Results 21 to 30 of about 494,636 (171)

PARAS: High-Accuracy Machine Learning of Substrate Specificities in Nonribosomal Peptide Synthetases. [PDF]

JACS Au
International audienceNonribosomal peptides are chemically and functionally diverse natural products with important applications in medicine and agriculture.
Terlouw BR, Huang C, Meijer D, Cediel-Becerra JDD, He R, Rothe ML, Jenner M, Zhou S, Zhang Y, Fage CD, Tsunematsu Y, van Wezel GP, Robinson SL, Alberti F, Alkhalaf LM, Chevrette MG, Challis GL, Medema MH.   +17 more
europepmc   +3 more sources

A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum. [PDF]

PLoS ONE, 2014
The filamentous fungus Penicillium chrysogenum harbors an astonishing variety of nonribosomal peptide synthetase genes, which encode proteins known to produce complex bioactive metabolites from simple building blocks. Here we report a novel non-canonical
Hazrat Ali, Marco I Ries, Peter P Lankhorst, Rob A M van der Hoeven, Olaf L Schouten, Marek Noga, Thomas Hankemeier, Noël N M E van Peij, Roel A L Bovenberg, Rob J Vreeken, Arnold J M Driessen   +10 more
doaj   +1 more source

Exploring the selectivity and engineering potential of an NRPS condensation domain involved in the biosynthesis of the thermophilic siderophore fuscachelin

Frontiers in Catalysis, 2023
In nonribosomal peptide synthesis, condensation (C) domains are key catalytic domains that most commonly link carrier protein bound substrates to form peptides or depsipeptides.
Y. T. Candace Ho, Y. T. Candace Ho, Y. T. Candace Ho, Y. T. Candace Ho, Thierry Izoré, Thierry Izoré, Joe A. Kaczmarski, Joe A. Kaczmarski, Edward Marschall, Edward Marschall, Edward Marschall, Minuri S. Ratnayake, Minuri S. Ratnayake, Minuri S. Ratnayake, Julien Tailhades, Julien Tailhades, Julien Tailhades, David L. Steer, David L. Steer, Ralf B. Schittenhelm, Ralf B. Schittenhelm, Manuela Tosin, Colin J. Jackson, Colin J. Jackson, Colin J. Jackson, Colin J. Jackson, Max J. Cryle, Max J. Cryle, Max J. Cryle   +28 more
doaj   +1 more source

Expanding the toolbox for engineering nonribosomal peptide synthetases [PDF]

, 2023
Nonribosomal peptide synthetases (NRPSs) are an important source of pharmaceutically valuable natural products, including antibiotics. In depth understanding of enzymatic interactions responsible for the biosynthesis of these compounds by NRPSs is ...
Pourmasoumi, Farzaneh
core   +1 more source

Biosynthesis and engineering of the nonribosomal peptides with a C-terminal putrescine

Nature Communications, 2023
The broad bioactivities of nonribosomal peptides rely on increasing structural diversity. Genome mining of the Burkholderiales strain Schlegelella brevitalea DSM 7029 leads to the identification of a class of dodecapeptides, glidonins, that feature ...
Hanna Chen, Lin Zhong, Haibo Zhou, Xianping Bai, Tao Sun, Xingyan Wang, Yiming Zhao, Xiaoqi Ji, Qiang Tu, Youming Zhang, Xiaoying Bian   +10 more
doaj   +1 more source

An Engineered Nonribosomal Peptide Synthetase Shows Opposite Amino Acid Loading and Condensation Specificity [PDF]

, 2021
Engineering of nonribosomal peptide synthetases (NRPS) has faced numerous obstacles despite being an attractive path towards novel bioactive molecules. Specificity filters in the nonribosomal peptide assembly line determine engineering success, but the
Philipp, Stephan, David L., Niquille, Aleksa, Stanišić, Annika, Hüsken, Jochen, Reinstein, Hajo, Kries   +5 more
core   +2 more sources

Beyond Toxin Transport: Novel Role of ABC Transporter for Enzymatic Machinery of Cereulide NRPS Assembly Line

mBio, 2020
Nonribosomal peptide synthetases (NRPSs) and polyketide synthetases (PKSs) play a pivotal role in the production of bioactive natural products, such as antibiotics and cytotoxins. Despite biomedical and pharmaceutical importance, the molecular mechanisms
A. Gacek-Matthews, Z. Chromiková, M. Sulyok, G. Lücking, I. Barák, M. Ehling-Schulz   +5 more
doaj   +1 more source

Classification and Secondary Metabolite-Biosynthetic Gene Clusters of Marine Streptomyces Strains Including a Lobophorin- and Divergolide-Producer

Hydrobiology, 2023
Two Streptomyces strains, named N11-26 and DC10-5, were isolated from deep-sea and non-photosynthetic stony coral, respectively. Strain N11-26 produces lobophorin C and divergolides, which are antimicrobial substances.
Hisayuki Komaki, Tomohiko Tamura, Yasuhiro Igarashi   +2 more
doaj   +1 more source

Antibacterial Property Of A Coral-Associated Bacterium Pseudoalteromonas Luteoviolacea Against Shrimp Pathogenic Vibrio Harveyi(In Vitro Study) [PDF]

, 2004
A coral-associated bacterium was successfully screened for secondary metabolites production based on PCR amplification of the non-ribosomal peptide synthetase gene and was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S ...
Grossart, Hans-Peter, Sabdono, Agus, Bachtiar, Tonny, Karna Radjasa, Ocky, Marten, Torben, Simon, Meinhard   +5 more
core   +2 more sources

Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target [PDF]

, 2010
Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Lee, TV, Arcus, VL, Lott, JS, Johnson, RD, Lott, J. Shaun, Vickery L. Arcus, Johnson, Richard D., Arcus, Vickery L., J. Shaun Lott, Richard D. Johnson, T. Verne Lee, Lee, T. Verne   +11 more
core   +1 more source