Results 111 to 120 of about 986,696 (337)

Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR Pesquisa de Neospora caninum em fetos bovinos por histologia, imunoistoquímica e nested-PCR

Revista Brasileira de Parasitologia Veterinária, 2009
Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB).
Aline Diniz Cabral, Clarice Neves Camargo, Nara Thiers Cacciatori Galleti, Liria Hiromi Okuda, Edviges Maristela Pituco, Claudia Del Fava   +5 more
doaj   +1 more source

Comparison of PCR, nested PCR, and random amplified\ud polymorphic DNA PCR for detection and typing\ud of Ureaplasma urealyticum in specimens\ud from pregnant women

, 1998
A PCR assay, using three primer pairs, was developed for the detection of Ureaplasma urealyticum, parvo\ud biovar, mba types 1, 3, and 6, in cultured clinical specimens.
Timms, Peter, Knox, Christine L.
core  

TNAP and PHOSPHO1 Function Synergistically to Afford Critical Control Over the Mineralization of the Postnatal Murine Skeleton

Advanced Science, EarlyView.
Biomineralization underpins skeletal development, yet its molecular control remains incompletely understood. Using a novel murine knockout model, this study reveals the essential and complementary roles of PHOSPHO1 and TNAP in postnatal skeletal development.
Lucie E. Bourne, Aikta Sharma, Scott Dillon, Jacob Keen, Soher N. Jayash, Natalie Crump, Lucinda A.E. Evans, Maya Karmali, Worachet Promruk, Claire E. Clarkin, Sonoko Narisawa, Louise Stephen, Brian L. Foster, José Luis Millán, Colin Farquharson, Katherine A. Staines   +15 more
wiley   +1 more source

Deteksi Mycobacterium tuberculosis dan resistensinya terhadap rifampisin dengan Metode nested polymerase chain reaction (PCR) dan sequencing

Universa Medicina, 2007
Latar Belakang DNA rpob (RNA polymerase sub unit b) Mycobacterium tuberculosis dapat diamplifikasi secara spesifik dengan metode nested polymerase chain reaction (PCR).
Maria Lina Rosilawati
doaj   +1 more source

SOX30 Facilitates Triple‐Negative Breast Cancer Metastasis via TNFR2–NF‐κB Signaling and Tumor Microenvironment Remodeling

Advanced Science, EarlyView.
SOX30 drives triple‐negative breast cancer (TNBC) metastasis through a dual mechanism: direct activation of the TNFR2/NF‐κB signaling cascade and subsequent CCL20‐mediated recruitment of tumor‐associated macrophages (TAMs) into the tumor microenvironment.
Pingping Gao, Na Sun, Ning Yang, Tingting Zhao, Rui Fu, Zhenyu Wu, Yuqin Zhou, Xuanni Tan, Yi Zhang, Fei Han, Xiaowei Qi   +10 more
wiley   +1 more source

Detection of Brazilian hantavirus by reverse transcription polymerase chain reaction amplification of N gene in patients with hantavirus cardiopulmonary syndrome

Memorias do Instituto Oswaldo Cruz, 2004
We report a nested reverse transcription-polymerase chain reaction (RT-PCR) assay for hantavirus using primers selected to match high homology regions of hantavirus genomes detected from the whole blood of hantavirus cardiopulmonary syndrome (HCPS ...
Marcos Lázaro Moreli, Ricardo Luiz Moro de Sousa, Luiz Tadeu Moraes Figueiredo   +2 more
doaj   +1 more source

Sensitivity test of the nested PCR assay.

, 2022
Ten-fold dilutions (1 ng to 10 fg) of C. siamense ML133 genomic DNA were used as the template. The nested PCR assay was performed using primers Col_nest-1F/Col_nest-1R for the first PCR, and Col_nest-2F/Col_nest-2R for the second PCR.
Chia-Lin Chung (815923), Ting-Hsuan Hung (304382), Hung-Yi Wu (409276), Yi-Chia Chen (5677163), Pei-Che Chung (12969231)   +4 more
core   +1 more source

FAM134B Restricts African Swine Fever Virus Capsid Assembly via Reticulophagy and Its Antiviral Activity is Antagonized by the Viral Virulence‐Associated Factor pMGF300‐2R

Advanced Science, EarlyView.
ABSTRACT African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating viral disease in domestic pigs and wild boar. ASFV is a large DNA virus whose capsid assembly takes place within perinuclear viral factories (VFs). Accumulating evidence has demonstrated that reticulophagy confers antiviral activity against multiple RNA ...
Rui Luo, Jing Zhang, Ruojia Huang, Xianfeng Zhang, Lian‐Feng Li, Jing Lan, Zhanhao Lu, Yuan Sun, Tao Wang, Hua‐Ji Qiu   +9 more
wiley   +1 more source

Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR

Indonesian Journal of Biotechnology, 2006
world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detecting dengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primers that conserve for several ...
Nastiti Wijayanti, Hera Nirwati, Tri Wibawa, Aris Haryanto, S. Sutaryo   +4 more
doaj   +1 more source

PCR primers used for nested PCR.

, 2015
Primers’ genome location: F1-14 492; R1-14 828; F2-14 528; R2-14 721; F3-1 642 737; R3-1 643 023; F4-1 642 764; R4-1 642 925; F5-2 935 537; R5-2 935 804; F6-2 935 594; R6-2 935 716PCR primers used for nested PCR.
Florence Brossier (803925), Emmanuelle Cambau (206542), Aurélie Chauffour (485000), Vincent Jarlier (243571), Alexandra Aubry (243572), Florence Reibel (636374)   +5 more
core   +1 more source