Results 31 to 40 of about 36,040 (208)

Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

Nature Communications, 2022
Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently ...
Edward King, Sarah Maxel, Yulai Zhang, Karissa C. Kenney, Youtian Cui, Emma Luu, Justin B. Siegel, Gregory A. Weiss, Ray Luo, Han Li   +9 more
doaj   +1 more source

Nicotinamide mononucleotide and nicotinamide riboside attenuate cytokine production in human keratinocytes via suppression of p38 Pathway. [PDF]

Mol Biol Rep
BACKGROUND: Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by impaired epidermal barrier function and immune dysregulation.
Xie C, Velandia MAM, Marecek S, Khalil M, Hao J.   +4 more
europepmc   +2 more sources

The Wlds transgene reduces axon loss in a Charcot-Marie-Tooth disease 1A rat model and nicotinamide delays post-traumatic axonal degeneration

Neurobiology of Disease, 2011
Charcot-Marie-Tooth disease (CMT) is the most common inherited neuropathy and a duplication of the peripheral myelin protein of 22 kDa (PMP22) gene causes the most frequent subform CMT1A.
Gerd Meyer zu Horste, Timo A. Miesbach, Johanna I. Muller, Robert Fledrich, Ruth M. Stassart, Bernd C. Kieseier, Michael P. Coleman, Michael W. Sereda   +7 more
doaj   +1 more source

Comprehensive Transformation of Escherichia coli for Nicotinamide Mononucleotide Production

, 2023
Nicotinamide mononucleotide (NMN) is a key precursor of nicotinamide adenine dinucleotide and an important source of cellular energy. It can prevent neuronal mitochondrial defects and alleviate heart fibrosis.
Linyan Yang, Tianjiao Bi, Tao Wu, Yan Xu, Xiaoqing Mu   +4 more
core   +1 more source

Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform

Nature Communications, 2022
Engineering enzymes to utilize the noncanonical redox cofactors such as nicotinamide mononucleotide (NMN + ) is challenging. Here, the authors report a growth-based selection platform for NMN + -reducing enzyme engineering and show its application in ...
Linyue Zhang, Edward King, William B. Black, Christian M. Heckmann, Allison Wolder, Youtian Cui, Francis Nicklen, Justin B. Siegel, Ray Luo, Caroline E. Paul, Han Li   +10 more
doaj   +1 more source

Engineering a nicotinamide mononucleotide redox cofactor system for biocatalysis. [PDF]

, 2020
Biological production of chemicals often requires the use of cellular cofactors, such as nicotinamide adenine dinucleotide phosphate (NADP+). These cofactors are expensive to use in vitro and difficult to control in vivo.
Cui, Youtian, Fong, Bonnie, Maxel, Sarah, King, Edward, Siegel, Justin B, Li, Han, Zhang, Linyue, Mak, Wai Shun, Black, William B, Sanchez Martinez, Alicia   +9 more
core   +1 more source

Effects of NAD+ precursor supplementation on glucose and lipid metabolism in humans: a meta-analysis

Nutrition & Metabolism, 2022
Background This meta-analysis was performed to investigate the effects of nicotinamide adenine dinucleotide (NAD+) precursor supplementation on glucose and lipid metabolism in human body.
Ou Zhong, Jinyuan Wang, Yongpeng Tan, Xiaocan Lei, Zhihan Tang   +4 more
doaj   +1 more source

Nicotinamide mononucleotide ameliorates adriamycin-induced renal damage by epigenetically suppressing the NMN/NAD consumers mediated by Twist2

Scientific Reports, 2022
The activation of nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, Sirt1, after the administration of nicotinamide mononucleotide (NMN) suppresses many diseases. However, the role of NMN and Sirt1 in focal glomerulosclerosis (FSGS) has not
Kazuhiro Hasegawa, Yusuke Sakamaki, Masanori Tamaki, Shu Wakino   +3 more
doaj   +1 more source

Fluorescent cAMP Analogs: Enzymatic Synthesis, Unexpected Photophysical Behavior and Protein Kinase A Recognition

Angewandte Chemie, EarlyView.
Cyclic adenosine monophosphate (cAMP) is a key cellular second messenger. Adenylyl cyclase converts emissive isomorphic nucleoside triphosphates into fluorescent cAMPs. cthAMP displays unusually high brightness compared to thATP, its precursor, enabling real‐time monitoring of enzymatic reactions.
Deyuan Cong, Victor Dubois, Jamie Y. Lam, Yitzhak Tor   +3 more
wiley   +2 more sources

Mononucleotide repeats are asymmetrically distributed in fungal genes [PDF]

, 2008
Background Systematic analyses of sequence features have resulted in a better characterisation of the organisation of the genome. A previous study in prokaryotes on the distribution of sequence repeats, which are notoriously variable and can disrupt the ...
Graaff, L.H., de, de Graaff, L.H., van Passel Mark WJ, Passel, M.W.J., van, Mark WJ van Passel, Leo H de Graaff, van Passel, M.W.J., de Graaff Leo H   +7 more
core   +1 more source