Results 81 to 90 of about 3,842 (144)
The Coronavirus helicase in replication
Virus ResearchThe coronavirus nonstructural protein (nsp) 13 encodes an RNA helicase (nsp13-HEL) with multiple enzymatic functions, including unwinding and nucleoside phosphatase (NTPase) activities.
Samantha L. Grimes, Mark R. Denison
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Architecture of a SARS-CoV-2 mini replication and transcription complex
Nature Communications, 2020 SARS-CoV-2 virus replication and transcription is mediated by the replication and transcription complex (RTC) that is composed of 16 non-structural proteins (nsp). Here, the authors present the cryo-EM structure of a SARS-CoV-2 mini RTC consisting of the
Liming Yan +12 more
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Novel Tetrazolium-Based Colorimetric Assay for Helicase nsp13 in SARS-CoV-2
BioChemSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a human pathogenic virus that encodes for a helicase (SC2Hel) that is essential for viral replication. SC2Hel has the ability to unravel dsRNA or dsDNA in an NTP-dependent manner from the 5′
Triet M. Pham +4 more
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Heterogeneous Evolution Among SARS‐CoV‐2 Genes and Variants of Concern
Journal of Medical Virology, Volume 97, Issue 9, September 2025.ABSTRACT Challenges persist regarding the influence of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) on public health, with growing interest in future viral molecular variants. In this context, accurate predictions demand a thorough understanding of the virus's molecular evolution, especially proteins targeted by therapies, where ...
Luis Daniel González‐Vázquez +3 more
wiley +1 more source
Virus ResearchCoronaviruses contain one of the largest genomes among the RNA viruses, coding for 14–16 non-structural proteins (nsp) that are involved in proteolytic processing, genome replication and transcription, and four structural proteins that build the core of ...
Maria Kuzikov +13 more
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Angewandte Chemie, Volume 137, Issue 33, August 11, 2025.We show that antisense oligonucleotides can be used to restructure secondary structural elements around the circular DNA template binding sites of the genomic RNA for subsequent rolling circle amplification. This strategy was employed to identify otherwise COVID “false negative” saliva samples by accurately detecting the presence of SARS‐CoV‐2 genomic ...
Amal Mathai +4 more
wiley +1 more source
A Repurposed Drug Interferes with Nucleic Acid to Inhibit the Dual Activities of Coronavirus Nsp13
ACS Chemical BiologyThe recent pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlighted a critical need to discover more effective antivirals. While therapeutics for SARS-CoV-2 exist, its nonstructural protein 13 (Nsp13) remains a clinically untapped target.
Nathan Soper +9 more
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The PKA-CREB1 axis regulates coronavirus proliferation by viral helicase nsp13 association
Journal of VirologyABSTRACT The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed a worldwide threat in the past 3 years. Although it has been widely and intensively investigated, the mechanism underlying the coronavirus-host interaction requires further elucidation, which may contribute
Tao Zheng +14 more
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High throughput screening for SARS-CoV-2 helicase inhibitors
SLAS DiscoverySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for nearly 7 million deaths worldwide since its outbreak in late 2019. Even with the rapid development and production of vaccines and intensive research, there is still a huge ...
Yuka Otsuka +11 more
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