Results 211 to 220 of about 186,282 (233)

Application of nested PCR for diagnosis of histoplasmosis

Journal of Infection and Chemotherapy, 2013
Histoplasmosis is a fungal infection that, although not endemic in Japan, has seen a rise in the number of Japanese cases since the mid-1980s. Diagnosis of the disease is not straightforward, and the main method of detection, fungal culture (which has biosafety-related issues), is of low sensitivity in general.
Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Yukihiro Kaneko, Satoshi Yamagoe, Yoshitsugu Miyazaki, Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Satoshi Yamagoe   +9 more
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Rapid HLA‐DRB1 genotyping by nested PCR amplification

Tissue Antigens, 1992
Abstract: State of the art genotyping of HLA class II alleles with group‐specific DNA amplification by the polymerase chain reaction (PCR) (1) and subsequent probing with sequence‐specific oligonucleotides (2–4) is not suitable for typing cadaveric organ donors since the typing procedure takes far more than one working day.
G, Bein, R, Gläser, H, Kirchner
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Specific Detection of Chikungunya Virus Using a RT‐PCR/Nested PCR Combination

Journal of Veterinary Medicine, Series B, 2002
Chikungunya (CHIK) virus is enzootic in many countries in Asia and throughout tropical Africa. In Asia the virus is transmitted from primates to humans almost exclusively by Aedes aegypti, while various aedine mosquito species are responsible for human infections in Africa.
M, Pfeffer, B, Linssen, M D, Parke, R M, Kinney   +3 more
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Schlussbericht "Nested on Chip PCR"

2009
Ill., graph. Darst.
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Detection of HPV Transcripts by Nested RT-PCR

2005
Human papillomaviruses (HPVs) are etiologic for the development of cervical cancer and its precursor lesions, cervical intraepithelial neoplasia (CIN). Nearly all cervical carcinomas (CaCx) harbor HPV DNA, but the presence of HPV alone is not indicative of the future development of neoplasia.
Christine, Mant, Barbara, Kell, John, Cason   +2 more
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Antisense PCR: A simple and robust method for performing nested single-tube PCR

Analytical Biochemistry, 2011
To overcome the disadvantages of two-round nested PCR, we developed a simple and robust closed single-tube nested PCR method (antisense PCR). The method uses antisense oligonucleotides that carry a 5' tag and that can potentially hybridize to the 3' ends of the outer primers, depending on the annealing temperature.
Michael J, Brisco, Paul A, Bartley, Alexander A, Morley   +2 more
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Nested PCR ve Kullanım Alanları

2013
Nested PCR, PCR tekniğinin hassasiyetini artırmak için geliştirilen en popular yöntemlerden biridir. İstenilen DNA dizisinin bulunup çoğaltılmasında hassasiyeti 104 kat artırdığı tahmin edilmektedir. Bu reaksiyon mekanizmasında birbirini takip eden iki polimeraz zincir reaksiyonunu içermektedir. İlk amplifikasyonda hedef DNA’nın dış bölgesine özgün iki
SEVİNDİK, Emre, ABACI, Z. Tuğba
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Lariat-Dependent Nested PCR for Flanking Sequence Determination

2010
Methods detailed in this chapter relate to the use of Lariat-dependent Nested (LaNe) PCR to characterize unknown RNA or DNA sequence flanking known regions. A multitude of approaches designed to determine flanking sequences have been described in the literature.
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Nested PCR

2011
BONIN, Serena, DOTTI, ISABELLA
openaire   +3 more sources

Nested PCR

2007
Opisana je metoda nested PCR koja se sastoji od dvije reakcije PCR u kojima se koriste dva različita para početnica.
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