Results 101 to 110 of about 7,960 (193)

Effect of diclofenac on NKCC1 activity in T84 cells.

, 2014
(A) Diagrammatic protocol for Tl+ influx-based assay of NKCC1 activity. After loading with Tl+-sensitive dye, T84 cells were incubated for 15 min in the Cl−-free buffer containing Tl2SO4 and clotrimazole (to block K+ channels) with or without diclofenac (
Pawin Pongkorpsakol (625649), Potjanee Srimanote (122134), Chatchai Muanprasat (285849), Nutthapoom Pathomthongtaweechai (285853), Sunhapas Soodvilai (388059), Varanuj Chatsudthipong (388060)   +5 more
core   +1 more source

¬Immunolocalization of cation-chloride cotransporters in the developing and mature spinal cord of opossums, Monodelphis domestica

Frontiers in Neuroanatomy, 2013
Spinal inhibition is required to generate coordinated outputs between antagonistic muscles during locomotion. It relies on low neuronal chloride concentration set by two cation-chloride cotransporters, NKCC1 and KCC2 which, respectively, pumps Cl- in or ...
Ha-Loan ePhan, Jean-François ePflieger
doaj   +1 more source

Elevated intracellular chloride level in albino visual cortex neurons is mediated by Na-K-Cl co-transporter

BMC Neuroscience, 2008
Background During development the switch from a depolarizing to a hyperpolarizing action of GABA is a consequence of a decrease of the Na+-K+-2Cl- co-transporter (NKCC1, Cl--uptake) and increase of the K+-Cl- co-transporter (KCC2, Cl--extrusion ...
Turchinovich Andrey, Diykov Dmitry, Zoidl Georg, Hoffmann Klaus-Peter   +3 more
doaj   +1 more source

Effect of E289G-like mutations in KCC2 and NKCC1.

, 2013
K+ influx was measured through unidirectional 86Rb tracer uptake in oocytes injected with water, wild-type KCC2 or KCC2-E201G or KCC2-E201D mutant cRNAs, and wild-type or NKCC1-E383G or NKCC1-E383D mutant cRNAs. Uptakes were measured under isosmotic (200
Jinlong Ding (399034), José Ponce-Coria (399035), Eric Delpire (399036)   +2 more
core   +1 more source

Cab39 modulates SPAK/OSR1 activation of NKCC1

The FASEB Journal, 2013
Calcium binding protein 39 (Cab39) is a scaffolding protein that stabilizes a heterotrimeric protein complex that modulates the activity of the adenosine mono‐phosphate kinase which regulates cellular energy homeostasis. Cab39 also modulates SPAK (Ste20 related Proline Alanine rich Kinase) and OSR1 (Oxidative stress responsive ...
José Ponce‐Coria, Kerri Rios, Kenneth B. Gagnon, Eric Delpire   +3 more
openaire   +1 more source

NKCC1: tales from the dark side of the crypt [PDF]

The Journal of Physiology, 2007
Intestinal electrolyte secretion consist of several fundamental components: a basolateral uptake pathway, identified as the Na+–K+–Cl− cotransporter (NKCC1) (Payne et al. 1995) and an apical secretory pathway. NKCCs are highly conserved across organ and species, serving essential ion secretory and absorptive functions. Distinct regulatory mechanisms
openaire   +1 more source

Structure and Function of the Transporter NKCC1 [PDF]

Biophysical Journal, 2018
Corinne Portioli, Marco De Vivo, Ming Zhou, Laura Cancedda   +3 more
openaire   +1 more source

Dynamic and differential regulation of NKCC1 by calcium and cAMPin the native human colonic epithelium

, 2007
The capacity of the intestine to secrete fluid is dependent on the basolateral Na+-K+-2Cl(-) co-transporter (NKCC1). Given that cAMP and Ca2+ signals promote sustained and transient episodes of fluid secretion, respectively, this study investigated the ...
Sharp, Paul, Sharp, Paul; id_orcid, Tighe, Richard, Parris, Alyson, Lewis, Michael, Williams, Mark R., Evans, Luke A., Reynolds, Amy, Lindqvist, Susanne   +8 more
core   +1 more source

NKCC1 (SLC12a2) induces a secondary axis in Xenopus laevis embryos independently of its co-transporter function

, 2009
NKCC1 is a broadly expressed Na(+)-K(+)-Cl(-) co-transporter involved in regulation of ion flux across the cell membrane and in regulating cell volume. Whilst much is known about the co-transporter activity of NKCC1 and its regulation by protein kinases ...
Latinkic, Branko V., Haworth, Kim E., Latinkic, Branko, Haworth, Kim, Walters, Zoë S.   +4 more
core   +1 more source

Mapping NKCC1 in the Endocytic Pathway During PKC Activation in Mardin Darby Canine Kidney Cells

, 2018
Gut clearance represents an important defense mechanism of the gut by flushing out luminal bacteria and toxins. Transepithelial chloride fluid secretion is what drives gut clearance.
Klosa, Payton, Bazaldua, Amanda, Jones, Chase, Hughes, Emily, Petreska, Natasa, Barron, Nicholas, Bouyer, Patrice G, Dr., Dix, Marie   +7 more
core   +1 more source