Results 81 to 90 of about 9,796 (161)

Differences in the suitability of published honey bee (Apis mellifera) reference genes between the African subspecies Apis mellifera scutellata and European derived Apis mellifera

Molecular Ecology, Volume 34, Issue 15, August 2025.
Abstract Quantitative real‐time polymerase chain reaction (qPCR) is a method widely used to determine changes and differences in gene expression. As target gene expression is most often quantified relative to the expression of reference genes, the validation of suitable reference genes is of critical importance.
Anja Buttstedt, Christian W. W. Pirk, Abdullahi A. Yusuf   +2 more
wiley   +1 more source

Identification of stable reference genes for quantitative PCR in koalas [PDF]

, 2018
To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation.
A Kappel, A Radonic, B Artegiani, CA Waugh, CL Andersen, D Kim, DG Ginzinger, F Almeida-Oliveira, F Bartz, G Bamias, GS Meers, GS Simmons, IE Maher, IE Maher, J Vandesompele, J Woinarski, J Yperman, JJ Hanger, K Ahn, KC Thomas, KM Morris, M Kubista, M Pertea, MA Valasek, MW Pfaffl, N Silver, OT Ong, P Brym, PD Lee, PJ Canfield, QL Zhang, R Tarlinton, R Tarlinton, RK Das, S Bages, SA Bustin, SA Bustin, T Burmeister, T Nolan, V Gonzalez-Astudillo, Y Zhang   +40 more
core   +4 more sources

Differential changes in anatomical, molecular and functional determinants of intestinal glucose absorption during murine pregnancy

Physiological Reports, Volume 13, Issue 15, August 2025.
Abstract Pregnancy demands increased food intake and nutrient delivery to support the growing conceptus. The timing and regional specificity of adaptations in small intestinal (SI) anatomy and transport, and their functional consequences, remain unclear. We therefore assessed anatomy and expression of macronutrient transporters in the duodenum, jejunum,
T. Sebastian Overduin, Georgia S. Clarke, Hui Li, Richard L. Young, Kathryn L. Gatford, Amanda J. Page   +5 more
wiley   +1 more source

Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Journal of Experimental & Clinical Cancer Research, 2010
Background Real-time reverse transcription PCR (qRT-PCR) is frequently used for gene expression quantification due to its methodological reproducibility and sensitivity.
Andersen Solveig N, Sørby Lise, Bukholm Ida RK, Jacobsen Morten B   +3 more
doaj   +1 more source

Gas Bubbles from Biodegradable Magnesium Implants Convey Mechanical Cues and Promote Immune Cell Stimulation

Advanced Science, Volume 12, Issue 28, July 24, 2025.
Bubbles in soft tissue are the unavoidable consequence of magnesium implant degradation in patients, yet their biological significance remains unclear. Using advanced imaging and spatial transcriptomics, this study reveals a pronounced inflammatory and mechanical response near bubbles, contrasting with pro‐regenerative events closer to the implant ...
Heithem Ben Amara, Jincy Philip, Omar Omar, Peter Thomsen   +3 more
wiley   +1 more source

Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia [PDF]

, 2016
BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug ...
Brian Dean, Elizabeth Scarr, Madhara Udawela   +2 more
core   +4 more sources

High sensitivity to ocean acidification in wild out‐migrating juvenile Pacific salmon is not impacted by feeding success

Ecological Applications, Volume 35, Issue 5, July 2025.
Abstract Salmon populations are declining worldwide, with high mortality rates during juvenile marine migration presenting a bottleneck to recruitment. The ocean conditions along the main migratory route of juvenile salmon in British Columbia are characterized by high variability in CO2, with the amplitude, duration, and frequency of ocean ...
Andrea Y. Frommel, Arash Akbarzadeh, Virginie Chalifoux, Tobi J. Ming, Brenna Collicutt, Kate Rolheiser, Rumer Opie, Kristina M. Miller, Colin J. Brauner, Brian P. V. Hunt   +9 more
wiley   +1 more source

Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage

Journal of Animal Science and Biotechnology, 2012
Background Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results.
McCulloch Ryan S, Ashwell Melissa S, O’Nan Audrey T, Mente Peter L   +3 more
doaj   +1 more source

Stable reference genes for the measurement of transcript abundance during larval caste development in the honeybee [PDF]

, 2013
Many genes are differentially regulated by caste development in the honeybee. Identifying and understanding these differences is key to discovering the mechanisms underlying this process.
A Lourenco, A Patel, AR Barchuk, B Scharlaken, CL Andersen, DE Wheeler, DW Severson, Elizabeth J. Duncan, F Wolschin, FC Humann, G-H Wang, GV Glass, H Holzl, H Stabe, J Hellemans, J Vandesompele, JD Evans, JD Evans, LM Lix, M Boer de, M Corona, M Okoniewski, M Sardiello, MA Valasek, MW Pfaffl, NS Mutti, Peter K. Dearden, R Kucharski, Rosannah C. Cameron, S Rozen, SA Bustin, SV Azevedo, SV Azevedo de, X Chen   +33 more
core   +3 more sources

Classification and Prognostication of B‐Cell and T‐Cell Multicentric Lymphoma in Dogs Using Serum MicroRNAs

Veterinary and Comparative Oncology, Volume 23, Issue 2, Page 310-319, June 2025.
ABSTRACT Canine multicentric lymphoma is a common malignancy in dogs. It often responds well to initial chemotherapy but frequently relapses and has a poor response to subsequent treatment. B‐cell (BCL) and T‐cell (TCL) lymphomas differ in both their prognoses and chemotherapeutic treatment protocols.
Latasha Ludwig, Heather Treleaven, Roger Moorehead, Robert A. Foster, R. Darren Wood, R. Ayesha Ali, Geoffrey A. Wood   +6 more
wiley   +1 more source