Results 121 to 130 of about 97,228 (263)

Maltase 1 regulates DENV2 infection and life history in Aedes aegypti

Insect Science, EarlyView.
Our study employed CRISPR/Cas9 to generate a MAL1 knockout strain of Aedes aegypti, demonstrating that the absence of MAL1 significantly suppresses DENV2 replication in the midgut and impairs viral transmission. The mutation also altered life‐history (hatching/pupation/emerging rates and sex‐specific survival), identifying MAL1 as a novel target for ...
Man‐Jin Li, Jin Cheng, Ya‐Ming Zou, Quan‐Chao Liu, Ding Zhu, Ce‐Jie Lan   +5 more
wiley   +1 more source

Nested PCR for diagnosing scabies infestation [PDF]

British Journal of Dermatology, 2018
J.E. Hahm, C.W. Kim, S.S. Kim
openaire   +1 more source

Co‐phylogeny and biogeography of the myrmecophilous beetle Paussus favieri (Carabidae, Paussinae) and its host ant Pheidole pallidula (Hymenoptera, Myrmicinae)

Insect Science, EarlyView.
Strict patterns of co‐divergence have rarely been documented other than among organisms and their symbionts. In this paper, using a molecular approach, we inferr the population‐level phylogenies of a Mediterranean ant species Pheidole pallidula and its nest parasite, the obligate myrmecophilous beetle Paussus favieri. We then investigate the role of co‐
Davide Bergamaschi, Wendy Moore, Andrea Di Giulio   +2 more
wiley   +1 more source

Diagnóstico temprano en un brote epidémico del virus Dengue en Piura usando RT-PCR y nested-PCR

Revista Peruana de Medicina Experimental y Salud Pública, 1997
Un test de diagnóstico temprano (RT-PCR y Nested-PCR) fue evaluado y comparado con métodos convencionales (cultivo in vitro, IFI y MAC-ELISA). Treinta y cuatro sueros de pacientes correspondientes de un brote epidémico de la costa norte peruana (Mancora,
Oscar Nolasco, Carlos Carrillo, Victoria Gutierrez, Carlos Yabar, Susan Douglas, María García, Ysabel Montoya   +6 more
doaj  

Development and application of an indirect ELISA and nested PCR for the epidemiological analysis of Klebsiella pneumoniae among pigs in China. [PDF]

Front Microbiol, 2023
Wu Z, Li N, Li Z, Wang J, Liu M, Qi M, Wei S, Wu T, Guo Y, Zhu J, Jiang H, Xue R, Sun C, Feng X, Gu J, Han W, Li F, Lei L.   +17 more
europepmc   +1 more source

Effects of Vector Density on Blood Parasite Spread and Health Consequences for Avian Hosts: An Experimental Epidemiology Blueprint

Integrative Zoology, EarlyView.
In an experimental epidemiology setup, groups of pigeons were exposed to different numbers of infective malaria vectors. Vector number explained the speed of transmission, the final prevalence, and changes in host behavior. ABSTRACT Vector density plays a critical role in the transmission dynamics of vector‐borne diseases and thus in their health and ...
Nayden Chakarov, Tim Brandler, Kai Fischer, Carmen Schwietz   +3 more
wiley   +1 more source

Unraveling Occurrence Patterns and Diversity of Avian Malaria Parasites in Iberian Obligate and Facultative Scavenger Birds

Integrative Zoology, EarlyView.
Low overall haemosporidian occurrence (3.4%; 13/383). Unexpected high lineage diversity (10 new host–parasite interactions). Novel Leucocytozoon lineage (GYPBAR01) in all European vultures except the griffon vulture. Plasmodium relictum (lineage SGS1) in adult red kites.
Pilar Oliva‐Vidal, Jaime Muriel, Alfonso Marzal, Grethell Mabel López del Aguila, José María Martínez, Antoni Margalida   +5 more
wiley   +1 more source

Nested-PCR [PDF]

, 2018
openaire   +2 more sources

Genomic Content in Avian Haemosporidian Parasites Suggests Co‐Regulation of Apicoplast and Mitochondrial Nucleoids

Integrative Zoology, EarlyView.
Apicoplast genomic content and mitochondrial genomic content were found to be strongly correlated (rho = 0.93) for infections going from low to high. Apicoplast and mitochondrial genomic content were deemed as more predictive factors of parasitemia for different infection intensities.
Gaia Porporato, Peter Pibaque, Alanis Cruz Flores, Simone Cescutti, Nayden Chakarov   +4 more
wiley   +1 more source

Domination Versus Sisterhoods in the Blood Microbiota of Migrating Birds: Patterns of Within‐ and Between‐Individual Blood Parasite Diversity Revealed Through Metabarcoding

Integrative Zoology, EarlyView.
Avian haemosporidian blood parasites are typically identified through Sanger sequencing of a partial cytochrome b fragment, the MalAvi barcoding region. Next‐generation sequencing is seldom used for avian blood parasite identification; this study demonstrates a higher detection rate of co‐infections via metabarcoding and its possible implications ...
Peter Pibaque, Gaia Porporato, Simone Cescutti, Alanis Cruz‐Flores, Tobias Busche, Anika Winker, Tim Maximilian Rapp, Patrick Bergkamp, Anna Doneva, Nayden Chakarov   +9 more
wiley   +1 more source