Results 41 to 50 of about 1,883 (170)
Expression and characterization of recombinant human nSMase2.
, 2015 (A) Western blot showing expression of recombinant human nSMase2-Flag tagged construct in stably transfected HEK293 cells. Positive control corresponds to GST-tagged human nSMase2 construct commercially available.
Justin C. McArthur (66539) +11 more
core +1 more source
, 2023 Abstract Background Cognitive decline in Alzheimer’s disease (AD) is associated with prion-like tau propagation between neurons along synaptically connected networks, in part via extracellular vesicles (EV). EV biogenesis is triggered by ceramide enrichment at the plasma membrane from neutral sphingomyelinase2(nSMase2)-mediated cleavage of ...
Carolyn Tallon +16 more
openaire +2 more sources
Fluorescence and radioactivity assays for human nSMase2.
, 2015 (A) nSMase2-catalyzed reaction in the direct confirmatory assay with 14C-labeled SM (labeled atoms shown in magenta), which is hydrolyzed to generate labeled product. (B) Fluorescence assay for human nSMase2 consisting of three coupled reactions (in blue)
Justin C. McArthur (66539) +11 more
core +1 more source
nSMase2 (Type 2-Neutral Sphingomyelinase) Deficiency or Inhibition by GW4869 Reduces Inflammation and Atherosclerosis in Apoe−/−Mice [PDF]
Arteriosclerosis, Thrombosis, and Vascular Biology, 2018 Objective—Atherosclerosis is a chronic multifactorial and inflammatory disease of large and medium arteries and the leading cause of cardiovascular diseases worldwide. The aim of this study was to investigate whether and how the nSMase2 (type 2-neutral sphingomyelinase), a key enzyme of sphingolipid metabolism, may contribute to the development of ...
Lallemand, Tom +10 more
openaire +3 more sources
British Journal of Pharmacology, EarlyView.Extracellular vesicles (EVs) are a diverse population of membrane nanoparticles secreted by nearly all cell types, playing a key role in intercellular communication by transferring bioactive macromolecular cargo. In cancer, EVs shape both the local tumour microenvironment and distant premetastatic niches.
Evangelia Pantazaka +3 more
wiley +1 more source
Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes
Journal of Lipid Research, 2018 We reported that amyloid β peptide (Aβ42) activated neutral SMase 2 (nSMase2), thereby increasing the concentration of the sphingolipid ceramide in astrocytes.
Ji-Na Kong +13 more
doaj +1 more source
Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin [PDF]
, 2011 名古屋大学NAGOYA University博士(医療技術学)Mg^2+-dependent neutral SMases (NSMases) have emerged as prime candidates for stress-induced ceramide production. Among isoforms identified, previous reports have suggested the importance of NSMase2. However, its activation
伊藤, 裕美
core
Inhibition of human nSMase2 by cambinol and closely related analogs.
, 2015 (A) Cambinol (compound 1) structure. (B) Dose response curve for inhibition of human nSMase2 by increasing concentrations of cambinol ([14C]-SM assay). (C) Non-linear least squares fit of the Michaelis-Menten equation for the activity of human nSMase2 vs.
Justin C. McArthur (66539) +11 more
core +1 more source
Understanding extracellular vesicles in antiaging
Periodontology 2000, EarlyView.Abstract Background Aging is a multifactorial process marked by gradual cellular dysregulation, regulated by hallmarks, including genomic instability, mitochondrial dysregulation, and chronic inflammation. Extracellular vesicles (EVs), especially exosomes, have emerged as pivotal mediators of cell‐to‐cell communication with potential roles in both ...
Paras Ahmad +3 more
wiley +1 more source
Exosomes in Cancer: The Biology, Function, Diagnosis, and Therapy
MedComm, Volume 7, Issue 11, November 2026.Tumor‐derived exosomes and nonmembranous extracellular nanoparticles (exomeres/supermeres) constitute a heterogeneous communication network that drives cancer progression. By shuttling functional biomolecules across tissue‐specific barriers, these nanoparticles orchestrate local microenvironmental remodeling, immune suppression, premetastatic niche ...
Jun Wu +8 more
wiley +1 more source