Results 91 to 100 of about 7,960 (193)
Functional Analysis of Transmembrane Domain 3 in NKCC1 [PDF]
Biophysical Journal, 2011 We sought to determine the functional roles of residues in transmembrane domain (TM) 3 of human NKCC1 using tryptophan and cysteine scanning mutagenesis. We generated a structural alignment of the transmembrane domains of NKCC1 to the related APC transporters ApcT and AdiC, and obtained 3-D alignments using Modeller. Based on these alignments, residues
Somasekharan, Suma, Forbush, Biff
openaire +1 more source
Seizure-induced alterations in NKCC1 and KCC2 protein levels.
, 2013 Using Western Blot, NKCC1 and KCC2 protein levels were assessed in cortical and hippocampal tissue obtained from P10 rats at 1, 12, 24, 48, and 168 hr after hypoxia-induced seizures (HS), and compared to age-matched littermate controls. Optical densities
Kristopher T. Kahle (337479) +11 more
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Glia, Volume 74, Issue 9, September 2026.Mechanism of synthesis, release, receptor activation, uptake, and degradation of GABA in dorsal root ganglia (DRG). Left: GABA is synthesized from putrescine in satellite glial cells (SGCs). After being released by Best1 channel, it activates GABAA receptors in sensory neurons, producing a chloride current.
Natalie Jiménez‐Barrios +7 more
wiley +1 more source
Confocal immunofluorescence images of NKCC1 cell lines.
, 2013 After fixation, NKCC1 is visualized using anti-HA-antibody (HA-hNKCC1) or anti FLAG-antbody (green, Alexa 488 secondary). Nuclei are stained with TO-PRO-3-iodide (Life Technologies). Shown are HA-tagged hNKCC1 [9], 3xFlag-YFP-tagged hNKCC1 (NT17), 3xFlag-
Michelle Y. Monette (493120) +2 more
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Integrative simulation analysis of myocardial ischaemia–reperfusion injury
The Journal of Physiology, Volume 604, Issue 18, Page 7640-7662, 15 September 2026.Abstract figure legend A comprehensive mathematical model of ventricular myocyte was developed to gain insights into the mechanisms underlying cardiac ischaemia–reperfusion injury. The model integrates excitation–contraction (E–C) coupling, cytosolic and mitochondrial pH regulation, pH dependence of major E–C coupling components and cellular energy ...
Takao Shimayoshi +2 more
wiley +1 more source
Mapping Trofinetide Polypharmacology in Rett Syndrome: A Multi‐Stage Computational Analysis
Journal of Computational Chemistry, Volume 47, Issue 21, August 5, 2026.How can a single molecule address the complex symptoms of Rett syndrome? This computational study maps the multi‐target mechanism of trofinetide, revealing diverse modes of binding across five key receptors: GAT1, GABAA, CHRM1, AMPA, and GSK3β. From deep orthosteric anchoring to dynamic surface interactions, these structural insights provide a rational
Luis Felipe Hernández‐Ayala +2 more
wiley +1 more source
Role of Monovalent Ions in the NKCC1 Inhibition Mechanism Revealed through Molecular Simulations
, 2022 The secondary active Na-K-Cl cotransporter 1 (NKCC1) promotes electroneutral uptake of two chloride ions, one sodium ion and one potassium ion. NKCC1 regulates Cl(−) homeostasis, thus being implicated in transepithelial water transport and in neuronal ...
Janoš, Pavel +3 more
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Research progress on Na+ ⁃ K+ ⁃ 2Cl- cotransporter 1 and its relationship to nervous system diseases
Chinese Journal of Contemporary Neurology and Neurosurgery, 2021 Na+ ⁃ K+ ⁃ 2Cl- cotransporter 1 (NKCC1) which plays an important role in the dynamic balance of Cl- ions, is one of the cation⁃chloride cotransporter. It is a membrane protein that transferred the mediates Na+ , K+ and Cl- .
FU Xu⁃yang +3 more
doaj
Acute Hyperglycemia Aggravates Lung Injury via Activation of the SGK1–NKCC1 Pathway
, 2020 Acute lung injury (ALI) is characterized by severe hypoxemia and has significantly high mortality rates. Acute hyperglycemia occurs in patients with conditions such as sepsis or trauma, among others, and it results in aggravated inflammation and induces ...
Chin-Pyng Wu +3 more
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, 2019 Hypoxic-ischemic encephalopathy (HIE) is a serious birth complication with severe long-term sequelae such as cerebral palsy, epilepsy and cognitive disabilities. Na+-K+-2Cl– cotransporters 1 (NKCC1) is dramatically upregulated after hypoxia-ischemia (HI),
Lin Shao (242141) +10 more
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