Results 81 to 90 of about 9,815 (163)

Beet Chlorosis Virus Infection Mitigates Aphid‐Induced Plant Defences and Improves Plant Acceptability to Aphid Vectors

Molecular Ecology, Volume 34, Issue 20, October 2025.
ABSTRACT Plant viruses often alter host traits in ways that affect interactions with herbivores, potentially facilitating their own acquisition and transmission by insect vectors. However, little is known about the molecular mechanisms underlying this phenomenon.
Thomas Armand, Sylvaine Boissinot, Alessandra Maia‐Grondard, Philippe Hugueney, Véronique Brault, Quentin Chesnais   +5 more
wiley   +1 more source

Selection of reference genes for quantitative RT-PCR studies in striped dolphin () skin biopsies [PDF]

, 2006
Background: Odontocete cetaceans occupy the top position of the marine food-web and are particularly sensitive to the bioaccumulation of lipophilic contaminants.
SPINSANTI, G., PANTI, C., LAZZERI, E., MARSILI, L., CASINI, S., FRATI, F., FOSSI, M. C.   +6 more
core   +1 more source

Epithelial Memory After Respiratory Viral Infection in Mice Results in Prolonged Enhancement of Antigen Presentation

Allergy, Volume 80, Issue 9, Page 2501-2518, September 2025.
RSV infection induces lasting epigenetic and transcriptional changes in LECs, upregulating MHC‐I and MHC‐II, further amplified by LPS exposure. This is accompanied by increased antigen uptake, processing, and presentation. These persistent alterations may influence immune responses, potentially impacting both immunity to infection and respiratory ...
Piotr P. Janas, Wouter T'Jonck, Matthew O. Burgess, Maximilian Reck, Caroline Chauché, Matthieu Vermeren, Christopher D. Lucas, Calum Bain, Robert Illingworth, Edward W. Roberts, Henry J. McSorley, Jürgen Schwarze   +11 more
wiley   +1 more source

Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Journal of Experimental & Clinical Cancer Research, 2010
Background Real-time reverse transcription PCR (qRT-PCR) is frequently used for gene expression quantification due to its methodological reproducibility and sensitivity.
Andersen Solveig N, Sørby Lise, Bukholm Ida RK, Jacobsen Morten B   +3 more
doaj   +1 more source

Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors [PDF]

, 2013
BACKGROUND: Quantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls is critical to normalise the results, thereby avoiding differences and ...
Astudillo, Aurora, Escudero, Dolores, Gómez, Enrique, Otero, Jesús, Pérez, Silvia, Rodríguez, Aida, Royo, L.J. (Luis), Álvarez, Francisco   +7 more
core  

microRNAs for qPCR Normalization Under Morphofunctional Conditions in Bovine Sperm (Bos taurus)

Molecular Reproduction and Development, Volume 92, Issue 8, August 2025.
Identification of the most stable microRNAs in Bos taurus semen for qPCR analysis under sperm motility and morphology conditions. ABSTRACT Cattle represents one of the most common and widely distributed categories of large ruminants, with well‐established production practices.
Lucas Petitemberte de Souza, Leandro Silva Nunes, Luana Carla Salvi, Laís dos Santos Gonçalves, Luana Ferreira Viana dos Reis, Izani Bonel Acosta, Carine Dahl Corcini, Antonio Sergio Varela Junior, Fábio Gularte Barreto, Marcelo Brandi Vieira, Diego Corrêa Silveira, Jeaniffer Melgarejo Vieira, Gustavo Freitas Ilha, William Borges Domingues, Vinicius Farias Campos   +14 more
wiley   +1 more source

18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells [PDF]

, 2012
Background: One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection.
Baquero Perez, Belinda, Brookes, Sharon M., Brown, Ian H., Chang, Kin-Chow, Dunham, Stephen P., Kuchipudi, Suresh V., Nelli, Rahul K., Sebastian, Sujith, Slomka, Marek J., Tellabati, Meenu, White, Gavin A.   +10 more
core   +1 more source

Differences in the suitability of published honey bee (Apis mellifera) reference genes between the African subspecies Apis mellifera scutellata and European derived Apis mellifera

Molecular Ecology, Volume 34, Issue 15, August 2025.
Abstract Quantitative real‐time polymerase chain reaction (qPCR) is a method widely used to determine changes and differences in gene expression. As target gene expression is most often quantified relative to the expression of reference genes, the validation of suitable reference genes is of critical importance.
Anja Buttstedt, Christian W. W. Pirk, Abdullahi A. Yusuf   +2 more
wiley   +1 more source

Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage

Journal of Animal Science and Biotechnology, 2012
Background Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results.
McCulloch Ryan S, Ashwell Melissa S, O’Nan Audrey T, Mente Peter L   +3 more
doaj   +1 more source

Identification of stably expressed reference small non-coding RNAs for microRNA quantification in high-grade serous ovarian carcinoma tissues [PDF]

, 2016
MicroRNAs (miRNAs) belong to a family of small non‐coding RNAs (sncRNAs) playing important roles in human carcinogenesis. Multiple investigations reported miRNAs aberrantly expressed in several cancers, including high‐grade serous ovarian carcinoma (HGS ...
Bandiera, Elisabetta, Bignotti, Eliana, Calza, Stefano, Odicino, Franco., Ravaggi, Antonella, Romani, Chiara, Sartori, Enrico, Tassi, Renata A., Todeschini, Paola, Zanotti, Laura   +9 more
core   +1 more source