Establishment of Nested PCR for the Detection of Pseudomonas plecoglossicida and Epidemiological Survey of Larimichthys crocea in the Southeast Coastal Region. [PDF]
Duan X +6 more
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A Nested PCR Telomere Fusion Assay Highlights the Widespread End-Capping Protection of Arabidopsis CTC1. [PDF]
Vaquero-Sedas MI, Vega-Palas MA.
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Comparison of real-time PCR and nested PCR based on the HlyA gene for the detection of Listeria monocytogenes. Application on cheese samples. [PDF]
Zbrun MV +4 more
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Antimicrobial Susceptibility of Commensal Escherichia coli from Pig Fecal Samples and Enhanced Sensitivity for Direct Detection of the blaCTX-M Gene by Nested PCR. [PDF]
Suchanta N +4 more
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Molecular detection and phylogenetic analysis of Borrelia Spp. In blood samples of cats and dogs by the nested-PCR method in West Azerbaijan Province, Iran. [PDF]
Ownagh A +3 more
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The Use of the Internal Transcribed Spacer Region for Phylogenetic Analysis of the Microsporidian Parasite Enterocytozoon hepatopenaei Infecting Whiteleg Shrimp (Penaeus vannamei) and for the Development of a Nested PCR as Its Diagnostic Tool. [PDF]
Lee JH +12 more
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Nested Polymerase Chain Reaction (PCR)
Cold Spring Harbor Protocols, 2019Nested polymerase chain reaction (PCR) is used in situations in which it is necessary to increase the sensitivity and/or specificity of PCR, for example, when amplifying a particular member of a polymorphic gene family or when amplifying a cDNA copy of an mRNA present at very low abundance in a clinical specimen containing a heterogeneous population of
Michael R, Green, Joseph, Sambrook
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Multiplex nested PCR for Brazilian Alphavirus diagnosis
Transactions of the Royal Society of Tropical Medicine and Hygiene, 2004A reverse-transcriptase PCR (RT-PCR) and a multiplex nested PCR were developed for the rapid detection and identification of 14 Brazilian alphaviruses. Using Alphavirus genus-specific primers in a RT-PCR, we obtained amplified products of 434 bp. Species-specific primers were selected and simultaneously tested in a multiplex nested PCR.
Roberta V M, Bronzoni +3 more
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Nested PCR for the Detection of Cryptosporidium parvum
Parasitology Today, 1999In their recent review, Morgan and Thompson1xMorgan, U.M. and Thompson, R.C.A. Parasitol. Today. 1998; 14: 241–245Abstract | Full Text | Full Text PDF | PubMed | Scopus (48)See all References1 described the pitfalls of using nested PCR for the detection of the diarrhoea-causing protozoan, Cryptosporidium parvum, in faecal samples or in waste or surface
C L, Gibbons, F M, Awad-El-Kariem
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