively expanded by a repertoire of chemically distinct reversible post-translational modifications (PTMs) that control protein localisation, interactions, and function.
William Fuller (3875263), Emma Dunning (20691914), William Mullen (103621), Caglar Gok (18964531), Fiona Jordan (5747414), Emily K. Gallen (20691911), Sheon Mary (3696952), Samuel Adu (20691908), Alan D. Robertson (19336523), Chien-Wen Kuo (13908834), Hannah Fulton (20697910), Godfrey L. Smith (8152389), Eleanor Dickson-Murray (20691905)   +12 more
core   +4 more sources

Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Microbial Cell Factories, 2023
Background Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single variable domains, and they have been
Manu De Groeve, Bram Laukens, Peter Schotte   +2 more
doaj   +1 more source

Rapid Delivery of Nanobodies/VHHs into Living Cells via Expressing In Vitro-Transcribed mRNA

Molecular Therapy: Methods & Clinical Development, 2020
Intracellular antigen labeling and manipulation by antibodies have been long-thought goals in the field of cell research and therapy. However, a central limitation for this application is that antibodies are not able to penetrate into the cytosol of ...
Xuechen Zhou, Rui Hao, Chen Chen, Zhipeng Su, Linhong Zhao, Zhuojuan Luo, Wei Xie   +6 more
doaj   +1 more source

sog-Nanobody;;GFP_embryo

, 2022
sog-Nanobody/+;;GFP/+ embryo with maternally loaded GFP, live imaged on a confocal microscope. Movies here show Z stacks at selected time points, and show a maximum intensity projection of the full time ...
Division of Molecular & Cellular Function, Frampton, Sophie   +1 more
core   +2 more sources

Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein–Protein Interaction Analysis, and Protein Function Exploration

Frontiers in Immunology, 2017
Over the last two decades, nanobodies or single-domain antibodies have found their way in research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid heavy chain only antibodies, possess remarkable characteristics that favor
Els Beghein, Jan Gettemans
doaj   +1 more source

Representative BLItz KD quantification of nanobody 26.

, 2023
(A) Sequential loading of five steps for Biolayer Interferometry Analysis of nanobody 26 (626.57 nM) at six concentrations of PRL-3. Baseline– 30 seconds of BLI buffer to equilibrate the biosensor; Loading– 30 seconds of nanobody incubation with ...
K. Martin Chow (772610), Louis B. Hersh (15852915), Zachary A. Williamson (15852912), Konstantin V. Korotkov (9090), Caroline N. Smith (15852909), Kyle Kihn (11876012), Daniel Deredge (6308525), Jessica S. Blackburn (14256575)   +7 more
core   +1 more source

Intratracheal trimerized nanobody cocktail administration suppresses weight loss and prolongs survival of SARS-CoV-2 infected mice

Communications Medicine, 2022
Nagata, Utsumi, Asaka, Maeda et al. describe a nanobody, TP86, that potently neutralizes both BA.1 and BA.2 Omicron SARS-CoV-2 variants, and, when combined with the TP17 nanobody, broadly neutralizes all SARS-CoV-2 variants.
Kayoko Nagata, Daichi Utsumi, Masamitsu N. Asaka, Ryota Maeda, Kotaro Shirakawa, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Yohei Yanagida, Yugo Kawai, Kei Sato, Yutaro Yamaoka, Kei Miyakawa, Akihide Ryo, Yasuhiro Yasutomi, Akihiro Imura, Akifumi Takaori-Kondo   +16 more
doaj   +1 more source

Preparation and characterization of a novel nanobody against T-cell immunoglobulin and mucin-3 (TIM-3) [PDF]

Iranian Journal of Basic Medical Sciences, 2016
Objective(s): As T-cell immunoglobulin and mucin domain 3 (TIM-3) is an immune regulatory molecule; its blocking or stimulating could alter the pattern of immune response towards a desired condition.
Vida Homayouni, Mazdak Ganjalikhani-hakemi, Abbas Rezaei, Hossein Khanahmad, Mahdi Behdani, Fatemeh Kazemi Lomedasht   +5 more
doaj   +1 more source