Effects of the tat and nef gene products of human immunodeficiency virus type 1 (HIV-1) on transcription controlled by the HIV-1 long terminal repeat and on cell growth in macrophages [PDF]
Journal of Virology, 1993 The RAW264 murine macrophage cell line was used as a model to examine the role of the tat and nef gene products in the transcription regulation of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in macrophages. Contrary to claims that the activity of the HIV-1 LTR responds poorly in rodent cells to trans activation by the ...
Murphy, Kathleen M. +3 more
core +9 more sources
Genetic characterization of human immunodeficiency virus type 1 nef gene products translated in vitro and expressed in mammalian cells [PDF]
Journal of Virology, 1991 Expression of the human immunodeficiency virus type 1 nef gene was studied by in vitro transcription-translation and by transfection into monkey COS cells. Two Nef-related peptides, of 27 and 25 kDa, were identified by immunoprecipitation with anti-Nef antibodies.
J, Kaminchik +5 more
openaire +3 more sources
A recombinant herpesviral vector containing a near-full-length SIVmac239 genome produces SIV particles and elicits immune responses to all nine SIV gene products. [PDF]
PLoS Pathogens, 2018 The properties of the human immunodeficiency virus (HIV) pose serious difficulties for the development of an effective prophylactic vaccine. Here we describe the construction and characterization of recombinant (r), replication-competent forms of rhesus ...
Young C Shin +8 more
doaj +2 more sources
Construction and characterization of highly infectious full-length molecular clones of a HIV-1 CRF07_BC isolate from Xinjiang, China. [PDF]
PLoS ONE, 2013 Among the various subtypes of the M group of human immunodeficiency virus type 1 (HIV-1), clade CRF07_BC is the most prevalent in China. To date, no strong replicable CRF07_BC infectious clone has been constructed.
Zheng Wang +7 more
doaj +2 more sources
The human immunodeficiency virus-1 nef gene product: a positive factor for viral infection and replication in primary lymphocytes and macrophages. [PDF]
The Journal of experimental medicine, 1994 Considerable controversy and uncertainty have surrounded the biological function of the Human Immunodeficiency Virus (HIV)-1 nef gene product. Initial studies suggested that this early, nonstructural viral protein functioned as a negative regulatory factor; thus, it was proposed to play a role in establishing or maintaining viral latency.
Miller, MD +4 more
openaire +5 more sources
Biosynthesis of rumbrins and inspiration for discovery of HIV inhibitors
Acta Pharmaceutica Sinica B, 2022 Investigation on how nature produces natural compounds with chemical and biological diversity at the genetic level offers inspiration for the discovery of new natural products and even their biological targets.
Beifen Zhong +7 more
doaj +1 more source
Formation of a Unique Cluster of G-Quadruplex Structures in the HIV-1 nef Coding Region: Implications for Antiviral Activity [PDF]
, 2013 G-quadruplexes are tetraplex structures of nucleic acids that can form in G-rich sequences. Their presence and functional role have been established in telomeres, oncogene promoters and coding regions of the human chromosome.
Frasson, Ilaria +8 more
core +10 more sources
Expression and biochemical characterization of human immunodeficiency virus type 1 nef gene product [PDF]
Journal of Virology, 1990 nef genes from human immunodeficiency virus type 1 isolates BH10 and LAV1 (lymphadenopathy-associated virus type 1) were expressed in Escherichia coli under the deo operon promoter. The two proteins found in the soluble compartment of the bacterial lysate were purified by ion-exchange column chromatography to apparent homogeneity.
J, Kaminchik +9 more
openaire +2 more sources
Expression, purification and biochemical characterisation of the human immunodificiency virus 1 nef gene product [PDF]
European Journal of Biochemistry, 1992 The human immunodeficiency virus 1 (HIV‐1) nef gene encoded by the HIV‐1 isolate lymphadenopathy‐associated virus type 1 was expressed in Escherichia coli under the control of the tac promoter. The protein is found mainly in the soluble part of the bacterial lysate; a simple twocolumn purification scheme has been developed allowing isolation of the ...
Wolber, V. +6 more
openaire +4 more sources
Assisted evolution enables HIV-1 to overcome a high trim5α-imposed genetic barrier to rhesus macaque tropism [PDF]
, 2013 Diversification of antiretroviral factors during host evolution has erected formidable barriers to cross-species retrovirus transmission. This phenomenon likely protects humans from infection by many modern retroviruses, but it has also impaired the ...
A Kuroishi +75 more
core +7 more sources