Results 41 to 50 of about 19,829 (313)

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction

Nature Communications, 2020
SARS-CoV-2 enters host cells through an interaction between the spike glycoprotein and the angiotensin converting enzyme 2 (ACE2) receptor. Directly preventing this interaction presents an attractive possibility for suppressing SARS-CoV-2 replication ...
L. Hanke, L. Perez, D. Sheward, H. Das, T. Schulte, Ainhoa Moliner Morro, M. Corcoran, A. Achour, G. K. Hedestam, B. Martin Hällberg, B. Murrell, G. McInerney   +11 more
semanticscholar   +1 more source

Nanobodies as molecular imaging probes

Free Radical Biology and Medicine, 2022
Camelidae derived single-domain antibodies (sdAbs), commonly known as nanobodies (Nbs), are the smallest antibody fragments with full antigen-binding capacity. Owing to their desirable properties such as small size, high specificity, strong affinity, excellent stability, and modularity, nanobodies are on their way to overtake conventional antibodies in
Sarah Barakat, Melike Berksöz, Pegah Zahedimaram, Sofia Piepoli, Batu Erman   +4 more
openaire   +4 more sources

NANOBODY® Molecule, a Giga Medical Tool in Nanodimensions

International Journal of Molecular Sciences, 2023
Although antibodies remain the most widely used tool for biomedical research, antibody technology is not flawless. Innovative alternatives, such as Nanobody® molecules, were developed to address the shortcomings of conventional antibodies.
Sarah Kunz, Manon Durandy, L. Seguin, C. Féral   +3 more
semanticscholar   +1 more source

Intratracheal trimerized nanobody cocktail administration suppresses weight loss and prolongs survival of SARS-CoV-2 infected mice

Communications Medicine, 2022
Nagata, Utsumi, Asaka, Maeda et al. describe a nanobody, TP86, that potently neutralizes both BA.1 and BA.2 Omicron SARS-CoV-2 variants, and, when combined with the TP17 nanobody, broadly neutralizes all SARS-CoV-2 variants.
Kayoko Nagata, Daichi Utsumi, Masamitsu N. Asaka, Ryota Maeda, Kotaro Shirakawa, Yasuhiro Kazuma, Ryosuke Nomura, Yoshihito Horisawa, Yohei Yanagida, Yugo Kawai, Kei Sato, Yutaro Yamaoka, Kei Miyakawa, Akihide Ryo, Yasuhiro Yasutomi, Akihiro Imura, Akifumi Takaori-Kondo   +16 more
doaj   +1 more source

An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

Science, 2020
Nanobodies that neutralize Monoclonal antibodies that bind to the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) show therapeutic promise but must be produced in mammalian cells and need to be delivered intravenously.
M. Schoof, B. Faust, R. Saunders, S. Sangwan, V. Rezelj, N. Hoppe, Morgane Boone, Christian B. Billesbølle, C. Puchades, C. Azumaya, H. Kratochvil, M. Zimanyi, I. Deshpande, Jiahao Liang, S. Dickinson, Henry C. Nguyen, C. Chio, G. Merz, M. Thompson, D. Diwanji, K. Schaefer, A. Anand, N. Dobzinski, B. Zha, C. Simoneau, K. Leon, K. White, U. S. Chio, Meghna Gupta, M. Jin, Fei Li, Yanxin Liu, Kaihua Zhang, D. Bulkley, Ming Sun, A. Smith, A. Rizo, F. Moss, A. Brilot, S. Pourmal, R. Trenker, T. Pospiech, Sayan Gupta, B. Barsi-Rhyne, V. Belyy, A. W. Barile-Hill, S. Nock, Yuwei Liu, N. Krogan, C. Ralston, D. Swaney, A. García-Sastre, M. Ott, M. Vignuzzi, P. Walter, A. Manglik   +55 more
semanticscholar   +1 more source

Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag

bioRxiv, 2022
Probing epigenomic marks such as histone modifications at a single cell level in thousands of cells has been recently enabled by technologies such as scCUT&Tag.
Marek Bartošovič, G. Castelo-Branco
semanticscholar   +1 more source

A bispecific monomeric nanobody induces spike trimer dimers and neutralizes SARS-CoV-2 in vivo

Nature Communications, 2022
Antibodies binding to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike have therapeutic promise, but emerging variants show the potential for virus escape.
L. Hanke, H. Das, D. Sheward, L. P. Vidakovics, E. Urgard, Ainhoa Moliner-Morro, Changil Kim, V. Karl, Alec Pankow, Natalie L. Smith, Bartłomiej Porębski, O. Fernández-Capetillo, E. Sezgin, G. Pedersen, J. Coquet, B. Martin Hällberg, B. Murrell, G. McInerney   +17 more
semanticscholar   +1 more source

Biofunctional Nanodot Arrays in Living Cells Uncover Synergistic Co‐Condensation of Wnt Signalodroplets

Small, Volume 18, Issue 50, December 15, 2022., 2022
High‐density arrays of biofunctionalized nanodots with diameters of 300 nm enable spatially controlled assembly of active Wnt signaling platforms in the plasma membrane. Massive parallelization at the single cell level uncovers receptor density‐dependent co‐condensation of cytosolic effector proteins, suggesting digitalized transmembrane signaling ...
Michael Philippi, Christian P. Richter, Marie Kappen, Isabelle Watrinet, Yi Miao, Mercedes Runge, Lara Jorde, Sergej Korneev, Michael Holtmannspötter, Rainer Kurre, Joost C. M. Holthuis, K. Christopher Garcia, Andreas Plückthun, Martin Steinhart, Jacob Piehler, Changjiang You   +15 more
wiley   +1 more source

Nanobody Technology: A Versatile Toolkit for Microscopic Imaging, Protein–Protein Interaction Analysis, and Protein Function Exploration

Frontiers in Immunology, 2017
Over the last two decades, nanobodies or single-domain antibodies have found their way in research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid heavy chain only antibodies, possess remarkable characteristics that favor
Els Beghein, Jan Gettemans
doaj   +1 more source

Nanobody interaction unveils structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant [PDF]

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. Volume 1865, Issue 3, 1 March 2019, Pages 648-660, 2019
AGel amyloidosis, formerly known as familial amyloidosis of the Finnish-type, is caused by pathological aggregation of proteolytic fragments of plasma gelsolin. So far, four mutations in the gelsolin gene have been reported as responsible for the disease.
arxiv   +1 more source