Results 51 to 60 of about 1,883 (170)
Stable transduced hCDC-nSMase2 lentiviral knockdown lines block exosome secretion.
, 2016 (A) Bradford assay and (B) immunoblot analysis of CD63 of exosomes from CDCs transduced with lentiviral vectors for scrambled or nSMase2-specific shRNAi show nSMase2 knockdown results in significantly less release of exosomes from human CDCs (n = 3 ...
Jennifer K. Lang (344441) +3 more
core +1 more source
Neutral Sphingomyelinase 2 (nSMase2)-dependent Exosomal Transfer of Angiogenic MicroRNAs Regulate Cancer Cell Metastasis [PDF]
Journal of Biological Chemistry, 2013 The release of humoral factors between cancer cells and the microenvironmental cells is critical for metastasis; however, the roles of secreted miRNAs in non-cell autonomous cancer progression against microenvironmental cells remain largely unknown. Here, we demonstrate that the neutral sphyngomyelinase 2 (nSMase2) regulates exosomal microRNA (miRNA ...
Kosaka, Nobuyoshi +5 more
openaire +2 more sources
Journal of Extracellular VesiclesExtracellular vesicles (EVs) are shed from the plasma membrane, but the regulation and function of these EVs remain unclear. We found that oxidative stress induced by H2O2 in Hela cells stimulated filopodia formation and the secretion of EVs.
Zainuddin Quadri +10 more
doaj +1 more source
MedComm, Volume 7, Issue 10, October 2026.• EV content maintains cellular homeostasis and is involved in several disease onset and progression. • miRNA export in EVs depends on: RNA‐binding proteins recognizing motif sequences and on epitranscriptomic modifications. • The disclosure of delivery mechanisms is crucial for developing personalized EV‐based therapeutic tools. ABSTRACT Extracellular
Sabrina Garbo +4 more
wiley +1 more source
The hyaluronic acid inhibitor 4-methylumbelliferone is an NSMase2 activator—role of Ceramide in MU anti-tumor activity [PDF]
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2016 Increased synthesis of hyaluronic acid (HA) is often associated with increased metastatic potential and invasivity of tumor cells. 4-Methylumbelliferone (MU) is an inhibitor of HA synthesis, and has been studied as a potential anti-tumor drug to inhibit the growth of primary tumors and distant metastasis of tumor cells.
Jingdong, Qin +2 more
openaire +2 more sources
Characterization of fluorescence based activity assay for human nSMase2.
, 2015 Plots show the dependence of enzymatic activity with respect to (A) protein concentration in the presence of 250 μM SM for 1 h; (B) substrate concentration for 0.6 μg of protein from human nSMase2-containing cell lysate for 1 h; and (C) time in the ...
Justin C. McArthur (66539) +11 more
core +1 more source
Journal of Extracellular Biology, Volume 5, Issue 9, September 2026.Created with BioRender.com. ABSTRACT Extracellular vesicles (EVs) are widely studied as mediators of intercellular communication, yet their quantitative characterization remains inconsistent. In this study, we investigated how vesicle release rate, cellular proliferation, and tumour origin jointly shape EV‐associated protein output. Using a panel of 19
Tímea Böröczky +9 more
wiley +1 more source
Nano Select, Volume 7, Issue 9, September 2026.Extracellular vesicles at the nexus of skin cancer progression and precision therapy. EVs orchestrate tumor microenvironment remodeling through cargo‐mediated intercellular communication, immune modulation, angiogenesis, and extracellular matrix reorganization, while also emerging as next‐generation platforms for targeted drug delivery, biomarker ...
Megha Kotian +7 more
wiley +1 more source
, 2019 Recently, extracellular vesicles (EVs), such as exosomes, have been proposed to play an influential role in the cell-to-cell spread of neurodegenerative diseases, including the intercellular transmission of α-synuclein (α-syn). However, the regulation of
Livia Civitelli (153987) +6 more
core +1 more source
Temporal changes in plasma membrane lipid content induce endocytosis to regulate developmental epithelial-to-mesenchymal transition [PDF]
, 2022 Epithelial-to-mesenchymal transition (EMT) is a dramatic change in cellular physiology during development and metastasis, which requires coordination between cell signaling, adhesion, and membrane protrusions.
Piacentino, Michael L +7 more
core +1 more source