Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target [PDF]
, 2010 Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Lee, TV +11 more
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An Engineered Nonribosomal Peptide Synthetase Shows Opposite Amino Acid Loading and Condensation Specificity [PDF]
, 2021 Engineering of nonribosomal peptide synthetases (NRPS) has faced numerous obstacles despite being an attractive path towards novel bioactive molecules. Specificity filters in the nonribosomal peptide assembly line determine engineering success, but the
Philipp, Stephan +5 more
core +2 more sources
Nonribosomal Peptide Synthesis
, 2022 Nonribosomal peptides (NRPs) are a type of secondary metabolite with a wide range of pharmacological and biological activities including cytostatics, immunosuppressants or anticancer agents, antibiotics, pigments, siderophores, toxins. NRPs, unlike other proteins, are synthesized on huge nonribosomal peptide synthetase (NRPS) enzyme complexes that are ...
Sadık Dincer +2 more
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Nonribosomal Peptide Synthesis and Toxigenicity of Cyanobacteria [PDF]
Journal of Bacteriology, 1999 ABSTRACT Nonribosomal peptide synthesis is achieved in prokaryotes and lower eukaryotes by the thiotemplate function of large, modular enzyme complexes known collectively as peptide synthetases. These and other multifunctional enzyme complexes, such as polyketide synthases, are of interest due to their use in unnatural-product or ...
B A, Neilan +6 more
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Novel chemical probes for the investigation of nonribosomal peptide assembly [PDF]
, 2017 Chemical probes were devised and evaluated for the capture of biosynthetic intermediates involved in the bio-assembly of the nonribosomal peptide echinomycin. Putative intermediate peptide species were isolated and characterised, providing fresh insights
Manuela Tosin +29 more
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Noncanonical Peloruside A Biosynthesis by an Uncultivated Verrucomicrobiota Symbiont
Angewandte Chemie, EarlyView.Noncanonical biochemistry of the peloruside PKS characterized in this work, comprising double bond generation by a noncanonical tridomain (blue), acetyl‐CoA recycling (orange), and proofreading systems (purple and yellow). The Z‐double bond is installed by TEB1 through O‐acetylation and acetate elimination. The acyl ligase PelB (AL, orange) selectively
Amy E. Fraley +11 more
wiley +2 more sources
Rapid Peptide Cyclization Inspired by the Modular Logic of Nonribosomal Peptide Synthetases. [PDF]
J Am Chem SocNonribosomal cyclic peptides (NRcPs) are structurally complex natural products and a vital pool of therapeutics, particularly antibiotics. Their structural diversity arises from the ability of the multidomain enzyme assembly lines, nonribosomal peptide ...
Ding Y +12 more
europepmc +2 more sources
Resurrecting ancestral antibiotics: unveiling the origins of modern lipid II targeting glycopeptides
Nature Communications, 2023 Antibiotics are central to modern medicine, and yet they are mainly the products of intra and inter-kingdom evolutionary warfare. To understand how nature evolves antibiotics around a common mechanism of action, we investigated the origins of an ...
Mathias H. Hansen +8 more
doaj +1 more source
Major Reference Works, Page 1-45., 2018 Bergey's Manual of Systematics of Archaea and Bacteria (BMSAB) is a reference work aimed at undergraduates, graduate students, researchers, professors and experienced professionals at all levels. About a hundred new genera and 600+ new species have been described per year for each of the last 5 years.
Peter Vandamme, Leo Eberl
wiley +1 more source
Molecular Plant-Microbe Interactions, 2006 Cyclic lipopeptides (CLPs) are versatile molecules produced by a variety of bacterial genera, including plant-associated Pseudomonas spp. CLPs are composed of a fatty acid tail linked to a short oligopeptide, which is cyclized to form a lactone ring ...
Jos M. Raaijmakers +2 more
doaj +1 more source