Results 31 to 40 of about 18,503 (241)
Nanobody-Mediated Neutralization Reveals an Achilles Heel for Norovirus
, 2020 Human norovirus frequently causes outbreaks of acute gastroenteritis. Although discovered more than five decades ago, antiviral development has, until recently, been hampered by the lack of a reliable human norovirus cell culture system.
Hansman, GS +5 more
core +1 more source
Diversity in kinetics correlated with structure in nano body-stabilized LacY.
PLoS ONE, 2020 The structure of lactose permease, stabilized in a periplasmic open conformation by two Gly to Trp replacements (LacYww) and complexed with a nanobody directed against this conformation, provides the highest resolution structure of the symporter.
Hemant Kumar +7 more
doaj +1 more source
Scientific Reports, 2021 A fusion protein comprising an antibody and a cationic peptide, such as arginine-9 (R9), is a candidate molecule for efficient and cell-specific delivery of siRNA into cells in order to reduce the side effects of nucleic acid drugs.
Yu Ando +4 more
doaj +1 more source
Nanobody binding can induce DFC localization.
, 2023 (A) Representative nucleoli from oocytes expressing Gar1 and Gar1M1 nucleolar localization and the same proteins fused with a GFP nanobody (Nb). These proteins were all fused with a red fluorescent protein and were co-expressed with Fbl-eGFP, a DFC ...
Maunika Gandhamaneni (17364772) +2 more
core +1 more source
Molecular Oncology, 2022 Alteration in glycosylation pattern of MUC1 mucin tandem repeats during carcinomas has been shown to negatively affect adhesive properties of malignant cells and enhance tumor invasiveness and metastasis.
Parnaz Merikhian +12 more
doaj +1 more source
Biotechnology & Biotechnological Equipment, 2021 Nanobodies belong to a new generation of antibodies produced by recombinant engineering and have high perspectives in biopharmaceutical drug formulations.
Mohamed Karam Qassas +3 more
doaj +1 more source
Nanobody-Targeted Photodynamic Therapy Selectively Kills Viral GPCR-Expressing Glioblastoma Cells
, 2019 Photodynamic therapy (PDT) eradicates tumors by the local activation of a photosensitizer with near-infrared light. One of the aspects hampering the clinical use of PDT is the poor selectivity of the photosensitizer.
Sabrina Oliveira (21108) +8 more
core +1 more source
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source